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Product Info Summary
| SKU: | EK2192 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human Calsyntenin 1 ELISA Kit PicoKine®
View all Calsyntenin-1 ELISA kits
SKU/Catalog Number
EK2192
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Calsyntenin 1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CLSTN1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Calsyntenin 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2192)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: R30-T851
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2192 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2192 is reactive to CLSTN1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2192 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Calsyntenin-1
Calsyntenin-1 is a protein that in humans is encoded by the CLSTN1 gene. This gene is a member of the calsyntenin family, a subset of the cadherin superfamily. The encoded transmembrane protein, also known as alcadein-alpha, is thought to bind to kinesin-1 motors to mediate the axonal anterograde transport of certain types of vesicle. Amyloid precursor protein (APP) is trafficked via these vesicles and so this protein is being investigated to see how it might contribute to the mechanisms underlying Alzheimer's disease. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2192-CAP | Anti-Human CLSTN1 Pre-coated 96-well strip microplate | 1 |
| EK2192-ST | Human CLSTN1 Standard | 2 vials, 10 ng/tube |
| EK2192-DA | Human CLSTN1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.016 | 0.048 | 0.135 | 0.251 | 0.444 | 0.896 | 1.274 | 2.056 |
Data Example Images
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Human Calsyntenin 1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Calsyntenin 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 23 | 163 | 452 | 21 | 174 | 423 |
| Standard deviation | 1.27 | 11.25 | 32.09 | 1.09 | 9.22 | 25.8 |
| CV(%) | 5.5% | 6.9% | 7.1% | 5.2% | 5.3% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Calsyntenin-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 23 | 22 | 19 | 20 | 21 | 0.76 | 3.6% |
| Sample 2 | 163 | 165 | 158 | 156 | 161 | 6.74 | 4.2% |
| Sample 3 | 452 | 463 | 456 | 470 | 460 | 20.71 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For CLSTN1 (Source: Uniprot.Org, NCBI)
Gene Name
CLSTN1
Full Name
Calsyntenin-1
Weight
109.793kDa
Alternative Names
alcadein alpha 1; Alcadein-alpha; ALC-ALPHA; alcalpha1; alcalpha2; Alzheimer-related cadherin-like protein; cadherin-related family member 12; calsyntenin 1; Calsyntenin1; Calsyntenin-1; CDHR12; CLSTN1; CS1; CSTN1; FLJ32258; KIAA0911alcadein-alpha; Non-classical cadherin XB31alpha; non-classical cadherin XB31alpha1; PIK3CD; XB31alpha CLSTN1 ALC-ALPHA, CDHR12, CST-1, CSTN1, PIK3CD, XB31alpha, alcalpha1, alcalpha2 calsyntenin 1 calsyntenin-1|alcadein-alpha|alzheimer-related cadherin-like protein|cadherin-related family member 12|non-classical cadherin XB31alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CLSTN1, check out the CLSTN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CLSTN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Calsyntenin 1 ELISA Kit PicoKine® (EK2192)
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