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- Table of Contents
Facts about WW domain-containing adapter protein with coiled-coil.
Positive regulator of amino acid starvation- induced autophagy (PubMed:22354037). Also acts as a negative regulator of basal autophagy (PubMed:26812014).
Human | |
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Gene Name: | WAC |
Uniprot: | Q9BTA9 |
Entrez: | 51322 |
Belongs to: |
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No superfamily |
1110067P07Rik; bA48B24; bA48B24.1; BM-016; FLJ31290; KIAA1844; MGC10753; PRO1741; WW domain containing adaptor with coiled-coil; WW domain-containing adapter protein with coiled-coil; WW domain-containing adaptor with coiled coil; Wwp4
Mass (kDA):
70.724 kDA
Human | |
---|---|
Location: | 10p12.1|10p12.1-p11.2 |
Sequence: | 10; NC_000010.11 (28532772..28623112) |
Nucleus speckle. Nucleus. In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes.
If you are in need of high-affinity primary antibodies, you should consider using the anti-WAC Marker from Boster Bio. This product is used to detect peroxidase and is applicable to scientists worldwide. You can submit your results for species, applications, and special samples using Boster Bio. Additionally, it is applicable to all scientists from all fields. You can learn more about Boster Bio and WAC Markers by reading this article.
If you're in the market for high-affinity primary antibodies, then you've come to the right place. Boster Bio has more than 16,000 antibodies available in its catalog for research use. These antibodies have been extensively validated in ELISA, WB, and IHC applications. The company offers rabbit polyclonal antibodies, as well as mouse and human ELISA kits. Using these antibodies in your research is as simple as ordering a reagent. And all Boster Bio products come with a high-quality guarantee that they'll perform as advertised.
If you're interested in obtaining antibodies to perform flow cytometry, then you've come to the right place. Boster Bio offers high-affinity primary antibodies that are both monoclonal and polyclonal. These antibodies are widely used in research and have been cited over the last 25 years. They're also available through customized services and BeNeLux deliveries. Contact Boster Bio today for more information on their products and services.
Using a monoclonal antibody as a reference for research purposes can be a helpful option in evaluating new drug candidates. For example, h5E12-L230G is a high-affinity anti-PCSK9 antibody. Because it exhibits an extremely slow dissociation rate, it may be useful in treating hypercholesterolemia and cardiovascular diseases. The company's high-affinity primary antibodies are derived from a variety of sources, including a number of biotech companies.
When it comes to primary antibodies, quality is often determined by the specificity and affinity of the antibody. Higher affinity means a higher likelihood that the antibody will recognize the antigen of interest, while lower specificity means a higher probability of binding to unintended antigens. High-affinity antibodies are valuable tools for measuring, purifying, and measuring an antigen of interest. But in order to maximize their effectiveness, primary antibodies must meet specific criteria.
Other companies producing high-affinity primary antibodies include Diatec, Exonbio, and Gallus Immunotech. These companies specialize in producing both polyclonal antibodies and monoclonal antibodies. Diatec produces polyclonal antibodies and monoclonal antibodies in chickens. The latter company also provides high-affinity antibodies using modified proteins. Finally, Biogenes offers contract research and development services.
The Boster Bio Anti-WAC Marker is an antibody that reacts with Human Cytokeratin (WAC). This non-hazardous, human-specific antibody is available without BSA. Blocking peptide is also available, which can be purchased at 1.0 mg/ml. The price will vary depending on the immunogen length. The antibody is tested against known positive and negative samples to ensure that it will work in your experiment.
DAB is used to detect peroxidase in tissue sections. The most common substrate for this assay is diaminobenzidine. This compound is insoluble in both alcohol and water. It is compatible with many common histological stains. Metal salts can be added to the DAB solution to increase its sensitivity. A variety of chromogens are available in the market.
The three-dimensional DAB technique was developed by Graham and Karnovsky to demonstrate the cytochemical localization of horseradish peroxidase. Other proteins and enzymes with peroxidase activity were subsequently demonstrated by modifying the DAB procedure. However, this method has not been particularly specific. Peroxisomal catalase has various fixation and incubation requirements, so the technique is not completely applicable. However, researchers such as Fahimi and Herzog analyzed the requisites for the selective demonstration of calase and peroxidase.
DAB was developed by Graham and Karnovsky in 1966. This compound is now used widely in transmission electron microscopy and optical microscopy to detect peroxidase activity. It converts into osmium black when subjected to peroxide in cells. This process also allows researchers to visualize how peroxidase activity affects cell growth. The sensitivity of these techniques was further increased by adjusting the parameters of the enzyme reaction.
DAB staining is a type of immunohistochemical staining. DAB staining has both advantages and disadvantages. There are several types of DAB staining reagents, including biotin-conjugated secondary antibodies and streptavidin-HRP conjugates. For double labeling in IHC/ISH, DAB staining is particularly useful, as the slide can remain stained for several years.
A further development of the indirect method is the PAP method. This technique uses a rabbit antibody that reacts with peroxidase as a third layer antigen. The rabbit antibody is then bound to a goat anti-rabbit gaba-globulin antibody. This method reduces background staining and allows for a higher dilution of the primary antibody.
HRP conjugates are commonly used in indirect assays. These enzymes are not light-sensitive. Instead, they bind to a molecule bound to the target, and the signal is generated. This indirect method has the advantage of being more sensitive than direct as there are no specificities for the target. It also saves time. There are several advantages to using HRP conjugates.
PMID: 22354037 by McKnight N.C., et al. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC.
PMID: 25356899 by Hamdan F.F., et al. De novo mutations in moderate or severe intellectual disability.