Product Info Summary
SKU: | EK2035 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human ANGPTL7 ELISA Kit PicoKine®
View all Angiopoietin-like Protein 7/ANGPTL7 ELISA kits
SKU/Catalog Number
EK2035
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ANGPTL7 ELISA Kit PicoKine® (96 Tests). Quantitate Human ANGPTL7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ANGPTL7 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2035)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q27-P346
Sensitivity
<50 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2035 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2035 is reactive to ANGPTL7 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2035 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angiopoietin-like Protein 7/ANGPTL7
Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 1p36.22. Angiopoietin-like Protein 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 45‑50 kDa glycoprotein member of the angiopoietin‑like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. ANGPTL7 expression is up‑regulated under conditions of increased ocular pressure.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2035-CAP | Anti-Human ANGPTL7 Pre-coated 96-well strip microplate | 1 |
EK2035-ST | Human ANGPTL7 Standard | 2 vials, 10 ng/tube |
EK2035-DA | Human ANGPTL7 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.044 | 0.109 | 0.162 | 0.268 | 0.480 | 0.828 | 1.459 | 2.048 |
Data Example Images
Click image to see more details
Human ANGPTL7 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Angiopoietin-like Protein 7/ANGPTL7, Dilution ratio of 1:1, concentration in serum and plasma is around 300 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ANGPTL7 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 140 | 644 | 1907 | 145 | 639 | 2253 |
Standard deviation | 6.86 | 27.69 | 97.26 | 9.57 | 35.15 | 173.48 |
CV (%) | 4.9% | 4.3% | 5.1% | 6.6% | 5.5% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 7/ANGPTL7 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 140 | 129 | 118 | 129 | 129 | 7.77 | 6.0% |
Sample 2 | 644 | 660 | 702 | 583 | 647 | 42.71 | 6.6% |
Sample 3 | 1907 | 2152 | 2078 | 2215 | 2088 | 115.20 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For ANGPTL7 (Source: Uniprot.Org, NCBI)
Gene Name
ANGPTL7
Full Name
Angiopoietin-related protein 7
Weight
40.018kDa
Alternative Names
Angiopoietin-related protein 7; Angiopoietin-like factor; Angiopoietin-like protein 7; Cornea-derived transcript 6 protein; ANGPTL7; CDT6; UNQ313; PRO356 ANGPTL7 AngX, CDT6, dJ647M16.1 angiopoietin like 7 angiopoietin-related protein 7|angiopoietin-like factor (CDT6)|angiopoietin-like protein 7|cornea-derived transcript 6 protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANGPTL7, check out the ANGPTL7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANGPTL7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ANGPTL7 ELISA Kit PicoKine® (EK2035)
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Customer Q&As
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7 Customer Q&As for Human ANGPTL7 ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I measured ANGPTL7 level in serum.
M. Anderson
Verified customer
Asked: 2020-09-09
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-09-09
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring ANGPTL7 using the EK2035 Human ANGPTL7 Picokine® ELISA Kit?
H. Jha
Verified customer
Asked: 2020-05-03
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-05-03
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-12-22
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
G. Scott
Verified customer
Asked: 2019-08-13
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-13
Question
Q: is it okay to use citrate plasma as samples in Human ANGPTL7 Picokine® ELISA Kit (Catalog # EK2035)?
D. Patel
Verified customer
Asked: 2019-01-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of ANGPTL7 causing degradation of its protein structure. ANGPTL7 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the ANGPTL7 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-01-26
Question
Q: What is the optimal O.D. value for ANGPTL7 ELISA kit? I performed your ANGPTL7 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ANGPTL7 even though the O.D. values are not very high?
S. Patel
Verified customer
Asked: 2018-05-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ANGPTL7 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of ANGPTL7 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-05-10
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
H. Surve
Verified customer
Asked: 2018-03-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-03-02