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Product Info Summary
| SKU: | LS440193 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of coiled-coil domain containing 88C (CCDC88C) |
Product info
Product Name
CCDC88C (NM_001080414) Human Over-expression Lysate
SKU/Catalog Number
LS440193
Size
100 µg
Description
Transient overexpression lysate of coiled-coil domain containing 88C (CCDC88C)
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
CCDC88C (NM_001080414) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS440193)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CCDC88C Human Over-expression Lysates NM_001080414.
Western blot validation of overexpression lysate (LS440193) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CCDC88C (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CCDC88C (Source: Uniprot.org, NCBI)
Gene Name
CCDC88C
Full Name
Protein Daple
Weight
226.535kDa
Superfamily
CCDC88 family
Alternative Names
Protein Daple Ccdc88c|0610010D24Rik, AV000935, AW324073, Da, Daple, mKIAA1509|coiled-coil domain containing 88C|protein Daple|DVL-binding protein DAPLE|coiled-coil domain-containing protein 88C|dvl-associating protein with a high frequency of leucine residues
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CCDC88C, check out the CCDC88C Infographic
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In this infographic, you will see the following information for CCDC88C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CCDC88C (NM_001080414) Human Over-expression Lysate (LS440193)
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