CNOT2 Colorimetric Cell-Based ELISA Kit

CNOT2 ELISA kit, cell-based

The CNOT2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CNOT2 protein expression profile in cells. The kit can be used for measuring the relative amounts of CNOT2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CNOT2.

Product Info Summary

SKU: EKC1125
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

CNOT2 Colorimetric Cell-Based ELISA Kit

See all CNOT2 products

SKU/Catalog Number

EKC1125

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The CNOT2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CNOT2 protein expression profile in cells. The kit can be used for measuring the relative amounts of CNOT2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CNOT2.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

CNOT2 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1125)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1125 is reactive to CNOT2 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1125 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-CNOT2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For CNOT2 (Source: Uniprot.Org, NCBI)

Gene Name

CNOT2

Full Name

CCR4-NOT transcription complex subunit 2

Weight

59738 MW

Superfamily

CNOT2/3/5 family

Alternative Names

CCR4-associated factor 2;CCR4-NOT transcription complex subunit 2;CCR4-NOT transcription complex, subunit 2;CDC36;CNOT2;HSPC131;MSTP046;NOT2 (negative regulator of transcription 2, yeast) homolog;NOT2H CNOT2 CDC36, HSPC131, IDNADFS, NOT2, NOT2H CCR4-NOT transcription complex subunit 2 CCR4-NOT transcription complex subunit 2|CCR4-associated factor 2|negative regulator of transcription 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CNOT2, check out the CNOT2 Infographic

CNOT2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CNOT2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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