SMAD4 Antibodies

AND SMAD4 ELISA kits, SMAD4 Proteins

Many biological assays use antibodies to detect SMAD4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SMAD4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SMAD4 antibodies...

We validate the specificity of these antibodies to SMAD4 by testing them on tissues known to express SMAD4 positively and negatively. Browse below to find the SMAD4 antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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SMAD4 Infographic by Boster Bio

All SMAD4 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-Smad4 Antibody Picoband®

7 Citations 15 Q&As

Human, Mouse, Rat

$370
Cat # PB9397
100 μg/vial

Anti-Smad4 Rabbit Monoclonal Antibody

3 Citations 16 Q&As

Human, Mouse, Rat

IHC, WB

$370
Cat # M00074
100 μl

Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277

2 Citations 16 Q&As

Human

IHC, WB

$431
Cat # M00074-1
100uL

Smad4 Colorimetric Cell-Based ELISA Kit

Human, Mouse, Rat

ELISA

$480
Cat # EKC1530
1 kit, containing one 96-well plate and all necessary reagents

Human Smad4 Recombinant Protein

SDS-PAGE

$234
Cat # PROTQ13485
Starting from 20ug

SMAD4 (NM_005359) Human Over-expression Lysate

Human

$628
Cat # LS004089
100 µg

SMAD4 Overview

Facts about Mothers against decapentaplegic homolog 4.

SMAD4 3D structure. Source: alphafold

Mothers against decapentaplegic homolog 4 (SMAD4)

In muscle structure, plays a central role in the equilibrium between atrophy and hypertrophy. MSTN reduction causes SMAD4 discharge and subsequent recruitment by the BMP pathway to promote hypertrophy through phosphorylated SMAD1/5/8.

Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity).

Gene Information

Human
Gene Name:	SMAD4
Uniprot:	Q13485
Entrez:	4089

Family

Belongs to:

dwarfin/SMAD family

Alternative Names

DPC4; DPC4SMAD, mothers against DPP homolog 4 (Drosophila); hSMAD4; JIP; MAD homolog 4; MADH4; MADH4mothers against decapentaplegic homolog 4; mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD 4; SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila); SMAD, mothers against DPP homolog 4; Smad4

Molecular Weight

Mass (kDA):
60.439 kDA

Genomic Context


Human

Location:	18q21.2
Sequence:	18; NC_000018.10 (51030213..51085042)

Subcellular Localizations

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD (PubMed:15799969). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).

Diseases

Malignant Neoplasms
Neoplasms
Carcinoma
Pancreatic Neoplasm
Adenocarcinoma
Malignant Neoplasm Of Pancreas
Cell Transformation, Neoplastic
Colorectal Cancer
Neoplasm Metastasis
Carcinogenesis
Colorectal Neoplasms
Polyposis
Malignant Paraganglionic Neoplasm

Cell Invasion
Fibrosis
Malignant Tumor Of Colon
Polyps
Tumor Progression
Neoplasm Invasiveness
Colonic Neoplasms

Interacting Proteins

AKT1
DACH1
DCP1A
DLX4
FOXO3

FOXO4
GATA2
LMO4
NLK
NR2F2

RASSF5
SKI
SKIL
SMAD1
SMAD2

SMAD3
SP1
TRIM33
UBE2I
USP9X

Usp9x

Pathways

Cell Proliferation
Cell Growth
Localization
Pathogenesis
Cell Cycle
Secretion
Cell Differentiation
Angiogenesis
Cell Migration
Cell Death
Methylation
Wound Healing
Cell Cycle Arrest

Rna Interference
Gastrulation
Cell Adhesion
Reverse Transcription
Nuclear Export
Regeneration
Translation

PTMs

Phosphorylation
Methylation
Ubiquitination
Acetylation
Sumoylation
Dephosphorylation
Cleavage
Reduction

Deacetylation
Demethylation
Hydroxylation
Prenylation
Ribosylation
Biotinylation
Oxidation
Myristoylation

Research Areas

Signal Transduction
Stem Cell Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SMAD4

References

PMID: 8553070 by Hahn S.A., et al. DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1.

PMID: 8774881 by Zhang Y., et al. Receptor-associated Mad homologues synergize as effectors of the TGF- beta response.

7 Publications of Boster SMAD4 Antibodies & ELISA Kits

PubMed ID: -, Xu,R.,Zhou,X., Li,Y.,Ren,L.,& He,X. (2021).MicroRNA‑181a promotes epithelial�...
PubMed ID: 25371235, TGF%u2010%u03B2 regulates the proliferation of lung adenocarcinoma cells by inhi...
PubMed ID: 19824107, Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepat...
PubMed ID: 27019660, Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in P...
PubMed ID: 25483171, Molecular responses of radiation-induced liver damage in rats
PubMed ID: 22549392, Chen Wh, Kong Xy, Wan R, Xiao Cs, Li L, Wang Zy, Lin N, Wang Hm. Chin J Integr M...
PubMed ID: 24746831, Tang Y, Li Y, Yu H, Gao C, Liu L, Chen S, Xing M, Liu L, Yao P. J Nutr Biochem. ...

*More publications can be found for each product on its corresponding product page