Product Info Summary
SKU: | LS079594 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of mitochondrial E3 ubiquitin ligase 1 (MUL1), nuclear gene encoding mitochondrial protein |
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Product info
Product Name
E3 ubiquitin protein ligase MUL1 (MUL1) (NM_024544) Human Over-expression Lysate
SKU/Catalog Number
LS079594
Size
100 µg
Description
Transient overexpression lysate of mitochondrial E3 ubiquitin ligase 1 (MUL1), nuclear gene encoding mitochondrial protein
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
E3 ubiquitin protein ligase MUL1 (MUL1) (NM_024544) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS079594)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
![ls079594 e3 ubiquitin protein ligase mul1 mul1 human over expression lysates nm 024544 wb testing 1 ls079594 e3 ubiquitin protein ligase mul1 mul1 human over expression lysates nm 024544 wb testing 1](https://www.bosterbio.com/media/catalog/product/l/s/ls079594-e3-ubiquitin-protein-ligase-mul1-mul1-human-over-expression-lysates-nm-024544-wb-testing-1.jpg)
Click image to see more details
E3 ubiquitin protein ligase MUL1 (MUL1) Human Over-expression Lysates NM_024544.
Western blot validation of overexpression lysate (LS079594) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MUL1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Mul1 (Source: Uniprot.org, NCBI)
Gene Name
Mul1
Full Name
Mitochondrial ubiquitin ligase activator of NFKB 1
Weight
Alternative Names
C1orf166mitochondrial E3 ubiquitin ligase 1; E3 ubiquitin-protein ligase MUL1; EC 6.3.2; EC 6.3.2.-; FLJ12875; GIDERP11-401M16.2; Growth inhibition and death E3 ligase; MAPLE3 ubiquitin ligase; mitochondrial E3 ubiquitin protein ligase 1; mitochondrial ubiquitin ligase activator of NFKB 1; mitochondrial ubiquitin ligase activator of NF-kB; Mitochondrial-anchored protein ligase; MULANchromosome 1 open reading frame 166; Putative NF-kappa-B-activating protein 266; RING finger protein 218; RNF218mitochondria-anchored protein ligase Mul1|0610009K11Rik, AV000801, Gide|mitochondrial ubiquitin ligase activator of NFKB 1|mitochondrial ubiquitin ligase activator of NFKB 1|E3 ubiquitin ligase|E3 ubiquitin-protein ligase MUL1|RING-type E3 ubiquitin transferase NFKB 1|growth inhibition and death E3 ligase|protein Hades
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Mul1, check out the Mul1 Infographic
![Mul1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mul1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For E3 ubiquitin protein ligase MUL1 (MUL1) (NM_024544) Human Over-expression Lysate (LS079594)
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