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Product Info Summary
| SKU: | EK2147 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
Product info
Product Name
Human CD109 ELISA Kit PicoKine®
SKU/Catalog Number
EK2147
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD109 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD109 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD109 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2147)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: V22-S1268
Sensitivity
<20 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2147 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2147 is reactive to CD109 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
EK2147 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD109
This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2147-CAP | Anti-Human CD109 Pre-coated 96-well strip microplate | 1 |
| EK2147-ST | Human CD109 Standard | 2 vials, 20 ng/tube |
| EK2147-DA | Human CD109 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.030 | 0.074 | 0.152 | 0.292 | 0.451 | 0.968 | 1.441 | 2.152 |
Data Example Images
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Human CD109 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD109 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 468 | 1652 | 4527 | 521 | 1699 | 4651 |
| Standard deviation | 15.44 | 57.82 | 276.15 | 23.45 | 54.37 | 279.06 |
| CV(%) | 3.3% | 3.5% | 6.1% | 4.5% | 3.2% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD109 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 468 | 470 | 489 | 505 | 483 | 21.74 | 4.5% |
| Sample 2 | 1652 | 1744 | 1825 | 1697 | 1730 | 72.64 | 4.2% |
| Sample 3 | 4527 | 4652 | 4741 | 4426 | 4587 | 288.95 | 6.3% |
Protein Target Info & Infographic
Gene/Protein Information For CD109 (Source: Uniprot.Org, NCBI)
Gene Name
CD109
Full Name
CD109 antigen
Weight
Superfamily
protease inhibitor I39 (alpha-2-macroglobulin) family
Alternative Names
150 kDa TGF-beta-1-binding protein; activated T-cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109 antigen (Gov platelet alloantigens); CD109 antigen; CD109 molecule; CD109; CPAMD7; CPAMD7r150; DKFZp762L1111; FLJ38569; FLJ41966; Gov platelet alloantigens; p180; Platelet-specific Gov antigen; RP11-525G3.1 CD109 CPAMD7, p180, r150 CD109 molecule CD109 antigen|150 kDa TGF-beta-1-binding protein|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7|Gov platelet alloantigens|activated T-cell marker CD109|platelet-specific Gov antigen
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD109, check out the CD109 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD109: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD109 ELISA Kit PicoKine® (EK2147)
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