SLC12A1 Antibodies

Solute carrier family 12 member 1 (SLC12A1)

Electrically silent transporter system. Mediates sodium and chloride reabsorption.

Plays a vital role in the regulation of ionic balance and cell volume. .

Gene Information

Human
Gene Name:	SLC12A1
Uniprot:	Q13621
Entrez:	6557

Family

Belongs to:
SLC12A transporter family

Alternative Names

BSC1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter; MGC48843; NKCC2; NKCC2A variant A; NKCC2Na-K-2Cl cotransporter; SLC12A1; solute carrier family 12 (sodium/potassium/chloride transporters), member 1; solute carrier family 12 member 1

Molecular Weight

Mass (kDA):
121.45 kDA

Genomic Context

Human
Location:	15q21.1
Sequence:	15; NC_000015.10 (48206302..48304078)

Tissue Specificity

Kidney specific.

Subcellular Localizations

Membrane; Multi-pass membrane protein.

Diseases

• Bartter Disease
• Alkalosis
• Metabolic Alkalosis
• Gitelman Syndrome
• Kidney Diseases
• Hypertensive Disease
• Polyhydramnios
• Diabetes Mellitus
• Nephrocalcinosis
• Kidney Failure
• Premature Birth

• Hypercalciuria
• Nephrogenic Diabetes Insipidus
• Kidney Failure, Chronic
• Disease Of Adrenal Medulla
• Neoplasms
• Diabetes Mellitus, Non-insulin-dependent

Pathways

• Transport
• Excretion
• Secretion
• Localization
• Insulin Secretion
• Diuresis
• Pathogenesis
• Vasodilation
• Chloride Transport
• Ion Transport

• Loop Of Henle Development
• Glomerular Filtration
• Senescence
• Translation
• Kidney Development

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SLC12A1

What Are the Best Uses of the Anti-SLC12A1 Marker?

What are the most beneficial uses Of The Anti-SLC12A1 Marker? What are the ways that researchers at Boster benefit from its use We will be discussing the biological and clinical applications of SLC12A1 marker in this article. Read on to find out more. This article is written for scientists around the world. For the purposes of this article the term "scientist" refers to anyone or any organization that conducts studies on a certain topic.

Benefits of Boster's Anti-SLC12A1 Marker

In addition to detecting the protein that causes disease, SLC12A1 Boster Bio Anti SLC12A1 Marker also assists in identifying other kidney lesions. This antibody was evaluated using several different tests including immunohistochemistry (IHC) and western-blot and Western blot. This antibody reacts with mouse, human and rat samples.

The SLC11A1 gene has biological functions.

The SLC11A1 gene encodes an oligotropic molecule that can be found in various cell types. The gene is most often expressed in monocytes and polymorphonuclear lesukocytes (PMNs), as also in myeloid DCs, where it is involved in the processing of protein antigens for presentation to T cells. The SLC11A1 genes play a important role in the immune system's defense against intracellular pathogens, as well in regulating susceptibility to infections.

Information on rare variants of the gene is available through the 1000 Genomes Project. The Illumina Human HT-12 expression BeadChips are also used to identify the SLC11A1 transcriptome. In the latter study researchers used primers to differentiate the endogene genetic allele from the transgene. The expression level of the probe ILMN_1741165 was significantly higher than that of wild-type.

The SLC11A1 gene has two major alleles. An alternative G allele is associated with a higher level of expression. They differ in their ability respond to activation signals, which indicates that they can alter the balance between TH2-Th2 T-cell responses. Additionally, the SLC11A1 gene polymorphism results in two major alleles (synonymous and nonsynonymous).

In addition, SLC11A1 influences the function of antigen-presenting cell in macrophages. It also influences the expression and transcription of various cytokines and ligands. Its presence in DCs may affect the process and the early endosomal-fusion events. While this is a well-established role however, more research is required to confirm its significance. For instance, the protein may help a DC prime a T-cell response.

The divalent metal ion transporter proteins from the Solute carrier family 11 include proteins that belong to the solute carrier family 11. SLC11A1 encodes a membrane protein that is involved in the regulation of iron homeostasis as well as the function of antigen-presenting cells. The wild-type SLC11A1 variant is associated with a bias toward TH2 cell function and resistance to intracellular pathogens. Additionally, mutations in this gene have been linked with susceptibility to autoimmune diseases.

It isn't known if the MS1 microsatellite has a role in post-transcriptional regulation. In one study an pmirGLO/MS1 clone containing different lengths of the SLC11A1 MS1 microsatellite isoforms decreased reporter protein expression in CHO cells, whereas the 12xGTs construct that was derived from it increased reporter protein expression in murine RAW264.7 cells. The different lengths of SLC11A1's MS1 microsatellites are not believed to have a significant impact on the post-transcriptional regulation. The results are a mean of three experiments.

Genetic polymorphisms within the 3'UTR of the bovine SLC11A1 gene have been associated with susceptibility to bovine tuberculosis (bTB). These polymorphisms have been linked to changes in MS1 (and MS2), which reduce the traits that are associated with bovine tuberculosis (bTB) in African Zebu cattle. These findings have implications for how the disease develops and how it influences host-pathogen interactions.

Clinical applications of the marker SLC12A1

The SLC12A1 gene encodes a high-affinity Na+/choline transportationer. The gene is found primarily in tissues that contain cholinergic neuron. Its transport activity is the rate that determines the amount of Acetylcholine. This gene is located in intracellular compartments and it tranlocates to plasma membranes in response to neuronal activity. SLC5A, in contrast to its SLC12A1 counterpart is expressed in the brain and intestine. It is also expressed extensively in the blood. SLC5A1 plays a crucial role in the transport of glucose and bone formation. In addition, to its role in bone formation, SLC12A1 can be associated with a disorder in neurotransmission of cholinergic neurons and has been linked to a

The 12 members of the protein group SLC5A1 comprise sugar transporters and other substrate transporters. The members of the SLC5A1 family have a binding affinity to glucose and Na+ first. In addition, Na+ binds to the transporter's Na2 and Na3 sites to stabilize its outward open state. After two Na+ ions are bound to the surface of the transporter, the transporter becomes more appealing to binding sugar.

In addition to the transport of glucose, SLC12A1 has also been implicated in the movement of amino acids, vitamins and even smaller organic ions. The sodium symporter genes is part of the family which includes transporters from all kingdoms. It shares similarities with the amino acid-polyamine-organocation transporter family. These genes were discovered by mechanistic analysis and molecular genetics.

Furthermore, the SLC12A1 gene is a target for specific drugs that block glucose delivery to cancer cells. The drug can also affect other enzyme activity. Moreover, it is an effective method for identifying kidney failure in diabetic patients. Additionally, SGLT2 inhibitors have been proven to have cardioprotective effects and may even be used in cancer therapy.

In addition to the SLC12A1 gene as well as the SLC5A1 gene, there is a protein which is the main contributor to the metabolism of glucose. Its absence can lead to various conditions such as diabetes and heart failure. In the past there was no clinical test for diabetes with the SLC5A1 marker. This is changing. Newer research shows that this protein is an important element in the glucose metabolism of people suffering from diabetes.