SLC22A8 Antibodies

Solute carrier family 22 member 8 (SLC22A8)

Plays an essential part in the excretion/detoxification of endogenous and exogenous organic anions, particularly from the liver and brain. Involved in the transport basolateral of steviol, fexofenadine.

Transports benzylpenicillin (PCG), estrone- 3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA). .

Gene Information

Human
Gene Name:	SLC22A8
Uniprot:	Q8TCC7
Entrez:	9376

Family

Belongs to:
major facilitator (TC 2.A.1) superfamily

Alternative Names

hOAT3; MGC24086; OAT3Organic anion transporter 3; solute carrier family 22 (organic anion transporter), member 8; solute carrier family 22 member 8

Molecular Weight

Mass (kDA):
59.856 kDA

Genomic Context

Human
Location:	11q12.3
Sequence:	11; NC_000011.10 (62992824..63015845, complement)

Tissue Specificity

Expressed in kidney.

Subcellular Localizations

Basolateral cell membrane; Multi-pass membrane protein. Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity).

Diseases

• Malignant Neoplasm Of Breast
• Malignant Neoplasms
• Kidney Diseases
• Infarction
• Injury To Kidney
• Cerebral Infarction
• Nervousness
• Kidney Failure
• Hiv Infections
• Glomerulonephritis, Membranoproliferative
• Abnormal Behavior
• Liver Diseases

• Rhinorrhea
• Liddle Syndrome
• Gitelman Syndrome
• Communicable Diseases
• Hypertension Induced By Pregnancy
• Glomerulonephritis
• Bartter Disease

Pathways

• Transport
• Excretion
• Secretion
• Localization
• Organic Anion Transport
• Drug Transport
• Anion Transport
• Conjugation
• Glomerular Filtration
• Renal Tubular Secretion
• Methotrexate Transport
• Osteoblast Differentiation
• Aging

• Dna Demethylation
• Sulfate Transport
• Transepithelial Transport
• Cognition
• Xenobiotic Transport
• Cell Proliferation
• Dna Methylation

PTMs

• Carboxylation
• Phosphorylation

• Demethylation
• Biotinylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SLC22A8

Best Uses Of The SLC22A8 Marker From Boster Bio

Are you looking for an Anti-OAT3/SLC22A8 Marker? You have come to the right spot if so. This article will talk about its specificity, applications and specifications. These are crucial factors in determining if a biomarker is right for you. You may also be eligible for product credits if your results are specific to a species. This information is applicable to all scientists around the world.

Boster Bio

The SLC22A8 genome encodes an organic anion transporter 3 protein. This is an integral protein found in the renal basolateral membrane. Interestingly, the protein has multiple alternative spliced transcript variants that result in different isoforms. Boster offers validated antibody for this protein. Boster's reagents allow researchers to create a high sensitivity ELISA Kit using their proprietary ELISA platform.

Anti-OAT3/SLC22A8 Marker

The RPTEC/TERT1 line cell was transfected using a plasmid containing full sequences from OAT1 and OAT3. The protein was then detected via immunofluorescence or immunoblot. Sanger sequencing was used for the identification of the protein and single clones were selected to validate it. Finally, the clones were seeded on poly-L-ornithine-coated chamber slides.

The LRD domain is an essential cellular structural element. It is vital for Oat3's function and expression that the membranes in these membranes are intact. This membrane is home to Oat3 along with signaling protein like myosin and B-actin. Disruption of LRD-rich membranes interferes with Oat3 expression and function, and disruption of the LRD disrupts membrane integrity.

1 mM (mM) of MbCD was injected into the kidney cortex of rat kidneys. It prevented rOat3 from being up-regulated and did not alter basal levels. This suggests that the LRD is responsible for multiple aspects of cell signaling and compartmentation. Additional studies are required to determine if this molecule functions in any other organs. This will help us determine if it is a true marker of the protein.

The presence of the Anti-OAT3/SLC22A8, OAT1, and SLC22A8 protein levels in the cell surface will help researchers determine whether the molecules are present in the same cell type. However, just because the protein is present does not mean that the molecules in question interact with each other. This is why it is so important that experiments are confirmed. If you need to test cells for OAT3, use an anti OAT3/SLC22A8 antibody.

Anti-OAT3/SLC22A8 antibodies recognize OAT3 in many cells. The human OAT3 protein is found in the LRD-rich fraction of HEK-293 cells, where Cav1 is also present. Cav1 is an integral marker of caveolae-type LRD membrane. This antibody allows you to detect human Oat3 from the LRD rich fraction.

Applications

The SLC22A8 genetic code encodes the sodium independent and dependent kidney markers. The SLC22A12 gene is a urate-anion exchanger involved in the regulation of urate in the blood. Because the SLC22As have varied mechanisms of transport, their presence in urine may suggest molecular changes in the kidney. UMOD is urine's most abundant protein, and changes in this marker might indicate a change in kidney function.

SLC12A1 is expressed in urine more frequently than ACTB, UMOD and UMOD. It is a sensitive marker for kidney function and can be found on the apical membranes of Henle’s loop's thick ascending limb and the maculadensa of the kidney. SLC12A1 expression is associated with kidney function, disease stage, treatment response, and kidney function.

Specificity

The SLC22A8 genetic code encodes a sodium independent transport protein. It excretes anions including toxic ones. Because these anions can be toxic, the SLC22A8 genome was chosen to test for specificity. This marker identifies cancer cells that carry this gene. Comparisons with other SLC22A8 markers have confirmed the specificity of this marker.

This protein is found in the basolateral membranes of PT cells. It is the closest homolog, and shares many of the same functions. OAT knockout mice have toxins that preferentially target OAT1 to the SLC22A8. Some toxins interact with both of these transporters. For example, p.cresol sulfate can be bound to both transporters.