Toll-like receptor 4 Antibodies

AND TLR4 ELISA kits, Toll-like receptor 4 Proteins

Many biological assays use antibodies to detect Toll-like receptor 4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Toll-like receptor 4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Toll-like receptor 4 antibodies...

We validate the specificity of these antibodies to Toll-like receptor 4 by testing them on tissues known to express TLR4 positively and negatively. Browse below to find the TLR4 antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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TLR4 Infographic by Boster Bio

All TLR4 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-Toll-like receptor 4 TLR4 Antibody Picoband®

30 Citations 5 Q&As

Human

IHC, WB

$370
Cat # PA1484
100 μg/vial

Anti-Toll-like Receptor 4 TLR4 Antibody

12 Citations 16 Q&As

Human, Mouse

IF, IHC

$805
Cat # A00017
200µg

Anti-TLR4 (S441) Antibody

4 Citations

Human, Mouse, Rat

IP, IF, IHC, WB

$405
Cat # A00017S441
100ul

Anti-TLR4 Antibody Picoband®

Human

ELISA, Flow Cytometry, WB

$370
Cat # A00017-2
100 μg/vial

Anti-Tlr4 Antibody Picoband®

Mouse

ELISA, WB

$370
Cat # A00017-3
100 μg/vial

TLR4 (NM_138554) Human Over-expression Lysate

Human

$960
Cat # LS007099
100 µg

TLR4 Overview

Facts about Toll-like receptor 4.

TLR4 3D structure. Source: alphafold

Toll-like receptor 4 (TLR4)

Acts via MYD88, TIRAP and TRAF6, leading to NF- kappa-B activation, cytokine secretion and the inflammatory reaction (PubMed:9237759, PubMed:10835634, PubMed:27022195). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+).

Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific (PubMed:20711192). Both M.

Gene Information

Human
Gene Name:	TLR4
Uniprot:	O00206
Entrez:	7099

Family

Belongs to:

Toll-like receptor family

Alternative Names

76B357.1; ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; tlr4 76B357.1; TLR4 facs; TLR4 flow cytometry; TLR4 human; TLR4 ihc; TLR4 western blot; TLR4; TOLL; toll-like receptor 4

Molecular Weight

Mass (kDA):
95.68 kDA

Genomic Context


Human

Location:	9q33.1
Sequence:	9; NC_000009.12 (117704403..117724735)

Tissue Specificity

Highly expressed in placenta, spleen and peripheral blood leukocytes (PubMed:9435236, PubMed:9237759). Detected in monocytes, macrophages, dendritic cells and several types of T-cells (PubMed:9237759, PubMed:27022195).

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Early endosome. Cell projection, ruffle. Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis (PubMed:20037584). Colocalizes with RFTN1 at cell membrane and then together with RFTN1 moves to endosomes, upon lipopolysaccharide stimulation.

Diseases

Inflammation
Innate Immune Response
Inflammatory Response
Infective Disorder
Systemic Infection
Malignant Neoplasms
Bacterial Infections
Pneumonia
Neoplasms
Atherosclerosis
Tissue Adhesions
Autoimmune Reaction
Ischemia

Colitis
Salmonella Infections
Reperfusion Injury
Septic Shock
Asthma
Arthritis
Obesity

Interacting Proteins

LY96
MYD88
NOX4
SIGLEC5
SIGLEC9

TICAM2
TIRAP
TLR6
TNC

Pathways

Immune Response
Secretion
Inflammatory Response
Cytokine Production
Pathogenesis
Innate Immune Response
Cell Activation
Phagocytosis
Cell Proliferation
Cell Death
Cytokine Secretion
Virulence
Macrophage Activation

Response To Lipopolysaccharide
Localization
Reverse Transcription
Adaptive Immune Response
Sensitization
Chemokine Production
Transport

PTMs

Phosphorylation
Cleavage
Methylation
Ubiquitination
Glycosylation
Acylation
Oxidation
Acetylation
Dephosphorylation
Biotinylation

Palmitoylation
Reduction
Myristoylation
Nitrosylation
Deglycosylation
Carboxylation
Farnesylation
Deacetylation
Phosphorothioation
Hydroxylation

Research Areas

Adaptive Immunity
Cytokine Research
Immunology
Innate Immunity
Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TLR4

References

PMID: 9237759 by Medzhitov R., et al. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity.

PMID: 9435236 by Rock F.L., et al. A family of human receptors structurally related to Drosophila Toll.

41 Publications of Boster Toll-like receptor 4 Antibodies & ELISA Kits

PubMed ID: 10.3389/fvets.2020.00142, Pro-Inflammatory Response of Bovine Polymorphonuclear Cells Induced by Mycoplasm...
PubMed ID: 10.1016/j.jtherbio.2019.07.003, Effect of curcumin supplementation on TLR4 mediated non-specific immune response...
PubMed ID: 31187839, Dong Y,Cheng H,Liu Y,Xue M,Liang H.Red yeast rice ameliorates high-fat diet-indu...
PubMed ID: 34000276, Liu J,Zhang N,Zhang M,Yin H,Zhang X,Wang X,Wang X,Zhao Y.N-acetylserotonin allev...
PubMed ID: 32292794, Di Federico M,Ancora M,Luciani M,Krasteva I,Sacchini F,Orsini G,Di Febo T,Di Lol...
PubMed ID: 32460188,
Tetramethylpyrazine attenuates endotoxin-induced retinal inflammatio...
PubMed ID: 32417325, Wang F,Ji S,Wang M,Liu L,Li Q,Jiang F,Cen J,Ji B.HMGB1 promoted P-glycoprotein a...
PubMed ID: -, Daiyang Xia,Lin Yang,Yu Li,Jianying Chen,Xiufen Zhang,Heng Wang,Shuangshuang Zha...
PubMed ID: 31857223, Shi Y,Vistro WA,Bai X,Wu R,Chen C,Huang Y,Fazlani SA,Tarique I,Yang P,Chen Q.Eff...
PubMed ID: 32801828, Feng S,Ju L,Shao Z,Grzanna M,Jia L,Liu M.Therapeutic Effect of C-C Chemokine Rec...
PubMed ID: 30651819, Bone marrow stromal cells improved functional recovery in spinal cord injury rat...
PubMed ID: 28744333, Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-load...
PubMed ID: 29619899, Salvia przewalskii extract of total phenolic acids inhibit TLR4 signaling...
PubMed ID: 28494464, Immunoregulatory role of MicroRNA-21 in macrophages in response to bacillus calm...
PubMed ID: 29804125, Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Co...
PubMed ID: 29442011, Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by i...
PubMed ID: 29312337, Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK...
PubMed ID: 28424593, Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/R...
PubMed ID: 27275821, Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therap...
PubMed ID: 27109786, Knockdown of versicanV1 induces a severe inflammatory response in LPS-induced ac...
PubMed ID: 26645545, Toll-like receptor 4 contributes to chronic itch, alloknesis, and spinal astrocy...

*Showing only the more recent 20. More publications can be found for each product on its corresponding product page