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Product Info Summary
| SKU: | FEK1929 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human Cathepsin V PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1929
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cathepsin V PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human CTSV in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human Cathepsin V PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1929)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: V18-V334
Sensitivity
<15 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1929 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1929 is reactive to CTSV in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1929 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cathepsin V
Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1929-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1929-ST | Human Cathepsin V Standard | 2 | 10 ng/tube |
| FEK1929-A | Human CTSV Antibody A | 1 | 3ml |
| FEK1929-B | Human CTSV Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.096 | 0.132 | 0.216 | 0.379 | 0.609 | 1.011 | 1.828 | 2.542 |
Data Example Images
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Human ANGPTL7 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cathepsin V PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 100 | 714 | 2346 | 136 | 823 | 2464 |
| Standard deviation | 5.30 | 44.27 | 105.57 | 6.39 | 52.67 | 133.06 |
| CV (%) | 5.3% | 6.2% | 4.5% | 4.7% | 6.4% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cathepsin V in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 100 | 121 | 111 | 116 | 112 | 7.77 | 6.9% |
| Sample 2 | 741 | 756 | 857 | 851 | 801 | 53.05 | 6.6% |
| Sample 3 | 2346 | 2384 | 2464 | 2369 | 2390 | 44.40 | 1.8% |
Protein Target Info & Infographic
Gene/Protein Information For CTSV (Source: Uniprot.Org, NCBI)
Gene Name
CTSV
Full Name
Cathepsin L2
Weight
37.329kDa
Superfamily
peptidase C1 family
Alternative Names
Cathepsin L2; Cathepsin U; Cathepsin V; CATL2; CTSL2; CTSUcathepsin L2, preproprotein; CTSVMGC125957; EC 3.4.22; EC 3.4.22.43 CTSV CATL2, CTSL2, CTSU cathepsin V cathepsin L2|cathepsin L2, preproprotein|cathepsin U
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CTSV, check out the CTSV Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTSV: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Cathepsin V PicoKine® Quick ELISA Kit (FEK1929)
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