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Product Info Summary
| SKU: | EKC1117 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Claudin 10 Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1117
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The Claudin 10 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Claudin 10 protein expression profile in cells. The kit can be used for measuring the relative amounts of Claudin 10 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Claudin 10.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Claudin 10 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1117)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1117 is reactive to CLDN10 in Human, Mouse samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1117 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Claudin 10 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from LOVO cells
Protein Target Info & Infographic
Gene/Protein Information For CLDN10 (Source: Uniprot.Org, NCBI)
Gene Name
CLDN10
Full Name
Claudin-10
Weight
24488 MW
Superfamily
claudin family
Alternative Names
Claudin-10;OSP-like protein;CLDN10 CLDN10 CPETRL3, HELIX, OSP-L, OSPL claudin 10 claudin-10|OSP-like protein|oligodendrocyte-specific protein-like
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CLDN10, check out the CLDN10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CLDN10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Claudin 10 Colorimetric Cell-Based ELISA Kit (EKC1117)
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