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Product Info Summary
| SKU: | EK2288 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human C-Peptide ELISA Kit PicoKine®
SKU/Catalog Number
EK2288
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human C-Peptide ELISA Kit PicoKine® (96 Tests). Quantitate Human C-Peptide in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human C-Peptide ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2288)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: human peptide
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2288 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2288 is reactive to in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2288 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of
C-Peptide, or connecting peptide, is a short peptide chain that is produced during the synthesis of insulin. When proinsulin is cleaved to form insulin, C-Peptide is released as a byproduct. It serves as a useful biomarker for assessing insulin production in the body because its levels reflect the amount of insulin secreted by the pancreas. Unlike insulin, C-Peptide has a longer half-life in the bloodstream, making it easier to measure. Elevated levels of C-Peptide can indicate insulinoma or insulin resistance, while low levels may suggest type 1 diabetes or pancreatic dysfunction. Understanding C-Peptide levels can help in diagnosing and managing various metabolic disorders.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2288-CAP | Anti-Human Pre-coated 96-well strip microplate | 1 |
| EK2288-ST | Human Standard | 2 vials, 2 ng/tube |
| EK2288-DA | Human Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.042 | 0.089 | 0.125 | 0.248 | 0.484 | 0.681 | 1.187 | 2.022 |
Data Example Images
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Human C-Peptide ELISA Kit PicoKine standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human C-Peptide ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 69 | 318 | 1720 | 92 | 351 | 1533 |
| Standard deviation | 1.6 | 17.84 | 50.05 | 4.66 | 13.3 | 66.84 |
| CV(%) | 2.3% | 5.6% | 2.9% | 5.1% | 3.8% | 4.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 69 | 72 | 106 | 71 | 80 | 5.23 | 6.6% |
| Sample 2 | 318 | 500 | 455 | 447 | 430 | 15.01 | 3.5% |
| Sample 3 | 1720 | 1706 | 1260 | 1331 | 1504 | 117.63 | 7.8% |
Specific Publications For Human C-Peptide ELISA Kit PicoKine® (EK2288)
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