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Product Info Summary
| SKU: | LS084444 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) |
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Product info
Product Name
DOT1L (NM_032482) Human Over-expression Lysate
SKU/Catalog Number
LS084444
Size
100 µg
Description
Transient overexpression lysate of DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
DOT1L (NM_032482) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084444)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DOT1L Human Over-expression Lysates NM_032482.
Western blot validation of overexpression lysate (LS084444) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DOT1L (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for DOT1L (Source: Uniprot.org, NCBI)
Gene Name
DOT1L
Full Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Weight
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
DOT1L; DOT1-like protein; DOT1-like, histone H3 methyltransferase (S. cerevisiae); EC 2.1.1.43; H3-K79-HMTase; KIAA1814DKFZp586P1823; KMT4; KMT4H3 lysine-79 specific; Lysine N-methyltransferase 4 DOT1L DOT1, KMT4 DOT1 like histone lysine methyltransferase histone-lysine N-methyltransferase, H3 lysine-79 specific|DOT1 like histone H3K79 methyltransferase|DOT1-like histone methyltransferase|DOT1-like protein|DOT1-like, histone H3 methyltransferase|H3-K79-HMTase|histone H3-K79 methyltransferase|histone methyltransferase DOT1L|lysine N-methyltransferase 4
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DOT1L, check out the DOT1L Infographic
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In this infographic, you will see the following information for DOT1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DOT1L (NM_032482) Human Over-expression Lysate (LS084444)
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