EGF Human, Epidermal Growth Factor Human Recombinant Protein, Pichia

Product Name

EGF Human, Epidermal Growth Factor Human Recombinant Protein, Pichia

View all EGF recombinant proteins

SKU/Catalog Number

PROTQ6QBS2

Size

100ug, 500ug, 1mg

Description

Epidermal Growth Factor Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa. The EGF is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

EGF Human, Epidermal Growth Factor Human Recombinant Protein, Pichia (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ6QBS2)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a filtered concentrated solution in PBS, pH 7.4.

Purity

Greater than 98.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

133.994kDa

Reconstitution

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by ³H-thymidine uptake) is less than 0.1 ng/ml corresponding to a specific activity of 1 x 10⁷ Units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For EGF (Source: Uniprot.org, NCBI)

Alternative Names

Urogastrone; URG; EGF EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis