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Product Info Summary
| SKU: | EK1440 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | All Animals |
| Application: | ELISA |
| Sample Types: | cell lysates. |
Product info
Product Name
GFP/Green fluorescent protein ELISA Kit PicoKine®
SKU/Catalog Number
EK1440
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
GFP/Green fluorescent protein ELISA Kit PicoKine® (96 Tests). Quantitate GFP in cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
GFP/Green fluorescent protein ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1440)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S2-K238
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1440 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1440 is reactive to GFP in All Animals samples
Validated Sample Types
cell lysates.
Application Guarantee
EK1440 is guaranteed for ELISA in All Animals by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GFP
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1440-CAP | Anti-All Animals GFP Pre-coated 96-well strip microplate | 1 |
| EK1440-ST | All Animals GFP Standard | 2 vials, 10 ng/tube |
| EK1440-DA | All Animals GFP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.047 | 0.072 | 0.154 | 0.303 | 0.599 | 1.091 | 1.843 |
Data Example Images
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GFP PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of GFP/Green fluorescent protein ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 39 | 220 | 444 | 38 | 224 | 429 |
| Standard deviation | 1.98 | 17.16 | 28.86 | 2.16 | 20.16 | 30.88 |
| CV (%) | 7.2% | 7.8% | 6.5% | 5.7% | 9% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GFP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 39 | 34 | 38 | 35 | 36 | 2.06 | 5.7% |
| Sample 2 | 220 | 216 | 221 | 238 | 223 | 8.43 | 3.7% |
| Sample 3 | 444 | 430 | 423 | 422 | 429 | 8.78 | 2% |
Specific Publications For GFP/Green fluorescent protein ELISA Kit PicoKine® (EK1440)
Hello CJ!
EK1440 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Colonization of Pinus radiata D. Don Seedling Roots by Biocontrol Bacteria Erwinia billingiae and Bacillus simplex
Steroid-sensitive nephrotic syndrome candidate gene CLVS1 regulates podocyte oxidative stress and endocytosis
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for GFP/Green fluorescent protein ELISA Kit PicoKine®
Question
May I know the Limit of Detection for GFP/Green Fluorescent Protein ELISA Kit PicoKine® #EK1440
Verified customer
Asked: 2022-12-12
Answer
The Limit of Detection for GFP/Green Fluorescent Protein ELISA Kit PicoKine® #EK1440 is 15.6 pg/ml. The detection range is 15.6 pg/ml - 1,000 pg/ml.
Boster Scientific Support
Answered: 2022-12-12
Question
Are there any additional recommendations for using EK1440 with mouse brain samples?
Verified customer
Asked: 2022-05-19
Answer
Our lab hasn't worked on tissue samples with the GFP/Green Fluorescent Protein ELISA Kit PicoKine® (EK1440). Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend running a pilot test as described below to confirm the affect of endogeous biotin first; Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit. The background signal generated by endogenous biotin in the samples should be considered.
Boster Scientific Support
Answered: 2022-05-20
Question
Would EK1440 work for an EGFP protein?
Verified customer
Asked: 2022-03-31
Answer
Yes, GFP/Green Fluorescent Protein ELISA Kit PicoKine® (EK1440) works for an EGFP protein.
Boster Scientific Support
Answered: 2022-04-01
Question
Is EK1440 based on a polyclonal/monoclonal or monoclonal/monoclonal combination?
Verified customer
Asked: 2022-03-31
Answer
For GFP/Green Fluorescent Protein ELISA Kit PicoKine® (EK1440), the capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Boster Scientific Support
Answered: 2022-04-01
Question
Can EK1440 detect YFP/GFP in plants?
Verified customer
Asked: 2021-11-12
Answer
Our lab hasn't detected YFP/GFP in plants using the GFP/Green Fluorescent Protein ELISA Kit PicoKine® (EK1440). We are uncertain if the kit is able to detect YFP/GFP in plants. We suggest running a pilot test to see if it works. In theory, if the expression level of GFP in the samples falls in the assay range of the kit, the kit is able to detect the protein in the sample.
Boster Scientific Support
Answered: 2021-11-12
Question
Q: What is the optimal O.D. value for GFP ELISA kit? I used your GFP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain GFP even though the O.D. values are not very high?
L. Collins
Verified customer
Asked: 2020-08-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GFP ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of GFP that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-08-03
Question
Q: Can GFP ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
B. Wang
Verified customer
Asked: 2020-07-30
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-07-30
Question
A customer wanted to check to see if there is a separate protocol for E.coli cell lysates? Or would the protocol on the datasheet be fine?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Protein presents as supernatant 1. Centrifuge to collect E.coli. 2. Resuspend E.coli in PBS. 3. Sonicate to break up E.coli. 4. Centrifuge the lysates and collect the supernatant. Protein presents as inclusion body 1. Centrifuge to collect E.coli. 2. Resuspend E.coli in PBS. 3. Sonicate to break up E.coli. 4. Centrifuge to collect precipitate. Renaturalize the precipitate with denaturant such as urea. 5. Centrifuge and collect the supernatant.
Boster Scientific Support
Answered: 2020-03-13
Question
Is EK1440 suitable with potency assay for AAV-induced GAD65 protein in cultured cell?
Verified customer
Asked: 2020-03-11
Answer
Our lab hasn't detected AAV-induced GAD65 protein in cultured cell using GFP ELISA Kit PicoKine EK1440. It is suggested to run a pilot test to see if the kit will work for the specific sample.
Boster Scientific Support
Answered: 2020-03-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-06-29
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-29
Question
Q: if the enzyme conjugated GFP antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GFP antigen?
M. Allen
Verified customer
Asked: 2019-04-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-05
Question
Q: how do I analyze ELISA data? I measured GFP level in cell lysates.
M. Williams
Verified customer
Asked: 2019-03-28
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-03-28
Question
Is there a separate protocol for E.coli cell lysates using EK1440? Or would the protocol on the datasheet be fine?
Verified customer
Asked: 2019-03-07
Answer
The separate sample preparation protocols for E.coli cell lysates using GFP ELISA Kit PicoKine™ (EK1440) are the following: Protein presents as supernatant 1. Centrifuge to collect E.coli. 2. Resuspend E.coli in PBS. 3. Sonicate to break up E.coli. 4. Centrifuge the lysates and collect the supernatant. Protein presents as inclusion body 1. Centrifuge to collect E.coli. 2. Resuspend E.coli in PBS. 3. Sonicate to break up E.coli. 4. Centrifuge to collect precipitate. Renaturalize the precipitate with denaturant such as urea. 5. Centrifuge and collect the supernatant.
Boster Scientific Support
Answered: 2019-03-11
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-27
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Williams
Verified customer
Asked: 2018-02-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-02-11
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-03-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-03-15
