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Product Info Summary
| SKU: | EK2154 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CD300E ELISA Kit PicoKine®
View all CD300e/LMIR6 ELISA kits
SKU/Catalog Number
EK2154
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD300E ELISA Kit PicoKine® (96 Tests). Quantitate Human CD300E in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD300E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2154)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: HEK293; Immunogen sequence: L18-L169
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2154 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2154 is reactive to CD300E in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2154 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD300e/LMIR6
This gene encodes a member of the CD300 glycoprotein family of cell surface proteins expressed on myeloid cells. The protein interacts with the TYRO protein tyrosine kinase-binding protein and is thought to act as an activating receptor.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2154-CAP | Anti-Human CD300E Pre-coated 96-well strip microplate | 1 |
| EK2154-ST | Human CD300E Standard | 2 vials, 10 ng/tube |
| EK2154-DA | Human CD300E Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.014 | 0.056 | 0.108 | 0.277 | 0.461 | 0.870 | 1.652 | 2.319 |
Data Example Images
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Human CD300E PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD300E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 84 | 336 | 684 | 106 | 396 | 723 |
| Standard deviation | 4.7 | 20.16 | 34.88 | 5.19 | 13.07 | 37.6 |
| CV(%) | 5.6% | 6% | 5.1% | 4.9% | 3.3% | 5.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD300e/LMIR6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 84 | 85 | 88 | 92 | 87 | 2.88 | 3.3% |
| Sample 2 | 336 | 342 | 365 | 351 | 349 | 7.32 | 2.1% |
| Sample 3 | 684 | 721 | 696 | 682 | 696 | 24.35 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For CD300E (Source: Uniprot.Org, NCBI)
Gene Name
CD300E
Full Name
CMRF35-like molecule 2
Weight
21.822kDa
Superfamily
CD300 family
Alternative Names
CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD300E, check out the CD300E Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD300E: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD300E ELISA Kit PicoKine® (EK2154)
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