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Product Info Summary
| SKU: | BA1088 |
|---|---|
| Size: | 0.5ml |
| Application: | ELISA, IHC, WB |
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Product info
| Product Name | HRP Conjugated Streptavidin |
| SKU/Catalog Number | BA1088 |
| Pack Size | 0.5ml |
| Form | Concentrated, Liquid |
| Concentration | 1mg/ml |
| Product Description | Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-peroxidase can form a complex which composed by about one hundred peroxidase and fifty streptavidin. And lots of enzymes can ensure the streptavidin-peroxidase with high sensitivity. |
| Contents | 0.5 mg of HRP conjugated streptavidin 0.01 M PBS (pH7.4) 50% glycerol |
| Labeling Method | Streptavidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK. (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).) |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
| Applications | WB, IHC, ELISA |
| Cite This Product | Peroxidase Conjugated Streptavidin (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1088) |
Product Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blotting: 0.25-0.5μg/ml (ECL detection) Western Blotting: 2.5-5μg/ml (DAB detection) Immunohistochemistry: 2-10μg/ml ELISA: 0.01-0.1μg/ml (TMB detection)
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For BA1088
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BA1088 has been cited in 103 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Propofol alleviates hepatic ischemia/reperfusion injury via the activation of the Sirt1 pathway
The protective role of Schwann cells in bladder smooth muscle cell fibrosis
Clinicopathological correlation analysis of (lymph) angiogenesis and corneal graft rejection
Evidence for the Presence of Long-Lived Plasma Cells in Nasal Polyps
Effects of long non-coding RNA myocardial infarction-associated transcript on retinal neovascularization in a newborn mouse model of oxygen-induced retinopathy
A mimetic peptide of α2,6-sialyllactose promotes neuritogenesis
Suppression of midkine gene promotes the antitumoral effect of cisplatin on human gastric cancer cell line AGS in vitro and in vivo via the modulation of Notch signaling pathway
Expression and prognostic value of E2F3 transcription factor in non‑small cell lung cancer
Exosomes derived from endoplasmic reticulum‑stressed liver cancer cells enhance the expression of cytokines in macrophages via the STAT3 signaling pathway
Protective effects of ginsenoside Rg1 on aging Sca‑1+ hematopoietic cells
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