EGF Human, Epidermal Growth Factor Human Recombinant Protein, Pichia

Product Name

EGF Human, Epidermal Growth Factor Human Recombinant Protein, Pichia

View all EGF recombinant proteins

SKU/Catalog Number

PROTQ6QBS2

Size

100ug, 500ug, 1mg

Description

Epidermal Growth Factor Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa. The EGF is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

EGF Human, Epidermal Growth Factor Human Recombinant Protein, Pichia (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ6QBS2)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a filtered concentrated solution in PBS, pH 7.4.

Purity

Greater than 98.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Reconstitution

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by ³H-thymidine uptake) is less than 0.1 ng/ml corresponding to a specific activity of 1 x 10⁷ Units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For EGF (Source: Uniprot.org, NCBI)

Alternative Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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