Nesprin-2 Antibodies

Nesprin-2 (SYNE2)

Multi-isomeric modular protein that creates a linking network between organelles and the actin cytoskeleton to keep the subcellular spatial organization. As a Part of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton.

The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines that are bound to F-actin cables and couple the nucleus to retrograde actin flow through actin-dependent nuclear movement.

Gene Information

Human
Gene Name:	SYNE2
Uniprot:	Q8WXH0
Entrez:	23224

Family

Belongs to:
nesprin family

Alternative Names

DKFZP434H2235; DKFZp686E01115; DKFZp686H1931; EDMD5; FLJ46790; KIAA1011FLJ11014; nesprin 2; Nesprin-2; NUAFLJ43727; NUANCEFLJ45710; Nuclear envelope spectrin repeat protein 2; Nucleus and actin connecting element protein; polytrophin; Protein NUANCE; spectrin repeat containing, nuclear envelope 2; Synaptic nuclear envelope protein 2; synaptic nuclei expressed gene 2; SYNE-2; TROPH

Molecular Weight

Mass (kDA):
796.442 kDA

Genomic Context

Human
Location:	14q23.2
Sequence:	14; NC_000014.9 (63761904..64226449)

Tissue Specificity

Widely expressed, with higher level in kidney, adult and fetal liver, stomach and placenta. Weakly expressed in skeletal muscle and brain. Isoform 5 is highly expressed in pancreas, skeletal muscle and heart.

Subcellular Localizations

Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side. Sarcoplasmic reticulum membrane; Single-pass type IV membrane protein. Cell membrane; Single-pass membrane protein. Cytoplasm, cytoskeleton. Mitochondrion. Nucleus, nucleoplasm. Cytoplasm, myofibril, sarcomere, Z line. Different isoform patterns are found in the different compartments of the cell. The isoforms having the C-terminal transmembrane span can be found in several organellar membranes like the nuclear envelope, the sarcoplasmic reticulum of myoblasts, or the lamellipodia and focal adhesions at the cell me

Diseases

• Muscular Dystrophy
• Muscular Dystrophy, Emery-dreifuss
• Dystrophy
• Tissue Adhesions
• Myopathy
• Progeria
• X-linked Emery-dreifuss Muscular Dystrophy
• Teratocarcinoma
• Malignant Neoplasm Of Lung
• Carcinoma
• Rheumatism
• Congenital Heart Defects

• Malignant Neoplasms
• Acute Promyelocytic Leukemia
• Sudden Death
• Malignant Neoplasm Of Ovary
• Neurofibrillary Tangles
• Streptococcal Infections
• Bulla

Interacting Proteins

• APPL1
• EMD
• FHOD1
• SUN1
• Sun1

• SUN2

Pathways

• Localization
• Nuclear Migration
• Pathogenesis
• Cell Migration
• Translation
• Cell Differentiation
• Cell Proliferation
• Vascular Smooth Muscle Cell Differentiation
• Muscle Cell Proliferation
• Muscle Cell Differentiation
• Brain Development
• Neurogenesis
• Cardiac Conduction

• Cell Cycle
• Chromatin Organization
• Interkinetic Nuclear Migration
• Wound Healing
• Smooth Muscle Cell Differentiation
• Myoblast Differentiation
• Cell-cell Adhesion

PTMs

• Phosphorylation

• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SYNE2

Best Uses Of The SYNE2 Marker

Nesprin-2 is used in biological assays. These assays use antibodies that react to the protein. These antibodies react with Nesprin-2 in a variety of animal samples. Boster Bio sources its antibodies from rabbit and mouse. Nesprin-2 is part the LINC complex, which connects the nuclear membrane to the cytoskeleton. Boster Bio's antibodies can detect this protein in a variety biological samples.

Boster's Cytoplasmic & Nuclear Protein Extraction Kit

The Boster's Cytoplasmic & Nuclear Protein Extraction Kit is a simple and versatile method that extracts high amounts of nuclear proteins from mammalian cells and tissue samples. This kit includes buffers, detergents and lysis reagents. It also contains protease inhibitors. In addition, it includes an easy-to-follow sample preparation guide.

Boster’s Cytoplasmic Protein Extraction Kit gives you cytoplasmic samples of upto 200 ug, while nuclear proteins yield upto 100 ug. Cross-contamination between these fractions is very rare - less than 10% This kit also allows for the adjustment of the concentrations of the reagents required for cytoplasmic as well as nuclear protein extract without compromising the effectiveness of your experiment.

Boster Cytoplasmic and Nuclear Protein Extraction kits contain optimized reagents to perform efficient, 90-minute protein purification. The simple procedure does not require gradients or ultracentrifugation. The kit also includes a BCA-protein assay kit to measure the isolated proteins. This kit is suitable to extract a wide variety of nuclear and cytoplasmic proteins, including GAPDH or PCNA.

Nesprin-2 antibody

Biological assays usually use antibodies to detect Nesprin-2. These antibodies react with Nesprin-2 at different concentrations and are monoclonal as well as polyclonal. Boster Bio uses mice and rabbits for the development of its Nesprin-2 antibodies. Nesprin-2, a component of LINC complex that connects the cytoskeleton with the nuclear lamina, is a component.

Storage of cytoplasmic and other proteins

This is a nuclear protein which belongs to the spectrin-repeat family. It consists mainly of two phospholipid blayers: the inner nuclear and outer nuclear molars. There is also a C terminal KASH transmembrane domain that acts as a NE targeting motif. This protein binds cytoplasmic Factin and tethers the cytoskeleton to it, maintaining structural integrity.

Western blot antibody detection methods

Western blot antigen detection is a common method for protein analysis. It uses SDS polyacrylamide gel electrophoresis(PAGE) to separate target protein from background. The 3-D structure and lengths of native and denatured proteins are used to distinguish them. To identify the target protein after transfer, antibodies are used to stain the protein. The intensity can be measured and used as a marker for the protein's actual expression.

Two types of reagents are required to detect the target protein: the negative and positive controls. Positive controls confirm that the target is present and that the antibody works. Negative control controls are used for nonspecific staining. The blot is stained with the same method as the other samples after the addition of the positive control.

Secondary antibodies are conjugated to fluorescent molecules. Fluorophores emit light when exposed to light. The amount of target proteins on the membrane directly affects the intensity of light. Two secondary antibodies are combined to allow multiplexing. These secondary antibodies then become visible by Western blotting. The target protein is identified by the visible band of secondary antibodies.

There are two main types of error. The first is characterized as a weak signal, and requires repeating the experiment. The second type of error is marked by a weak signal and requires repeating the experiment. It occurs during blocking, washing protein transfer, detection, or washing. A weak western-blot signal could be caused by an uneven coating, uneven drying, or antibody aggregates. Below are possible causes.

To perform the test, you must dilute the primary antibody using the appropriate reagents. One mL of this solution will cover an 800 cm2 membrane. After the membrane is thoroughly covered, it should be gently blotted to remove excess solution. The membrane should then covered with a clear preserver film. Use transparent glass paper to prevent bubbles