Human/Canine/Porcine Insulin ELISA Kit PicoKine®

INS ELISA kit for Canine, Human, Porcine

Human/Canine/Porcine Insulin ELISA Kit PicoKine® (96 Tests). Quantitate Human/canine/porcine INS in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 2 pmol/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK2287
Size: 96 wells/kit, with removable strips.
Reactive Species: Canine, Human, Porcine
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human/Canine/Porcine Insulin ELISA Kit PicoKine®

View all INS ELISA kits

SKU/Catalog Number

EK2287

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human/Canine/Porcine Insulin ELISA Kit PicoKine® (96 Tests). Quantitate Human/canine/porcine INS in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 2 pmol/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human/Canine/Porcine Insulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2287)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: F25-N110

Sensitivity

<2 pmol/L

Assay Range

15.6 pmol/L - 1,000 pmol/L

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK2287 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2287 is reactive to INS in Canine, Human, Porcine samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2287 is guaranteed for ELISA in Canine, Human, Porcine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of INS

Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the insulin (INS) gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

Kit Components

Catalog Number Description Quantity
EK2287-CAP Anti-Canine, Human, Porcine INS Pre-coated 96-well strip microplate 1
EK2287-ST Canine, Human, Porcine INS Standard 2 vials, 1 pmol/tube
EK2287-DA Canine, Human, Porcine INS Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration(pmol/L)015.631.262.51252505001,000
O.D.0.0460.0840.1970.2810.4610.8881.2512.012

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human/Canine/Porcine Insulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pmol/L)289347425118274
Standard deviation1.532.7210.571.145.1721.81
CV(%)5.5%2.9%2.2%4.5%4.4%8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of INS in ELISA kits from four different production batches/lots.

LotsLot 1 (pmol/L)Lot 2 (pmol/L)Lot 3 (pmol/L)Lot 4 (pmol/L)Mean (pmol/L)Standard DeviationCV (%)
Sample 128182419221.697.6%
Sample 2939311285966.967.3%
Sample 347441129944440721.085.2%
*number of samples for each test n=16.

Gene/Protein Information For INS (Source: Uniprot.Org, NCBI)

Gene Name

INS

Full Name

Insulin

Weight

11.981kDa

Superfamily

insulin family

Alternative Names

IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin INS IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4 insulin insulin|preproinsulin|proinsulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on INS, check out the INS Infographic

INS infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for INS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EK2287

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human/Canine/Porcine Insulin ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Human/Canine/Porcine Insulin ELISA Kit PicoKine®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Human/Canine/Porcine Insulin ELISA Kit PicoKine®

$499
Backordered.

Lead time for this item is typically 2-3 weeks

Get A Quote
In stock
Order Product
EK2287
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.