Kinesin-like protein KIF7 Antibodies

Kinesin-like protein KIF7 (Kif7)

Essential for hedgehog signaling regulation: acts as both a positive and negative regulator of sonic hedgehog (Shh) and Indian hedgehog (Ihh) pathways, acting downstream of SMO, through both SUFU-dependent and -independent mechanisms. Involved in the regulation of microtubular dynamics.

Required for proper organization of the ciliary suggestion and control of ciliary localization of SUFU-GLI2 complexes. Required for localization of GLI3 to cilia in response to Shh.

Gene Information

Mouse
Gene Name:	Kif7
Uniprot:	B7ZNG0
Entrez:	16576

Family

Belongs to:
TRAFAC class myosin-kinesin ATPase superfamily

Alternative Names

EQYK340; kinesin family member 7; kinesin-like protein KIF7; MGC120653; MGC138476; MGC138478; UNQ340

Molecular Weight

Mass (kDA):
151.624 kDA

Genomic Context

Mouse
Location:	7|7 D2
Sequence:	7;

Tissue Specificity

Expressed in heart, lung, liver, kidney, testis, spleen and cerebellum.

Diseases

• Malignant Neoplasms
• Neoplasms
• Acrocallosal Syndrome
• Malignant Neoplasm Of Pancreas
• Cell Transformation, Neoplastic
• Malignant Neoplasm Of Stomach
• Carcinogenesis
• Carcinoma
• Basal Cell Carcinoma
• Colorectal Cancer
• Stomach Neoplasms
• Familial Aplasia Of The Vermis

• Dysplasia
• Congenital Absence
• Congenital Abnormality
• Polydactyly
• Hypoplasia
• Inflammation
• Melanoma

Pathways

• Localization
• Cell Proliferation
• Proteolysis
• Regeneration
• Tissue Homeostasis
• Transport
• Hair Follicle Development
• Aging
• Stem Cell Maintenance
• Skin Development
• Intracellular Signal Transduction
• Endocytosis

• Chondrocyte Proliferation
• Cell Fate Specification
• Tissue Regeneration
• Chondrocyte Development
• Cell Cycle
• Intraflagellar Transport
• Rna Interference

PTMs

• Phosphorylation
• Cleavage

• Acetylation
• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Kif7

Boster Bio: Best Uses of the KIF7 Marker

If you're interested in using KIF7 in your studies, then you've come to the right place. This article will discuss its use in biochemistry and pharmacy. It also discusses the importance and use of membrane staining in protein transfer, Autoradiography films, and DAB-chromogenic detection methods. Boster Bio, Best Uses and the KIF7Marker summarizes the most recent research regarding this protein.

Protein transfer efficiency by membrane staining

A pooled sample containing 10mg of human serum was separated by SDS–PAGE and transferred onto PVDF membranes or nitrocellulose membranes. These membranes were then cut into five equal parts and fixed with acetone or 50% methanol. Subsequently, the samples were stained with LCA and SNA lectins. These samples were heated up to 100°C for 30 seconds, and then analyzed quantitatively.

The proteins were transferred from NC to PVDF membranes with an 8% SDS–PAGE gradient. The bound proteins were stained using direct blue-71. The band intensities of the bound proteins were quantified with GraphPad Prism version 6.

The sample comb should then be gently pressed onto the membrane to detect proteins. After pressing the sample comb carefully over the membrane, it should be incubated at a temperature of 37°C for between 30-60 minutes. It may be necessary to optimize the time of incubation. To remove any unbound antibodies, the membrane should first be washed three more times with TBS Wash Buffer. A secondary antibody should be added in a ratio of 3:1 to the membrane after it has been washed.

To quantify the transfer efficiency of proteins, the blots were incubated with antibodies, lectins, or proteins. To visualize the proteins bands, the Anti-mouse IgG MAb was used. Membranes were then set in a Canon scanner. The resulting images were then analyzed using densitometry. Images were then analyzed using Image J and GraphPad Prism version 6.

Film about autoradiography

This marker has many applications. If you're not familiar with its uses, it is easy for you to find one. We will be discussing its use in biomedical science. This protein is a receptor of kinesin which is a nucleoprotein that controls the expression and function of several other genes. The marker is used to identify these genes, and it is derived from bovine chorionic gonadotropin (hs-Ca2+).

ECL chemiluminescent detection system

The ECL chemical chemiluminescent detection is a widely-used method for quantitatively copying proteins. It has some limitations. It is not possible for both enzymatic processes to be captured simultaneously. Multiplex PCR cannot be performed simultaneously or in every run. It is also impossible to quantify the amount of protein on a membrane. Researchers must therefore learn more about ECL limitations and how they can be overcome.

The binding of a primary antibody and a target protein is the first step in ECL chemistry. The secondary antibody is usually labeled with an enzyme (alkaline phosphatase and horseradish oxidase), which releases photons if it reacts with the target proteins. You can measure the photons produced by the reaction using either an xray film or digital imaging.

Enhanced chemiluminescence, a luminol based substrate, can be used for Western Blot applications. The amount of antigen in the substrate is proportional to the reaction between HRP-labeled antibody and the substrate produces a luminous signal. This indirect method of protein detection is further enhanced by the use of pictograms.

The first step is to prepare a high salt wash buffer containing 10 mM Tris (pH 7.4), 0.5 M NaCl and 1 mM NaH2PO4 with 0.1% SDS/Tween-20. The next step is to blot the membrane with the protein side facing up. The blot membrane should be placed on the film and exposed for one minute. Light emission is at its peak within five to thirty minutes of incubation of the substrate. Finally, a CCD detector is used to capture chemiluminescent images.

DAB chromogenic identification system

Boster Bio's DAB system for chromogenic detection is a powerful system. It integrates a unique technology called Intensification that uses phosphors to enhance chromogenic signals. This system uses horseradish peroxidase to catalyze deposition of two separate Linkers. These Linkers react with streptavidin-peroxidase conjugates, and then detect proteins using diaminobenzidine (DAB).