ELOVL5 (NM_021814) Human Over-expression Lysate

Lysate of ELOVL5 over-expression cell lines, WB control

Transient overexpression lysate of ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) (ELOVL5)

Product Info Summary

SKU: LS060481
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) (ELOVL5)

Product Name

ELOVL5 (NM_021814) Human Over-expression Lysate

See all ELOVL5 products

SKU/Catalog Number

LS060481

Size

100 µg

Description

Transient overexpression lysate of ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) (ELOVL5)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

ELOVL5 (NM_021814) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS060481)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for ELOVL5 (Source: Uniprot.org, NCBI)

Gene Name

ELOVL5

Full Name

Elongation of very long chain fatty acids protein 5

Weight

Superfamily

ELO family

Alternative Names

dJ483K16.1; EC 2.3.1.n8; elongation of very long chain fatty acids protein 5; ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2; ELOVL2,3-keto acyl-CoA synthase ELOVL5; Fatty acid elongase 1; hELO1; HELO1homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2; homolog of yeast long chain polyunsaturated fatty acid elongatio; SUR4/Elo3-like, yeast) ELOVL5 HELO1, SCA38, dJ483K16.1 ELOVL fatty acid elongase 5 elongation of very long chain fatty acids protein 5|3-keto acyl-CoA synthase ELOVL5|ELOVL FA elongase 5|ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast)|fatty acid elongase 1|homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2|spinocerebellar ataxia 38|very long chain 3-ketoacyl-CoA synthase 5|very long chain 3-oxoacyl-CoA synthase 5

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on ELOVL5, check out the ELOVL5 Infographic

ELOVL5 infographic

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In this infographic, you will see the following information for ELOVL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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