Human CA15-3 ELISA Kit

Product Name

Human CA15-3 ELISA Kit

See all MUC1 products

SKU/Catalog Number

EK7030

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA15-3. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CA15-3 ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7030)

Clonality of Antibodies

See Datasheet for details

Sensitivity

3.8U/ml

Assay Range

10-300U/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7030 is reactive to MUC1 in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7030 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Anti-Human CA15-3 Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Anti- CA15-3 monoclonal antibody, Polystyrene micro-well plate
Human CA15-3 Standards (S1~S4)	4	0.5ml	CA15-3 (from natural protein) (10，50，150, 300) U/ml, 0.02M PBS, 20% new-born calf serum
HRP Conjugated anti-Human CA15-3 antibody	1	11ml	HRP Conjugated anti-Human CA15-3 antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, from mouse monoclonal antibody
Sample diluent	1	11ml	0.02M PBS, 20% new-born calf serum
Controls	1	0.5ml	CA15-3 (from natural protein) U/ml, 0.02M PBS, 20% new-born calf serum
20X Wash Buffer Concentrate	1	15ml	Neutral buffer, contains 0.5% Tween-20
Color Developing Reagent A	1	7ml	Contains 11mmol/L H2O2
Color Developing Reagent B	1	7ml	Contains 2mmol/L TMB
Stop Solution	1	7ml	2mol/L dilute sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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