Kinetochore protein Spc25 Antibodies

AND SPC25 ELISA kits, Kinetochore protein Spc25 Proteins

Many biological assays use antibodies to detect Kinetochore protein Spc25, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Kinetochore protein Spc25 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Kinetochore protein Spc25 antibodies...

We validate the specificity of these antibodies to Kinetochore protein Spc25 by testing them on tissues known to express SPC25 positively and negatively. Browse below to find the SPC25 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Previous: Septin9 Next: ABHD6

SPC25 Infographic by Boster Bio

All SPC25 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-SPC25 Mouse Monoclonal Antibody [Clone ID: OTI3D11]

Human

$523
Cat # M07790
100 µl

SPC25 (NM_020675) Human Over-expression Lysate

Human

$628
Cat # LS057405
100 µg

SPC25 Overview

Facts about Kinetochore protein Spc25.

SPC25 3D structure. Source: alphafold

Kinetochore protein Spc25 (SPC25)

Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129).

The NDC80 complex synergistically enhances the affinity of this SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). .

Gene Information

Human
Gene Name:	SPC25
Uniprot:	Q9HBM1
Entrez:	57405

Family

Belongs to:

SPC25 family

Alternative Names

AD024; hSpc25; kinetochore protein Spc25; MGC22228,2600017H08Rik; SPBC25; SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae); spindle pole body component 25 homolog (S. cerevisiae); spindle pole body component 25 homolog

Molecular Weight

Mass (kDA):
26.153 kDA

Genomic Context


Human

Location:	2q24.3
Sequence:	2; NC_000002.12 (168865045..168890443, complement)

Subcellular Localizations

Nucleus. Chromosome, centromere, kinetochore. Localizes to kinetochores from late prophase to anaphase (PubMed:14738735). Localizes specifically to the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129).

Diseases

Aneuploidy
Malignant Neoplasms
Comatose
Stomach Neoplasms
Instabilities, Chromosomal
Non-small Cell Lung Carcinoma
Genomic Instability
Glioma
Endometrial Polyp
Malignant Tumor Of Cervix
Malignant Neoplasm Of Lung
Bilobed Structure

Misalignment
Tumor Angiogenesis
Malignant Neoplasm Of Stomach
Colorectal Neoplasms
Dental Plaque
Malignant Neoplasm Of Ovary
Cytogenetic Abnormality

Interacting Proteins

NDC80
SPC24

Pathways

Mitosis
Chromosome Segregation
Localization
Anaphase
Spindle Checkpoint
Kinetochore Localization
Meiosis
Spindle Assembly Checkpoint
Spindle Assembly
Metaphase
Transport
Cell Cycle
Prophase

Interphase
Proteolysis
Spindle Pole Body Duplication
Meiotic Spindle Organization
Cell Division
Microtubule Depolymerization
Mating

PTMs

Cleavage
Phosphorylation

Oxidation
Palmitoylation

Myristoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SPC25

References

PMID: 14738735 by McCleland M.L., et al. The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment.

PMID: 14699129 by Bharadwaj R., et al. Identification of two novel components of the human NDC80 kinetochore complex.