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Product Info Summary
| SKU: | FEK0450 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CCL4/MIP-1 beta PicoKine® Quick ELISA Kit
See all CCL4/MIP-1 beta products
SKU/Catalog Number
FEK0450
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CCL4/MIP-1 beta PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL4/MIP-1 beta in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CCL4/MIP-1 beta PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0450)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A24-N92
Sensitivity
<4 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0450 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0450 is reactive to CCL4 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
FEK0450 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL4/MIP-1 beta
Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0450-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0450-ST | Human CCL4/MIP-1 beta Standard | 2 | 10 ng/tube |
| FEK0450-A | Human CCL4 Antibody A | 1 | 3ml |
| FEK0450-B | Human CCL4 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.020 | 0.107 | 0.182 | 0.309 | 0.576 | 1.050 | 1.825 | 2.001 |
Data Example Images
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Human CCL4 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CCL4/MIP-1 beta PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 45 | 333 | 503 | 39 | 341 | 577 |
| Standard deviation | 1.93 | 22.97 | 39.73 | 2.41 | 25.57 | 50.77 |
| CV(%) | 4.3% | 6.9% | 7.9% | 6.2% | 7.5% | 8.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL4/MIP-1 beta in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 45 | 47 | 40 | 41 | 43 | 1.55 | 3.8% |
| Sample 2 | 333 | 237 | 173 | 219 | 240 | 18.48 | 7.7% |
| Sample 3 | 503 | 533 | 481 | 465 | 495 | 26.23 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For CCL4 (Source: Uniprot.Org, NCBI)
Gene Name
CCL4
Full Name
C-C motif chemokine 4
Weight
10.212kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2 CCL4 ACT2, AT744.1, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4 C-C motif chemokine ligand 4 C-C motif chemokine 4|G-26 T-lymphocyte-secreted protein|MIP-1-beta(1-69)|PAT 744|SIS-gamma|T-cell activation protein 2|chemokine (C-C motif) ligand 4|lymphocyte activation gene 1 protein|macrophage inflammatory protein 1-beta|secreted protein G-26|small inducible cytokine A4 (homologous to mouse Mip-1b)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL4, check out the CCL4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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