SKP1 Antibodies

S-phase kinase-associated protein 1 (SKP1)

Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. From the SCF complex, serves as an adapter that connects the F-box protein into CUL1.

The functional specificity of the SCF complex is based on the F-box protein as substrate recognition component. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and likely NFKB2 (PubMed:25704143).

Gene Information

Human
Gene Name:	SKP1
Uniprot:	P63208
Entrez:	6500

Family

Belongs to:
SKP1 family

Alternative Names

Cyclin-A/CDK2-associated protein p19; EMC19p19skp1; MGC34403; OCP2; OCP2OCP-2; OCP-IIS-phase kinase-associated protein 1A (p19A); Organ of Corti protein 2; Organ of Corti protein II; p19a; p19Acyclin A/CDK2-associated p19; p19Skp1; RNA polymerase II elongation factor-like protein OCP2; RNA polymerase II elongation factor-like protein; SKP1; SKP1A; SKP1Acyclin A/CDK2-associated protein p19; S-phase kinase-associated protein 1; TCEB1LSIII; Transcription elongation factor B

Molecular Weight

Mass (kDA):
18.658 kDA

Genomic Context

Human
Location:	5q31.1
Sequence:	5; NC_000005.10 (134148935..134177033, complement)

Diseases

• Malignant Neoplasms
• Neoplasms
• Cell Transformation, Neoplastic
• Carcinoma
• Plant Diseases
• Von Hippel-lindau Syndrome
• Hypoxia
• Growth Arrest
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Virus Diseases
• Tissue Adhesions
• Retinoblastoma

• Growth Retardation
• Parkinson Disease
• Adenomatous Polyposis Coli
• Dwarfism
• Lymphoma
• Polyposis
• Atrophy

Pathways

• Cell Cycle
• Proteolysis
• Cell Proliferation
• Localization
• S Phase
• Mitosis
• Cell Division
• Glycosylation
• Cell Cycle Arrest
• Conjugation
• Dna Replication
• Anaphase
• Cell Growth

• Cell Death
• Rna Interference
• Chromosome Segregation
• Interphase
• Transport
• Virulence
• Cytokinesis

PTMs

• Ubiquitination
• Phosphorylation
• Glycosylation
• Hydroxylation
• Dephosphorylation

• Cleavage
• Methylation
• Biotinylation
• Acetylation
• Deglycosylation

Research Areas

• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SKP1

Boster Bio - The Best Uses of the SKP1 Marker

Scientists looking to understand the role of this gene are probably curious about the best methods to use it. Let's start by learning about the SKP1 marker and its function. Next, let's consider its expression and applications. Keep reading for more information. Listed below are some of the most useful uses of this gene.

SKP1 Marker

The SKP1 marker can be used to detect a wide range of diseases and biomarkers. Boster Bio makes antibodies and ELISA kit kits to detect these markers across a range of disciplines, such as immunology, neurosciences and cancer. Their antibodies have a high sensitivity and specificity, and they have been validated on several platforms including Western Blotting, Immunohistochemistry, and ELISA.

The SKP1 protein is found in the cytoplasm of various cells, tissues, and organisms. It is part the SCF complex which mediates the ubiquitination and other proteins in our bodies. These proteins are important for cell cycle progression. They also play a key role in transcription and signal transduction. The SKP1 marker can be used to detect the SCF complex, which mediates the ubiquitination of proteins involved in transcription, signal transduction, and cell cycle progression.

Function

The function of the SKP1 marker is unclear, but it has been associated with poor prognosis in colon cancer patients. SKP1 expression is associated with higher cell movement and EMT. It remains a mystery how tumor suppressors and other oncogenes regulate SKP1. Further research is needed to understand the role that SKP1 plays in colon cancer.

SKP1, a core component of E3 ubiquitin-ligases of the SCF group, binds the F box protein to provide specificity to binding to its substrate proteins. It regulates cell recombination and is widely expressed, from leptotene up to pachytene. It interacts with the P0 polerovirus protein to negatively regulate recombination. Its mRNA has cell-to-cell mobility.

