AffiniPure Mouse Anti-Rabbit IgG (H+L) secondary antibody, TRITC Conjugated

Product Info Summary

SKU: BA1033
Size: 0.5 ml
Reactive Species: Rabbit
Host: E. coli
Application: Flow Cytometry, IF

SKUBA1033
ApplicationsIF, FCM
Pack size0.5ml
HostE. coli
Concentration1 mg/ml
SpecificityThis CY3 conjugated Protein A, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1.
StorageAt -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protected from light.
Purity>99%
Contents1 mg/ml of CY3 conjugated specific antibody; 0.01M PBS, 50% glycerol.
FluorophoreAmax=554nm; Emax=568nm
FormConcentrated, Liquid
ConjugationCy3

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunofluorescence, 1.25-5 μg/mL
Flow Cytometry, 1.25-5 μg/mL

Validation Images & Assay Conditions

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Total: $150

SKU:BA1033

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