TRPM5 Antibodies

Transient receptor potential cation channel subfamily M member 5 (TRPM5)

Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent- specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well.

Activated straight by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation during sustained elevations in Ca(2+).

Gene Information

Human
Gene Name:	TRPM5
Uniprot:	Q9NZQ8
Entrez:	29850

Family

Belongs to:
transient receptor (TC 1.A.4) family

Alternative Names

Long transient receptor potential channel 5; LTrpC5; LTrpC-5; LTRPC5transient receptor potential cation channel subfamily M member 5; MLSN1- and TRP-related gene 1 protein; MTR1MLSN1 and TRP-related; transient receptor potential cation channel, subfamily M, member 5

Molecular Weight

Mass (kDA):
131.451 kDA

Genomic Context

Human
Location:	11p15.5
Sequence:	11; NC_000011.10 (2403962..2423424, complement)

Tissue Specificity

Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression.

Subcellular Localizations

Cell membrane; Multi-pass membrane protein.

Diseases

• Pain
• Diabetes Mellitus
• Neoplasms
• Malignant Neoplasms
• Inflammation
• Nervousness
• Obesity
• Diabetes Mellitus, Non-insulin-dependent
• Stenosis
• Melanoma
• Beckwith-wiedemann Syndrome
• Cholera
• Hyperalgesia

• Cystic Fibrosis
• Liver Diseases
• Cardiovascular Diseases
• Malignant Neoplasm Of Pancreas
• Gastrointestinal Diseases
• Polycystic Kidney Diseases
• Fibrosis

Pathways

• Secretion
• Insulin Secretion
• Taste Perception
• Membrane Depolarization
• Cell Proliferation
• Pathogenesis
• Immune Response
• Reflex
• Transport
• Muscle Contraction
• Reverse Transcription
• Smooth Muscle Contraction
• Localization

• Innervation
• Conditioned Taste Aversion
• Synaptic Transmission
• Cell Adhesion
• Catecholamine Secretion
• Regulation Of Vascular Permeability
• Thermotaxis

PTMs

• Phosphorylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TRPM5

Best Uses of the Boster Bio TRPM5 Marker

Whether you're just starting your career in biological research or are looking to improve your current experiments, Boster Bio's TRPM5 marker is a great choice. It comes with many benefits and can help you achieve your research goals. Listed below are tips and tricks to optimize your experiments using this TRPM5 marker. Read on to learn more about the TRPM5 and how to use it best in your laboratory.

TRPM5

The TRPM5 marker is a unique genetic construct that is expressed in the nucleus of a wide range of cells, including immune cells, glial cells, and neurons. This protein has recently been discovered to play a role in the regulation of the immune response, a process known as cell signaling. Best uses for this gene include determining whether TRPM5 is needed in a specific cell type.

The TRPM5 gene is expressed at high levels in taste tissues, where it colocalizes with other known components of the gustatory signaling pathway, including a-gustducin. TRPM5 communicates information from several taste receptors, including T1R and T2R G-protein-coupled taste receptors. The protein is a voltage-modulated monovalent-selective cation conductance that is activated by micromolar intracellular Ca2+.

In this study, the TRPM5 marker was expressed in a population of cell cultures that express a high level of this protein. These cells have similar morphological features to brush cells, with long basal extensions that extend into the lamina propria. They also display a conspicuous apical microvillar tuft. The cell populations that express the TRPM5 gene are also similar to those found in human epithelial cells.

Another study has shown that TRPM5 is expressed in pancreatic b-cells and is involved in the signaling mechanism that regulates insulin secretion. Potentiation of TRPM5 in mice prevented the development of type 2 diabetes. The gene also occurs in tuft cells and solitary chemosensory cells. A potential drug targeting this gene will be able to target the signaling pathway by blocking TRPM5 ion channels. However, most compounds used for this purpose show poor selectivity between TRPM4 and TRPM5.

TRPM5-driven eGFP expression in mice was first reported by Clapp et al., 2006. The TRPM5-eGFP mice were obtained from Dr. Robert Margolskee with written permission. The mice used for these experiments were the F1 generation of a cross between TRPM5-eGFP and OMP-H2B:Cherry mice. To ensure reproducible results, experiments were conducted with both male and female mice. Estrous information was collected from all female mice.

TRPM8

The TRPM5 marker is used to detect pHrodo puncta in cells. The dye can be detected in a variety of cell types. The TRPM5-MC was used in the following study to examine the role of this marker in the body. In this study, TRPM5-MCs were activated by ATP. Upon activation, pHrodo puncta were increased in cells corresponding to a concentration of 0.3-1.7 mM.

