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ELISA Technical Resources
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Technical Resources Overview
ELISA Principle
Western blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein. ELISAs are much more precise and sensitive than other forms of immunoassay, capable of quantitatively detecting even the most minute concentrations of protein in a sample.
Click Here for MoreELISA Sample Preparation
Every immunoassay protocol begins with sample preparation. Western blot, IHC, and ELISA all require different ways of preparing a sample, and nearly every sample type requires specialized treatment in order to produce high-quality, consistent results. ELISAs can accurately assess soluble proteins in their native state, so are ideal for samples such as urine or saliva. Check out the ELISA sample preparation guides to learn how to get the best results from your sample type.
Click Here for MoreELISA Protocol
The Boster Bio protocol for ELISA offers a step-by-step overview of the procedure. Use this guide as a primer or a quick reference guide, and see our product datasheets or sample preparation guides for more detailed information.
Click Here for MoreELISA Optimization Tips
Nearly every step of the ELISA procedure comes with a choice. Which sample preparation method is best? How concentrated should the antibodies be? Which blocking buffer will work best? The Boster Bio optimization tips are meant to help answer those questions and more. Take a look at our optimization guides to help inform your decisions, optimize your experiments, and get better results.
Click Here for MoreELISA Troubleshooting Tips
Every researcher eventually encounters trouble with an experiment. Blots appear blank, or IHC stains come out blurry, or ELISA standard curves come out wonky, or any of a thousand different problems. Proper controls can help eliminate a great number of possible sources of error, but many troubles will remain. Check out the Boster troubleshooting guides for the most common troubles, their sources, and how to resolve them.
Click Here for MoreELISA Technical Blogs
Here is our ever growing archive of technical blog articles related to ELISA application. These include tips and tricks for experimental optimization, explanations of fundamental principles, more details on the why's and how's of ELISA protocols, related product recommendations, and more. Keep checking back for the most recent topics about ELISA research in the lab, including ELISA optimization!
Click Here for MoreRelated Pages
Flow Cytometry Technical Resource Center
Click Here for More Flow Cytometry Sample Preparation Every flow cytometry (or FACS) experiment begins with sample preparation. Click Here for More Flow Cytometry Technical Blogs Here is our ever growing archive of technical blog articles related to ...
See MoreELISA Data Analysis
After performing an ELISA with a ready-to-use ELISA kit or an antibody pair kit, the data generated must be analyzed to quantitate the target protein concentrations. Below, we discuss the different aspects to consider for more consistent and accurate ELISA data. Furthermore, we provide a step-by-step guide to create the standard curve for analysis...
See MoreImmunohistochemistry (IHC) Resource Center
The aim of these resources is to provide a comprehensive overview of the immunohistochemistry (IHC) resources available for use in research. We have selected a number of key IHC protocols, troubleshootin,g, and optimization tips that are widely used by researchers, as well as some useful tools and techniques that can be applied to specific types of tissue or cells.
See MoreAll Technical Support Resources
Boster provides comprehensive technical resources for applications, blogs, research area/pathway map /disease information, and web-based digital tools.
See MoreELISA Principle, How ELISA Works - Immunoassays
Bosterbio provides a detailed ELISA principle, types of ELISA methods, and a comprehensive ELISA comparison. Learn the concept behind ELISA assay Today!
See MoreWestern Blotting Technical Resource Center
Check out our Western blotting sample preparation resources to learn how to prepare your sample type for Western blotting. Click Here for More Western Blotting Sample Preparation Every immunoassay protocol begins with sample preparation. Click Here f...
See MoreGuidelines for Preparing ELISA Standards
The standard curve is prepared through serial dilutions of the standard with known concentrations that should span the standard curve range. To set up a standard curve, ELISA standards should be carefully prepared for accurate sample quantification o...
See MoreHow to Choose a Good ELISA Kit
If you know your samples contain extremely low levels of the target analyte, you need ELISA kits with high sensitivity for detection (such as Boster's PicokineTM ELISA kits). However, please also be aware of the number of tests the kit provides and...
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