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Product Info Summary
| SKU: | PB9031 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TARC/CCL17 Antibody Picoband®
View all CCL17/TARC Antibodies
SKU/Catalog Number
PB9031
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TARC/CCL17 Antibody Picoband® catalog # PB9031. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TARC/CCL17 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9031)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse TARC recombinant protein (Position: A34-P103). Mouse TARC shares 71% amino acid (aa) sequence identity with human TARC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9031 is reactive to Ccl17 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
10467 MW
Background of CCL17/TARC
Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1. The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9031 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, -
ELISA, 0.1-0.5μg/ml, -
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TARC using anti-TARC antibody (PB9031).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Mouse TARC Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARC antigen affinity purified polyclonal antibody (Catalog # PB9031) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TARC at approximately 34KD. The expected band size for TARC is at 34KD.
Protein Target Info & Infographic
Gene/Protein Information For Ccl17 (Source: Uniprot.org, NCBI)
Gene Name
Ccl17
Full Name
C-C motif chemokine 17
Weight
10467 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine ;Ccl17 ;ABCD-2 , TARC ;mCG_12286 ; CCL17 A-152E5.3, ABCD-2, SCYA17, TARC C-C motif chemokine ligand 17 C-C motif chemokine 17|CC chemokine TARC|T cell-directed CC chemokine|chemokine (C-C motif) ligand 17|small inducible cytokine subfamily A (Cys-Cys), member 17|small-inducible cytokine A17|thymus and activation-regulated chemokine
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccl17, check out the Ccl17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccl17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TARC/CCL17 Antibody Picoband® (PB9031)
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No publications found for PB9031
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Customer Q&As
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1 Customer Q&As for Anti-TARC/CCL17 Antibody Picoband®
Question
We are currently using anti-TARC/CCL17 antibody PB9031 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
The anti-TARC/CCL17 antibody (PB9031) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-26
