Product Info Summary
SKU: | EK2137 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human ACHE ELISA Kit PicoKine®
View all Acetylcholinesterase/ACHE ELISA kits
SKU/Catalog Number
EK2137
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ACHE ELISA Kit PicoKine® (96 Tests). Quantitate Human ACHE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ACHE ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2137)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: E32-L614
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2137 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2137 is reactive to ACHE in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2137 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Acetylcholinesterase/ACHE
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2137-CAP | Anti-Human ACHE Pre-coated 96-well strip microplate | 1 |
EK2137-ST | Human ACHE Standard | 2 vials, 10 ng/tube |
EK2137-DA | Human ACHE Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
O.D. | 0.060 | 0.152 | 0.233 | 0.358 | 0.594 | 0.883 | 1.402 | 2.035 |
Data Example Images
Click image to see more details
Human ACHE PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Acetylcholinesterase/ACHE, Dilution ratio of 1:10, concentration in serum and plasma is around 500 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ACHE ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 46 | 163 | 278 | 52 | 178 | 305 |
Standard deviation | 2.07 | 7.66 | 9.17 | 2.7 | 10.32 | 15.86 |
CV(%) | 4.5% | 4.7% | 3.3% | 5.2% | 5.8% | 5.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Acetylcholinesterase/ACHE in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 46 | 51 | 39 | 59 | 49 | 1.61 | 3.3% |
Sample 2 | 163 | 188 | 174 | 150 | 169 | 7.76 | 4.6% |
Sample 3 | 278 | 312 | 333 | 329 | 313 | 18.47 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For ACHE (Source: Uniprot.Org, NCBI)
Gene Name
ACHE
Full Name
Acetylcholinesterase
Weight
67.796kDa
Superfamily
type-B carboxylesterase/lipase family
Alternative Names
ACHE; Acetylcholinesterase; AChE; EC 3.1.1.7 ACHE ACEE, ARACHE, N-ACHE, YT acetylcholinesterase (Cartwright blood group) acetylcholinesterase|Yt blood group|acetylcholinesterase (Yt blood group)|apoptosis-related acetylcholinesterase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ACHE, check out the ACHE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ACHE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ACHE ELISA Kit PicoKine® (EK2137)
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Customer Q&As
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2 Customer Q&As for Human ACHE ELISA Kit PicoKine®
Question
Is it possible to measure Butyrylcholinesterase (BChE) with this kit?
Verified customer
Asked: 2021-12-09
Answer
EK2137 Human ACHE ELISA Kit can not be used to measure Butyrylcholinesterase (BChE).
Boster Scientific Support
Answered: 2021-12-10
Question
If Q1 is no, do you have a kit that can measure Butyrylcholinesterase (BChE)?
Verified customer
Asked: 2021-12-09
Answer
Please use EK1507 Human BCHE ELISA Kit to measure Butyrylcholinesterase (BChE).
Boster Scientific Support
Answered: 2021-12-10