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Product Info Summary
| SKU: | EKC1086 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Caspase 3 Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1086
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The Caspase 3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Caspase 3 protein expression profile in cells. The kit can be used for measuring the relative amounts of Caspase 3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caspase 3.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Caspase 3 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1086)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1086 is reactive to CASP3 in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1086 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Caspase 3 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from NIH-3T3 cells
Protein Target Info & Infographic
Gene/Protein Information For CASP3 (Source: Uniprot.Org, NCBI)
Gene Name
CASP3
Full Name
Caspase-3
Weight
31608 MW
Superfamily
peptidase C14A family
Alternative Names
Apopain precursor;CASP-3;CPP-32;CPP32;Caspase-3;Cysteine protease CPP32;ICE3;SCA-1;SREBP cleavage activity 1;Yama protein CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CASP3, check out the CASP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CASP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Caspase 3 Colorimetric Cell-Based ELISA Kit (EKC1086)
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Customer Q&As
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3 Customer Q&As for Caspase 3 Colorimetric Cell-Based ELISA Kit
Question
What is your protocol for EKC1086 based on serum test?
Verified customer
Asked: 2020-01-09
Answer
The following is the protocol for Caspase 3 Colorimetric Cell-Based ELISA Kit EKC1086 used on serum: 1) Collect whole blood into a tube without additives. 2) Keep the blood at room temperature for 30 min. 3) Centrifuge at 3,000 rpm and 4°C for 10 min. 4) Assay immediately or aliquot supernatant (serum) and hold at -80°C (Avoid freeze/thaw cycles). Note: Hemolysis should be avoided while collecting serum samples. Those samples that have undergone hemolysis may increase non-specifically staining in HRP-conjugated ELISA assay.
Boster Scientific Support
Answered: 2020-01-08
Question
Can you confirm if EKC1161 and EKC1086 ELISA kits can be used for serum/plasma samples?
Verified customer
Asked: 2019-12-30
Answer
The Cytochrome C Colorimetric Cell-Based ELISA Kit (EKC1161) and Caspase 3 Colorimetric Cell-Based ELISA Kit (EKC1086) are specifically geared towards whole cells (adherent or suspension) seeded to the bottom of the plate. It is not intended to be used with cell lysates or any other types of samples.
Boster Scientific Support
Answered: 2020-01-02
Question
Can frozen tissue extracts be used as a sample for EKC1086 and EKC1161? What should be the weight or volume of sample?
Verified customer
Asked: 2019-06-10
Answer
Our cell-based ELISA kits are meant to be used with cells being plated, grown, then fixed on the wells. They are not meant to be used with cell lysates or tissue homogenates, as they have not been validated for that purpose. We cannot offer any sort of guarantee using it on tissue samples.
Boster Scientific Support
Answered: 2019-06-13
