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Product Info Summary
| SKU: | EKC2283 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
Calsenilin/KCNIP3 (Phospho-Ser63) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2283
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Calsenilin/KCNIP3 (Phospho-Ser63) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Calsenilin/KCNIP3 (Phospho-Ser63) protein expression profile in cells. The kit can be used for measuring the relative amounts of Calsenilin/KCNIP3 (Phospho-Ser63) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Calsenilin/KCNIP3 (Phospho-Ser63).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Calsenilin/KCNIP3 (Phospho-Ser63) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2283)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2283 is reactive to KCNIP3 in Human, Mouse samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2283 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Calsenilin/KCNIP3 Phospho-Ser63 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Calsenilin/KCNIP3 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from K562 cells treated with forskolin 40nM 30' and 293 cells treated with PMA 125ng/ml 30'
Protein Target Info & Infographic
Gene/Protein Information For KCNIP3 (Source: Uniprot.Org, NCBI)
Gene Name
KCNIP3
Full Name
Calsenilin
Weight
29231 MW
Superfamily
recoverin family
Alternative Names
A-type potassium channel modulatory protein 3;CSEN;DRE-antagonist modulator;DREAM;KCNIP3;KChIP3;Kv channel-interacting protein 3 KCNIP3 CSEN, DREAM, KCHIP3 potassium voltage-gated channel interacting protein 3 calsenilin|A-type potassium channel modulatory protein 3|DRE-antagonist modulator|Kv channel interacting protein 3, calsenilin|calsenilin, presenilin-binding protein, EF hand transcription factor|kv channel-interacting protein 3|potassium channel interacting protein 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on KCNIP3, check out the KCNIP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KCNIP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Calsenilin/KCNIP3 (Phospho-Ser63) Colorimetric Cell-Based ELISA Kit (EKC2283)
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