UCP3 Antibodies

Mitochondrial uncoupling protein 3 (UCP3)

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the kind of heat.

May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.

Gene Information

Human
Gene Name:	UCP3
Uniprot:	P55916
Entrez:	7352

Family

Belongs to:
mitochondrial carrier (TC 2.A.29) family

Alternative Names

mitochondrial uncoupling protein 3; SLC25A9Solute carrier family 25 member 9; UCP 3; uncoupling protein 3 (mitochondrial, proton carrier); Uncoupling protein-3

Molecular Weight

Mass (kDA):
34.216 kDA

Genomic Context

Human
Location:	11q13.4
Sequence:	11; NC_000011.10 (74000277..74009237, complement)

Tissue Specificity

Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.

Subcellular Localizations

Mitochondrion inner membrane; Multi-pass membrane protein.

Diseases

• Obesity
• Diabetes Mellitus
• Diabetes Mellitus, Non-insulin-dependent
• Insulin Resistance
• Insulin Sensitivity
• Nervousness
• Malignant Neoplasms
• Neoplasms
• Inflammation
• Dysequilibrium Syndrome
• Cardiovascular Diseases
• Metabolic Diseases

• Diabetes Mellitus, Experimental
• Hyperinsulinism
• Cachexia
• Hyperglycemia
• Hypothyroidism
• Hyperthyroidism

Pathways

• Transport
• Oxidative Phosphorylation
• Fatty Acid Oxidation
• Secretion
• Energy Homeostasis
• Aging
• Insulin Secretion
• Localization
• Lipid Oxidation
• Electron Transport
• Pathogenesis
• Proton Transport
• Electron Transport Chain

• Response To Cold
• Glycolysis
• Fatty Acid Transport
• Swimming
• Tricarboxylic Acid Cycle
• Uniport
• Adp Phosphorylation

PTMs

• Oxidation
• Phosphorylation
• Cleavage
• Glutathionylation
• Acetylation
• Sumoylation
• Carboxylation

• Dephosphorylation
• Deglutathionylation
• Ribosylation
• Nitration
• Reduction
• Deacetylation
• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/UCP3

The Boster Bio: Best Uses Of The UCP3 Marker

The Boster bio: Best Uses For The Universal Cell Signaling Protein UCP3M As a biotechnologist and a biotechnologist, I have used many different markers to see how the body responds. This marker is composed of two subtypes, UCP2 or UCP3. This article will describe the differences between the two subtypes and give you ideas on how to use the markers.

UCP2

Researchers have recently reported that UCP2 a protein plays a critical role in sepsis. The enzyme plays a role in metabolism and regulates ROS levels. Moreover, it is also a critical mediator in sepsis. UCP2 might be beneficial in the survival of cancer cell lines. In this context, researchers have identified UCP2 as a potential target of Boster Bio.

The study revealed that UCP2-/BMDMs were significantly less likely to induce FASN and lipid synthesis. However, GLUT1 expression was very similar. These results suggest that UCP2 might play a role in the regulation of glucose metabolism in macrophages. UCP2 also regulates lipid production by modulating glycolysis. Thus, UCP2 is essential for the regulation of de novo lipid synthesis in macrophages.

In the future, the research should address questions that are both theoretical and practical. In the near future, a new drug targeting UCP2 may be developed that could be used to treat anemia. However, more studies are necessary to determine the mechanism of action of UCP2.

It is well-known that UCP2 has antioxidant properties. This effect has been shown in a variety of experimental systems, including cancer cells. UCP2's effectiveness as an anti-cancer drug has been confirmed by genetic and pharmacological ablation. UCP2's antioxidant ability may be due to its ability to protect cells against oxidative damage. UCP2 may also have an antioxidant role, which is related to its ability to detect mitochondrial ROS. However, this sensor has a greater functional effect when it is exposed to oxidative Stress.

UCP3

Identifying the UCP3 marker in cells is important in various diagnostic procedures. The gene encoding the UCP3 protein is widely expressed throughout the human body. It is essential for diagnosing many diseases accurately. This marker is used to identify abnormal cell growth. In a study of breast carcinoma cells, the UCP3 protein can be detected using the UCP3 (D6J8K), Rabbit mAb.

The UCP3 gene, an inner membrane transport protein, acts on mitochondria. It regulates glucose transport and decreases the production reactive oxygen species in mitochondria. As a result, UCP3 is believed to protect against the development of insulin resistance and fat gain in humans. However, the use of UCP3 for this purpose is questionable. Fortunately, there have been many studies that have examined the role of UCP3 during various diseases.

Researchers conducted a study with obese and non-obese subjects to determine if the UCP3 gene was involved in fat storage. The study used HEK293 cells that had been transiently transfected with wt or mutant UCP3 plasmids in a 1:1 ratio. The cells were transfected with 500 or 1 mg palmitate. The resulting oil-red O staining revealed that the UCP3L was significantly reduced after transfection.

