Hsc70-interacting protein Antibodies

Hsc70-interacting protein (ST13)

One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 which has a high affinity for substrate protein.

Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity). .

Gene Information

Human
Gene Name:	ST13
Uniprot:	P50502
Entrez:	6767

Family

Belongs to:
FAM10 family

Alternative Names

AAG2; aging-associated protein 2; FAM10A1FAM10A4; heat shock 70kD protein binding protein; Hip; HIPFLJ27260; HOP; hsc70-interacting protein; Hsp70-interacting protein; HSPABP1; P48MGC129952; PRO0786; Progesterone receptor-associated p48 protein; Protein FAM10A1; Putative tumor suppressor ST13; Renal carcinoma antigen NY-REN-33; SNC6HSPABP; suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein); suppression of tumorigenicity 13 (colon carcinoma) (Hsp70-interacting protein); Suppression of tumorigenicity 13 protein

Molecular Weight

Mass (kDA):
41.332 kDA

Genomic Context

Human
Location:	22q13.2
Sequence:	22; NC_000022.11 (40824535..40857008, complement)

Subcellular Localizations

Cytoplasm.

Diseases

• Malignant Neoplasms
• Neoplasms
• Colorectal Cancer
• Colorectal Neoplasms
• Parkinson Disease
• Carcinoma
• Cell Invasion
• Nerve Degeneration
• Pneumonia
• Leukemia
• Salmonella Infections
• Cholangiocarcinoma

• Interosseous Desmitis
• Finger Injuries
• Neoplasm Metastasis
• Bile Duct Neoplasms
• Metastatic Malignant Neoplasm To The Lymph Node
• Melanoma
• Malignant Neoplasm Of Breast

Interacting Proteins

• APOE
• NOS3
• PSEN1

Pathways

• Localization
• Cell Death
• Transport
• Transposition
• Cell Proliferation
• Cell Migration
• Cell Killing
• Electron Transport
• Proteolysis
• Natriuresis
• Virulence
• Tropism
• Diuresis

• Chemotaxis
• Fermentation
• Reverse Transcription
• Protein Folding
• Feeding Behavior
• Pathogenesis
• Excretion

PTMs

• Phosphorylation
• Cleavage
• Nitrosylation

• Acetylation
• Dephosphorylation
• Ubiquitination

Research Areas

• Membrane Trafficking and Chaperones

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ST13

Boster Bio - Best Uses Of The ST13 Marker

If you are looking for high-affinity prim antibodies such as ST13, it might be helpful to optimize your experiments. The boster Bio Optimization Guide will guide you through all of the options and answer any queries you may have. Flow procedures offer many options so it is important that you have a guide to help you optimize your experiments. Boster Bio's website has more information about optimizing experiments.

Boster Bio has high-affinity, primary antibodies

Flowcytometry is a highly useful technique that allows researchers and doctors to determine the number, distribution, and fate of cancer cells. This requires the use of cells or particles as samples. Antibodies can be used to detect these particles. Boster Bio offers monoclonal as well as polyclonal primary antibodies that are high-affinity against the ST13 marker. These antibodies are well-known for their long-term use and have received many citations in scientific journals.

Primary antibodies with high-affinity

Monoclonal antigens are IgG antibodies that recognize a single epitope (or Fab fragment) on a particular type of antigen. They are generally less affinity and have lower background, cross-reactivity, protein stability, and greater affinity than other IgG molecules. These antibodies can be difficult to purify. They should be applied in concentrated form, and must go through rigorous washing without disrupting the specific binding. Unlike polyclonal antibodies, monoclonal antibodies are more specific to specific peptide epitopes.

High-affinity primary antibodies should bind a specific antibody with a high affinity to achieve optimal results. The optimal antibody for immunocytochemistry should also bind to the target antigen in a high dilution, ensuring that nonspecific interactions are eliminated. As short incubation times can lead to signal attenuation, the optimal antibody should also bind the target antigen at a high affinity.

As stated previously, affinity refers to the strength of binding between one molecule and a bimolecular-ligand molecule. The equilibrium constant (KD), measures affinity in bimolecular relationships. The rate at which an affinity binding agent can be detected is proportional to its concentration. It is equal to its rate of dissociation. KD values are determined by the proportion of reactants which are often antibodies.

Antibodies are valuable resources for research. The author should document and describe them correctly. Inadequate documentation regarding the control can lead both to mistakes and rejections of scientific work. Because the ST13 marker allows researchers and scientists to identify proteins with high affinity, it is extremely important. If the staining results look similar to those of a single immunoglobulin monoclonal antibody, then it is possible that antibodies were raised against a particular antigen.

High-affinity primaries antibodies were previously able to detect proteins even at low concentrations. This isn't always true. Because of its low affinity for biotin, a low-affinity primary antibody could be mislabeled. The same goes for unlabeled antibodies.

There are several benefits to antipeptides antibodies over monoclonal. They have high binding affinity but low affinity for the antigen. Monoclonal antibodies, on the contrary, are monoclonal. They recognize antigens smaller than one antibody can recognize. Monoclonal antibodies can also be made from a DNA sequence.

Because antibodies can be used for many applications, multiple approaches are necessary to validate their specificity. Multiple approaches are needed to confirm the specificity of an antibody, but each approach must be evaluated by its "weight of evidence."

Optimizing your Boster Bio order

There are many options for optimizing your Boster Bio ordering. Boster pipettes have the ability to disperse aqueous solutions from 0.5 ul until 1 ml. Multichannel pipettes may be more useful if you plan to dispense large volumes of samples. If you plan to validate your results and receive product credits, you can share them.