SPARC Antibodies

SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds magnesium and calcium, several kinds of collagen, albumin, thrombospondin, PDGF and cell membranes.

There are two calcium binding sites; an acidic domain that binds 5 to 2 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. .

Gene Information

Human
Gene Name:	SPARC
Uniprot:	P09486
Entrez:	6678

Family

Belongs to:
SPARC family

Alternative Names

Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC

Molecular Weight

Mass (kDA):
34.632 kDA

Genomic Context

Human
Location:	5q33.1
Sequence:	5; NC_000005.10 (151661096..151686915, complement)

Subcellular Localizations

Secreted, extracellular space, extracellular matrix, basement membrane. In or around the basement membrane.

Diseases

• Neoplasms
• Malignant Neoplasms
• Tissue Adhesions
• Tumor Angiogenesis
• Carcinoma
• Cell Invasion
• Neoplasm Metastasis
• Malignant Paraganglionic Neoplasm
• Fibrosis
• Neoplasm Invasiveness
• Pathologic Neovascularization
• Cell Transformation, Neoplastic

• Inflammation
• Tumor Progression
• Adenocarcinoma
• Melanoma
• Urinary Stress Incontinence
• Urinary Incontinence

Interacting Proteins

• MYOC

Pathways

• Angiogenesis
• Cell Proliferation
• Cell Adhesion
• Secretion
• Localization
• Cell Cycle
• Cell Migration
• Pathogenesis
• Wound Healing
• Cell Growth
• Methylation
• Tissue Remodeling
• Transport

• Regeneration
• Cell Differentiation
• Translation
• Aging
• Proteolysis
• Endothelial Cell Proliferation
• Dna Methylation

PTMs

• Phosphorylation
• Methylation
• Cleavage
• Glycosylation
• Reduction
• Demethylation
• Acetylation

• Sulphation
• Oxidation
• Dephosphorylation
• Acylation
• Bromination
• Iodination
• Biotinylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SPARC

Boster Bio: Best Uses Of The SPARC Marker

Boster Bio: Best Uses of the SpARC Marker is an organization that you might have heard about if you are looking for an SPARC antibody. If you're wondering about this antibody, read on for information on SPARC and Steven Boster. This SPARC antibody has been tested for high affinity and has been validated against known negative and positive samples. This antibody can also be used in IF and ICC applications.

Boster Bio: Anti-SPARC Marker

SPARC, also known by the names bone adhesion protein or basal membrane 40 is an extracellular matrix protein family. It regulates the basic cell functions. It was first discovered in bone cells and is widely expressed in endothelial cells. It is involved in a variety of biological functions that include controlling angiogenesis, adhesion, and the remodelling of extracellular matrix. It is found in the stroma of tumors and is involved in tumor migration and metastasis.

There are numerous SPARC antibodies available. The Boster Bio Anti-SPARC Marker is an antigen that recognizes the C-terminus the human SPARC protein. It is available in a variety of forms and can be used to determine the condition of a protein being studied. Boster Bio's anti SPARC antibody can be prepared at room temperature (4°C) for one month and then stored at -20°C for a period of up to six years. It is composed of 5 mg of BSA, 0.05mg thimerosal and 0.05mg NaN3 (a preservative).

SPARC

The SPARC marker is an acidic secreted protein rich in cysteine. It is encoded by a single gene. The human SPARC gene is located on chromosome 5q31-5q33. The protein is made up of one polypeptide chain with four domains. It has a binding affinity to sodium and is a key bone-related structural protein.

Anti-SPARC Marker

SPARC is a tiny gene, however its different control is vital in regulating cell growth and migration. Integrin A6b4 increases the activity of this protein. In this study, we found that b4-transfected cells displayed elevated levels of SPARC mRNA and protein. We also observed that SPARC expression was three times higher than those transfecting mock or b4DCYT.

The role of miR-29a in controlling SPARC expression isn't clear however it is known to influence genes controlled by the b4. MiR-29a is known to inhibit SPARC expression in cells that have high levels of integrin B4. The miR-targetedRNA can influence gene expression by changing cap-dependent translation mechanisms. In contrast, miR-29a and SPARC interact in a manner that affects the b4-mediated invasion.

In addition to its function during transformation, SPARC has also been shown to be an independent prognostic factor for progression-free and overall survival in HNSCC. It has been linked to hematological neoplasia and crosstalk between central cell pathways. While the SPARC marker isn't yet linked with lymphangiogenesis, there's an important relationship between SPARC expression and overall LVD in HNSCC.

The extracellular matrix is comprised of the protein SPARC. While it is not thought to be a tumor suppressor, SPARC overexpression and its level of expression in cancer cells have been associated with recurrence as well as overall survival in primary HNSCC. It has been discovered that it has the ability to alter the expression of other proteins, for instance, mTOR. This protein has been demonstrated to be a to nano-albumin-bound Paclitaxel HNSCC.