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Product Info Summary
| SKU: | EK2287 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Canine, Human, Porcine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human/Canine/Porcine Insulin ELISA Kit PicoKine®
SKU/Catalog Number
EK2287
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human/Canine/Porcine Insulin ELISA Kit PicoKine® (96 Tests). Quantitate Human/canine/porcine INS in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 2 pmol/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human/Canine/Porcine Insulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2287)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: F25-N110
Sensitivity
<2 pmol/L
Assay Range
15.6 pmol/L - 1,000 pmol/L
Standard Dilution Instructions
See datasheet of EK2287 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2287 is reactive to INS in Canine, Human, Porcine samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2287 is guaranteed for ELISA in Canine, Human, Porcine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of INS
Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the insulin (INS) gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2287-CAP | Anti-Canine, Human, Porcine INS Pre-coated 96-well strip microplate | 1 |
| EK2287-ST | Canine, Human, Porcine INS Standard | 2 vials, 1 pmol/tube |
| EK2287-DA | Canine, Human, Porcine INS Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pmol/L) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.046 | 0.084 | 0.197 | 0.281 | 0.461 | 0.888 | 1.251 | 2.012 |
Data Example Images
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Human/Canine/Porcine Insulin ELISA Kit PicoKine standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human/Canine/Porcine Insulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pmol/L) | 28 | 93 | 474 | 25 | 118 | 274 |
| Standard deviation | 1.53 | 2.72 | 10.57 | 1.14 | 5.17 | 21.81 |
| CV(%) | 5.5% | 2.9% | 2.2% | 4.5% | 4.4% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of INS in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pmol/L) | Lot 2 (pmol/L) | Lot 3 (pmol/L) | Lot 4 (pmol/L) | Mean (pmol/L) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 28 | 18 | 24 | 19 | 22 | 1.69 | 7.6% |
| Sample 2 | 93 | 93 | 112 | 85 | 96 | 6.96 | 7.3% |
| Sample 3 | 474 | 411 | 299 | 444 | 407 | 21.08 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For INS (Source: Uniprot.Org, NCBI)
Gene Name
INS
Full Name
Insulin
Weight
11.981kDa
Superfamily
insulin family
Alternative Names
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin INS IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4 insulin insulin|preproinsulin|proinsulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on INS, check out the INS Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for INS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human/Canine/Porcine Insulin ELISA Kit PicoKine® (EK2287)
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