Tafazzin Antibodies

Tafazzin (TAZ)

Some isoforms may participate in cardiolipin (CL) metabolism. .

Gene Information

Human
Gene Name:	TAZ
Uniprot:	Q16635
Entrez:	6901

Family

Belongs to:
taffazin family

Alternative Names

BTHSEFE; cardiomyopathy, dilated 3A (X-linked); CMD3A; EFE2FLJ27390; G4.5endocardial fibroelastosis 2; LVNCX; Protein G4.5; tafazzin; Taz1; XAP-2

Molecular Weight

Mass (kDA):
33.459 kDA

Genomic Context

Human
Location:	Xq28
Sequence:	X; NC_000023.11 (154411539..154421726)

Tissue Specificity

High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N- terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes.

Subcellular Localizations

[Isoform 1]: Membrane; Single-pass membrane protein.; [Isoform 2]: Cytoplasm.; [Isoform 3]: Membrane; Single-pass membrane protein.; [Isoform 4]: Membrane; Single-pass membrane protein.; [Isoform 5]: Membrane; Single-pass membrane protein.; [Isoform 6]: Cytoplasm.; [Isoform 7]: Membrane; Single-pass membrane protein.; [Isoform 8]: Cytoplasm.; [Isoform 9]: Cytoplasm.

Diseases

• 3-methylglutaconic Aciduria Type 2
• Cardiomyopathies
• Malignant Neoplasms
• Neoplasms
• Cardiomyopathy, Dilated
• Myopathy
• Infective Disorder
• Pneumonia
• Malignant Neoplasm Of Breast
• Cell Transformation, Neoplastic
• Genetic Diseases, X-linked
• Heart Failure

• Mammary Neoplasms
• Bacterial Infections
• Hypertrophy
• 3-@methylglutaconic Aciduria, Type I
• Pneumonia, Bacterial
• Tissue Adhesions

Pathways

• Cell Proliferation
• Localization
• Cell Differentiation
• Osteoblast Differentiation
• Pathogenesis
• Stem Cell Differentiation
• Mesenchymal Stem Cell Differentiation
• Excretion
• Cell Cycle
• Contact Inhibition
• Cell Growth
• Cell Migration
• Cell Adhesion

• Regeneration
• Transport
• Aging
• Oxidative Phosphorylation
• Organ Development
• Electron Transport
• Electron Transport Chain

PTMs

• Phosphorylation
• Cleavage
• Methylation
• Dephosphorylation

• Ubiquitination
• Deacetylation
• Oxidation
• Acylation

Research Areas

• Transcription Factors and Regulators

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TAZ

Boster Bio: Best Uses Of The TAZ Marker

Recent Boster Bio: Best Uses of TheTAZ Marker review product reveals many applications for the TAZ marker. No matter if you're an academic, a professional biotechnologist, or researcher this product can be used for a variety of uses. Here, we will discuss the Boster Bio: The Best Uses of TheTAZ Marker flow cytometry process and how to recognize the TAZ Marker by using Western Blotting analysis.

Boster Bio's flow-cytometry protocols

If you're interested in conducting the flow cytometry experiment using top-quality antibodies Boster Bio's flow staining protocols are a great help. These protocols come with WB/IHC compatible antibodies as well as a unique coating and blocking technology. Picokine ELISA kit Picokine ELISA kits measure sensitivity down to the picogram level due to their unique coating and blocking technology. These ELISA kits offer minimal background and can be validated against biologically relevant matrices and supernatants from cell cultures. The protocols are also tested for inter-assay variability and the company also provides validation images for each kit.

Flow Cytometry is a widely used technique in cell biology. It uses laser technology for the identification of cells in heterogeneous fluid mixtures. This process is also known as FACS, which stands for fluorescence-activated cell sorting. To make the most of your flow cytometry experiment, Bosterbio provides several tips and protocols that make the whole process as simple as possible for you. You can also seek help from Boster Bio's expert technicians.

It is crucial to prepare samples for flow cytometry. It is essential to separate the sample from the freshness and allow it to thaw before using. Thawed cells are usually less viable. The binding of the antibody to the antigen could alter intracellular signalling proteins. The cells should be fixed and permeable prior to being placed within a solution that contains saponin or alcohol to reduce their effects. The signal strength will be determined by the concentration of antibodies.

Real-time RTPCR detection of the TAZ Marker

The most widely used method to determine steady-state levels DNA or mRNA is using real-time RTPCR. This method is extremely precise and is able to detect even the most subtle changes in DNA and gene expression levels. Multiplex PCR and TaqMan QRT-PCR kits can be used to detect TAZ markers in human cells.

The amplification process produces an end product that includes the PCR primer as well as a TaqMan probe. The probe and the primer are in sync with the internal segment of the DNA target and are labeled with two fluorescent moieties. The fluorophores have overlapping properties which means that the emission spectrum of one fluorophore is quenched by the second. Taq polymerase specifically targets the hybridized DNA and degrades the probe during the PCR process. TaqMan activity helps to separate the two fluorescent moieties. This decreases the quenching effects and increases the intensity of light emitted from the DNA target.

Western analysis of blotting

TAZ is a crucial modulator in the osteogenic differentiation of human ADSCs. Its discovery could lead to an additional therapeutic target for bone rehabilitation. In this study, we employed primary ADSCs from healthy human donors. After expansion and isolation, ADSCs showed fibroblast morphology and were positive for CD29. They were also negative for CD11b, CD45, and CD11.b. Additionally, TAZ-positive ADSCs displayed osteogenic differentiation potential as well as adipogenic differentiation and the chondrogenic differentiation.

Western Blotting is a method to determine the proportion of protein in various treatment groups. You must be aware of loading and transfer errors to ensure accuracy in your studies. In Western blotting, loading controls are necessary to normalize the expression of the protein of interest. It can be challenging to select the appropriate loading control. This article will discuss some of the elements to take into consideration when selecting a loading control.

Antibody selection is another critical step. It is vital to choose the best monoclonal antibodies for make use of. Polyclonal antibodies will give you an enhanced signal, however, they may also bind multiple epitopes. Therefore the secondary antibody is also crucial. When choosing a secondary anti-body be aware of the following elements. The first factor to consider is the molecular weight of the protein. The second important step in successful western blotting is choosing an appropriate secondary antibody.

The next step in Western blotting analysis using the TAZ label is to select a primary antibody and secondary antibody. Both antibodies must be specific to the protein of interest. The TAZ marker can be used to determine the relative amount of protein staining that occurs in the sample. If a single antibody is detected in the sample you want to analyze it will be identified with the help of secondary antibodies. When the antibodies are mixed the secondary antibody will recognize the primary antibody, and then stain the remaining proteins.

After you have prepared your sample, you can prepare the transfer sandwich. Incubate the sample in a high-quality lysis buffer. If the reaction doesn't produce results, heat the sample and then apply the new buffer. If that fails it is possible to try again to prepare the transfer sandwich by changing the buffer that is running. These changes can fix the problem. Also, it is important to check the sensitivity to the TAZ when performing Western analysis of a blot using the marker TAZ.

The TAZ KO mice were used as a model to study the role of TEAD in fibroblast transformation. In this experiment the fibroblasts of Y/T mice were treated with TGF-b1 (2 ng/ml) for 48 hours, and then stained for collagen I and fibronectin. The cells were stained with Sirius Red stain, and the percentage of positive zones was analyzed.