UCP4

You have found the right place to find an antibody that recognizes UCP4. This product, catalogue number A09782, has been approved for use in WB applications. It is tested for human and can be stored at -20degC for one year. It reacts with a partial length recombinant UCP4 protein. Blocking peptides are available to help you perform WB tests. This antibody can be used in combination with positive or negative samples.

Researchers have shown that UCPs can regulate redox in cells. UCPs not only protect against oxidative stress but also promote mitochondrial proton loss. In a mouse model for Parkinson's disease, decreased UCP4 expression decreases hepatocytes’ cold tolerance. This study also implicated UCP4 in redox regulatory. It is not yet clear how the protein protects mitochondria in oxidative stress.

UCPs can be used to protect insects from the cold. Cold-sensitive insects build up cryoprotectants in hemolymph and change their membrane composition in response to cold. Insect mitochondria are not fully understood, but UCPs may play a role in controlling calcium homeostasis and the apoptotic process caused by cold. Thus, UCP has a thermogenic role in insects, and may play a vital role in the stress tolerance of these animals.

UCP5

The UCP3 receptor is a protein which regulates the expression and function of a number other proteins. Its expression depends upon the presence or absence of the UCP1 Protein. The expression level of the UCP3 gene has been studied in rat and mouse. Hilse, et al. claim that UCP3 only occurs in a fraction of cells in human bodies. Its expression levels are much lower that those of UCP2 & UCP4.

Its transport activity has been demonstrated in biomimetic system, such as liposomes. Reconstituted UCP3 transports hydrogen at a rate greater than 2.6 s-1 when it is surrounded by FA. This is more than five-times faster than UCP1 transport rates which range from one up 700 s-1. However, its exact biological function is still unknown.

The protein is found in a limited amount of tissues and is thought have an anti-oxidative function. Exercise has also been shown to increase the levels of UCP3 and is related to exercise. This hypothesis needs to be tested further. Further experiments will be needed to confirm if the UCP3 short-chain isoform can inhibit UCP1.

The UCP3 markers have many other uses. It has been used as a marker for mitochondrial carriers. However, its involvement in ROS regulation is doubtful. Other mitochondrial carriers, such as ANT, can mediate mild uncoupling. This makes it difficult, if not impossible, to develop antibodies that target the protein. The UCP3 protein is a promising tool in research.

UCP6

The best uses of UCP3 markers for proton transportation and regulatory proteins are still unknown. UCP1 & UCP3 are identical in structure and function but differ in molecular mechanisms. UCP3 is associated with FA boxidation in cell metabolism. This indicates that UCP3 might transport a substrate essential for FA B-oxidation.

Although UCP3 has no significant uncoupling function in skeletal muscle, it is thought to transport superoxide anions, a cellular defense against ROS. Exercise is a known contributor to increased UCP3 levels, and it is therefore useful for monitoring muscle protein synthesis and depletion. The best uses of the UCP3 marker can be found in clinical research for athletes and for assessing the benefits of endurance exercise.

Although the best uses of UCP3 markers have not been determined, several studies have shown that the enzyme plays a significant role in the regulation of BAT activity. In order to regulate the release nitric dioxide, a functional BAT must contain UCP3. Taking it out of the cell will cause this process to be inhibited. The activation of the BAT is also dependent on the UCP3 transport function.

Overexpression studies show that UCP3 markers are preferentially expressed at the heart and in skeletal muscle. However, it is not clear what role the protein plays in these tissues. UCP1 (highly abundant) and UCP3 (lowly abundant) make it unclear whether UCP3 plays a role in BAT. Therefore, it is important to study the UCP3 Marker for its most effective uses in cell biology.

UCP7

The UCP3 marker, which is a protein found in nearly all eukaryotic tissues, is also present. Its expression levels vary from their UCP1 counterparts by as much as 1,000 fold. UCP3's proton conductance is lower than UCP1. Listed below are the Best Uses Of The UCP3 Marker

The UCP3 protein is present in all cells. It plays a key role in fat oxidation. It is also known to reduce the production of oxidative stress in neurons and reduce fatty acid anions. Numerous studies have shown that the UCP3 gene plays an important role in fat metabolism, as well as in diabetes and cardiovascular disease. Hence, it is important to identify genetic variants in order to determine how human populations respond to diets and exercise.

One study showed that UCP3 stimulates thermogenesis in skeletal muscles. It was surprising that this protein didn't affect heat production. This protein also plays a role in exporting fatty acids outside the mitochondrial matrix to tissues. It is not known if this protein plays a significant function in energy regulation. It is important not to forget that the UCP3 protein marker is only one of many tools available in the area of energy regulation.

In addition, UCP3 has many other functions. It is found in skeletal muscles and acts as a defense against ROS. Exercise also increases UCP3 protein. This protein may increase in the mitochondria and help with the treatment or prevention of cardiovascular disease. It is vital for human health. It is vital for research into the biology and occurrences of diabetes.