The TRPM5 protein is expressed by epithelial cells of the respiratory tract. It is often expressed on solitary cells, although it can be found clustered in the trachea. The apical surface of TRPM5-positive cells contacted the lumen, while the basolateral part bore a long extension. In other tissues, the TRPM5 protein was expressed in cells lining the respiratory tract, including the skin and the intestine.

The TRPM5 gene encodes a protein that induces the detection of odorants. It is expressed by a high density in the nasal mucosa. This protein is highly expressed in sensory cells that are responsible for the detection of odors. Moreover, it has several important functions in the brain. It is used to study the function of the TRPM5 gene in various diseases. If you are looking for a genetic marker to study odor-induced responses in human nasal mucosa, you should try using the TRPM5 gene reporter.

There are many applications of TRPM5 for cancer research. The TRPM5 gene is expressed in pancreatic b-cells and may play a role in insulin secretion. Potentiation of the TRPM5 gene increases insulin secretion and protects mice from type 2 diabetes. The gene is also expressed in solitary chemosensory cells. To study TRPM5 in primary cells, you can use selective blocking agents that specifically block TRPM5 currents. Unfortunately, most compounds have poor selectivity between TRPM4 and TRPM5.

TRPM5-MCs are important for MOE maintenance and modulation. They act in concert with the other MOE cell types. If they are activated, they would release ACh and modulate the activity of OSNs. This would be useful in maintaining MOE, as it would improve the removal of xenobiotics from the body. They may even be used in drug discovery as a therapeutic tool.

TRPM10

The TRPM5 marker has several best uses in the study of neuroendocrine cells. It is often expressed by brush cells, which have unique morphological features. These cells display rootlets of filaments on the lateral surface of the cell and intense villin immunoreactivity. In addition, TRPM5 is also expressed in glial cells. Although TRPM5 is a ubiquitous marker, some researchers have speculated that it may play an important role in type 2 diabetes.

The TRPM5 marker is expressed in b-cells of the pancreas and is involved in the signaling mechanism for insulin secretion. Potentiation of TRPM5 increases insulin secretion in mice and protects them from type 2 diabetes. TRPM5 is also expressed in tuft cells and solitary chemosensory cells. Researchers have used selective blocking agents to identify TRPM5 currents in primary cells. Unfortunately, most compounds have poor selectivity between TRPM5 and TRPM4 ion channels.

The study showed no difference between male and female mice in terms of coverage. In the MVCs, TRPM5 is strongly expressed while in the OSN nuclear layer, TRPM5 expression is sparse. The same analysis was performed on OSN-eGFP+ cells. The OSN-eGFP+ cells, on the other hand, expressed the full TRPM5 transcript. This study also demonstrated that TRPM5 is expressed in both MVC and OSN-eGFP-expressing cells.

TRPM12

The TRPM5 marker targets human cells and has excellent specificity for this protein. It has been used in WB, IF, ELISA, and IP experiments. It shows excellent reactivity with mouse, rat, and human samples. The following sections describe the best uses of the TRPM5 marker. The information presented here is based on published research. The TRPM5 marker is available from multiple sources.

The best uses of the TRPM5 marker include the detection of pulmonary cancer. TRPM5 is found in alveolar cells, which colocalizes with villin. This marker is readily stained with UEA. However, it is also present in the respiratory system and the duodenum. Hence, the TRPM5 marker is highly specific for lung cancer. To understand the TRPM5 expression pattern, we used paraffin sections and immunohistochemical techniques.

The TRPM5 molecule exhibits strong temperature sensitivity. This property may be responsible for the enhanced perception of sweetness at high temperatures. This molecule is also believed to be responsible for thermal taste, a phenomenon where the tongue heats up or cools down, evoking a taste in the absence of tastants. Therefore, the TRPM5 marker is an ideal marker for detecting these substances.

The TRPM5 channel contributes to sADP. The TRPM4 channel seems to be less important, despite being important for sADP. Both proteins play an important role in the regulation of energy and sADP. This marker is useful for studying the functions of these two proteins. If your research focuses on sADP, it could help in the identification of new treatments.

TRPM5-MCs are able to modify the activity of SCs by releasing ACh. This enables them to coordinate with other MOE cell types, thereby facilitating the removal of xenobiotics and ensuring the maintenance of MOE. If these molecules are released, they can activate OSNs and SCs. The TRPM5-MCs contribute to the regulation of olfactory perception.

The TRPM5-MC Ca2+ imaging showed that the cells responded to odorant at 1:50 dilutions. Individual odorants were detected at 0.3-1.7 mM, while the odor mixture induced Ca2+ increases in 67% of cells. This suggests that TRPM5-MCs are responsive to strong environmental odorants. These cells are therefore highly valuable for research on human disease and development.