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Product Info Summary
| SKU: | BA1058 |
|---|---|
| Size: | 0.5ml |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Overview
| Product Name | HRP Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) |
|---|---|
| Synonyms | HRP-conjugated rabbit anti-rat IgG |
| Description | HRP Conjugated Rabbit Anti-rat IgG (H+L) (gamma-chain specific) secondary antibody This HRP conjugated antibody is specific for rat IgG and shows no cross-reactivity with human/bovine/goat/rabbit IgG. |
| Reagent Type | Secondary antibody, reporter enzyme labeled |
| Label | HRP (Horseradish Peroxidase) |
| Host | Rabbit |
| Target Species | Rat |
| Antibody Class | IgG |
| Clonality | Polyclonal |
| Immunogen | Rat IgG (whole molecular) |
| Purification | The antibody was purified from antisera by immunoaffinity chromatography. |
| Specificity | This HRP conjugated antibody is specific for rat IgG and shows no cross-reactivity with human/bovine/goat/rabbit IgG. |
| Form Supplied | Concentrated, Liquid |
| Formulation | 0.5 mg of HRP conjugated specific antibody
0.01 M PBS (PH 7.4) 50% glycerol |
| Pack Size | 0.5ml |
| Concentration | 1mg/ml |
| Application | ELISA*, WB* *Our Boster Guarantee covers the use of this product in the above marked tested applications. |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
| Precautions | FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE |
Assay Information
| Sample Type | SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates |
|---|---|
| Assay Type | Immunoassay |
| Assay Purpose | Protein detection/quantification |
| Technique | Immunodetection of target antibody with HRP reporter enzyme |
| Equipment Needed | WB/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer |
| Compatibility with Reagents | Incompatible with sodium azide and metals incompatible with high phosphate concentrations |
Main Advantages
| Specific | High signal-to-noise ratio |
|---|---|
| Sensitive | Detects low-abundant targets due to an optimal number of HRP molecules per antibody |
| High Signal Amplification | Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC |
| Fast | Generates strong signals in a relatively short time span |
| Quantifieable | Allows quantification of detected signal |
| Easy to Use | Supplied in a workable liquid format |
| Flexible | HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates; |
| Convenient | HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes |
Background
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.
Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.
Product Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection)
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For BA1058
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BA1058 has been cited in 110 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of rapamycin (RAPA) on the growth of lung cancer and its mechanism in mice with A549
Atorvastatin ameliorates myocardial ischemia/reperfusion injury through attenuation of endoplasmic reticulum stress-induced apoptosis
Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning
Slug mediates nasopharyngeal carcinoma radioresistance via downregulation of PUMA in a p53-dependent and -independent manner
Neuregulin 1 enhances cell adhesion molecule L1 like expression levels and promotes malignancy in human glioma
Advanced glycation end products induce proliferation, invasion and epithelial‑mesenchymal transition of human SW480 colon cancer cells through the PI3K/AKT signaling pathway
miR‑200b inhibits CD133+ glioma cells by targeting the AKT pathway
miR‑125a inhibits the migration and invasion of liver cancer cells via suppression of the PI3K/AKT/mTOR signaling pathway
SH2B1 protects against OGD/R‑induced apoptosis in PC12 cells via activation of the JAK2/STAT3 signaling pathway
Protection against monocrotaline-induced pulmonary arterial hypertension and caveolin-1 downregulation by fluvastatin in rats
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