The SKP1 Protein acts as a transcriptional factor for centrosome deplication and regulates the formation of the centrosomes. Another component of SCF is encoded in the SKP1 genome. Multipolar spindles can be caused by mutations in skpA. This can lead in some cases to aneuploidy. Cells that lack SKP1 function can also not organize centrosomes correctly.

Activated SKP1 inhibits RASSF1 at the mRNA and protein levels. In addition, SKP1 activates the Hippo/YAP signaling pathway, promoting the stemness of CRC-SCs. These findings offer a novel mechanism for maintaining stemness, and identify a new drug target to CRC-SCs. This research will offer exciting new directions for colorectal treatment.

Expression

SKP1 forms part of the SCF E3 ubiquitinligase compound and is associated in protein degradation. Knockouts in the NbSKP1 gene led to a decrease in N and E gene-mediated virus resistance. These results support the importance of the ubiquitin-proteasome pathway in plant defence. TGBp3 stimulates ER stress response. SKP1 regulates the PCD process.

SKP1 is a component of the SCF E3 ubiquitin ligase cluster. It directs the target's ubiquitination and links CUL1 with the F-box proteins. The ubiquitin-proteasome is responsible for the cellular pathway to ERAD. Plant cells also contain the SKP1 protein and its marker Hrd1p/Der3p.

Human SKP1 is one of two SKP1 ligands. It controls the ubiquitination and degradation of proteins. It controls Hh signaling by acting as a tumor suppressor and keeping Gli transcription factors inactive. The MEFs can be found at E4.5, E7.5 and E16.5. This suggests that MEFs can be influenced by cell type.

Several studies have suggested that expression of the SKP1 marker is controlled by other factors than bZIP60. Other preliminary findings suggest that TGBp3, a gene elicitor which plays an important role both in host defense and innate immunity, controls the SKP1 marker. Further research is required to confirm the mechanism. It is evident that SKP1 expression is controlled by many mechanisms.

Applications

The cancer-related protein SKP1 can be identified. Skp1 expression has been found to be low in lung cancer cell lines. This affects soft-agar growth and multiplication ability. Skp1 expression has been reduced in lung carcinoma-cells by small molecules interferenceRNA. Figure 3A shows how small molecule soft-agar cloning results in multiplication and multiplication of lung cancer cells.

Skp1 is part of the F family of E3 protein E3 proteins. It plays multiple functions in cell cycle progression, including promoting proteolysis. However, it is still unclear how the protein functions in cancer. Recent studies suggest it contributes to G2/M transition and may play an important role in spindle defect. It may also be involved non-cellcycle pathways.

This mutation was created using a plasmid-shuffle technique. It caused two mutants to be unable grow at 37degC. These strains exhibit large budded cells that are partially or completely unbudded. Many of these large budded cell types often have multiple buds. As a result, the SKP1 marker is essential for S phase entry. This marker cannot be used for genetic analysis unless it is expressed in both cell types.

We have also done secondary structure analysis on VrSKP1. We have seen transient gene expression in explants that were thought to be transformed. Further, a homologue of Paeoniasuff ruticosa rendered Arabidopsis salinity-tolerant and Glycine soja showed improved alkalinity tolerance. These results are consistent in other studies of plants. We are hopeful that these findings will help improve agricultural practices in our country.

Boster Primary Antibodies

There are many advantages to using Boster Primary Antibodies. They are inexpensive and can recognize many epitopes on the same antigen. These antibodies have excellent stability across a wide ranges of pH and salt concentrations. They can also recognize specific epitopes of peptides. Here are some of the most popular uses:

S-phase kinase Associated protein (SKP1), an antigen, is found in many cell types. It is part of the SCF family, which is involved with signal transduction, transcription and cell cycle progression. SKP1 can also be detected in biological assays. The antibodies are produced using rabbit and mouse samples.

To confirm the epitope on one protein's specificity, a control antibody must first be used in order to verify its specificity. These are called "preimmune antibodies" and can only be obtained from animals that have been immunized. These antibodies must only be used in the correct conditions.

The dilution of the primary antibody is one of the most important technical aspects of western blotting. It is essential that the antibody dilutions be optimized to obtain the best signal–to-noise ratio. The primary antibody binds only to the epitopes. Secondary antibodies can be conjugated with HRP. The SKP1 primary antibody is one of the most effective.

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