Click image to see more details
Product Info Summary
| SKU: | EKC1694 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
Customers Who Bought This Also Bought
Product info
Product Name
DGKA Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1694
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The DGKA Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DGKA protein expression profile in cells. The kit can be used for measuring the relative amounts of DGKA in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DGKA.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
DGKA Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1694)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1694 is reactive to DGKA in Human samples
Application Guarantee
EKC1694 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-DGKA Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
Click image to see more details
Western blot analysis of extracts from Jurkat cells
Protein Target Info & Infographic
Gene/Protein Information For DGKA (Source: Uniprot.Org, NCBI)
Gene Name
DGKA
Full Name
Diacylglycerol kinase alpha
Weight
82630 MW
Superfamily
eukaryotic diacylglycerol kinase family
Alternative Names
Diacylglycerol kinase alpha;DAG kinase alpha;2.7.1.107;80 kDa diacylglycerol kinase;Diglyceride kinase alpha;DGK-alpha;DGKA;DAGK, DAGK1; DGKA DAGK, DAGK1, DGK-alpha diacylglycerol kinase alpha diacylglycerol kinase alpha|80 kDa diacylglycerol kinase|DAG kinase alpha|diacylglycerol kinase, alpha 80kDa|diglyceride kinase alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DGKA, check out the DGKA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DGKA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DGKA Colorimetric Cell-Based ELISA (EKC1694)
Hello CJ!
No publications found for EKC1694
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used DGKA Colorimetric Cell-Based ELISA?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For DGKA Colorimetric Cell-Based ELISA
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
