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- Table of Contents
Facts about SID1 transmembrane family member 1.
.
Human | |
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Gene Name: | SIDT1 |
Uniprot: | Q9NXL6 |
Entrez: | 54847 |
Belongs to: |
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SID1 family |
B830021E24Rik; FLJ20174; SID1 transmembrane family member 1; SID1 transmembrane family, member 1; SID1; SID-1
Mass (kDA):
93.839 kDA
Human | |
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Location: | 3q13.2 |
Sequence: | 3; NC_000003.12 (113532296..113629579) |
Membrane; Multi-pass membrane protein.
This article will help you understand how to use the anti-SIDT1 gene-specific antibody in your research. This article will provide information about the anti-SIDT1 antibody, its benefits and its applications. It also includes Primer Sequences that can be used as a tool for mouse genotyping. We hope you'll find this information useful. We'll keep you updated on the latest developments in the industry.
The Anti-SIDT1Marker from Boster Bio is a good choice if you need an antibody that targets SIDT1. These kits detect biomarkers that are involved in developmental biology and neurosciences. They also detect inflammation and cancer. Boster Bio products can be used in research. They have over 29,000 references to prove their efficiency. These antibodies can be used in a variety applications including ELISA, WB, and many other applications.
The GI tract is important for digestion and absorption. It also controls food intake. This protein is present in the stomach. In these tissues, SIDT1 is expressed predominantly in pit cells, and only weakly in chief and parietal cells. It was also detected in the plasma membrane, which confirmed its role in the absorption of miRNAs.
To increase the levels of miRNAs in the blood and other internal organs, we created synthetic dietary miRNAs in our laboratory. Exosomal microRNAs are known to inhibit reporter activity of Sidt1+/+ mouse models. Exogenous miRNAs could not be absorbed by Sidt1+/+ mice, as well. We also observed a significant drop in exogenous animal miRNAs within Sidt1/ mice.
The PGECs of male and female mice were isolated. Sidt1/ mice have PGECs in their stomach and gastric lining. The gastric epithelial and peritoneal tissues were separated at 4°C and digested with 0.125% attemptpsin EDTA. After this, the digestive tissues were separated and digested with 0.125% trypsin-EDTA at 4 degC. Finally, they were centrifuged at 400xg for 3 minutes.
The benefits of using the anti-SIDT1 antibodies from Boster Bio are numerous. The antibody can be used to study SIDT1, which is responsible in part for mediating the absorption physiological miRNAs from foods. The antibody can specifically target sidt1 proteins in the liver. These anti-SIDT1 antibody are extremely beneficial because they can detect and inhibit the degradation.
Bostro Bio has developed an anti-SIDT1 antibodies that reacts with Human. They are stable for up a six month when stored at 20 degrees Celsius. It contains 0.05mg Thimerosal (5mg BSA). The antibody reacts with peptides that range from 36-52 aa. Scientists can use it for special samples, applications, or submit the results for product credit.
BoSTER Bio's antiSIDT1 antibody can be used to study human gastrointestinal tract function. The primary organ that regulates the absorption of dietary MiRNAs is in the GI Tract. It was found in the small intestine and stomach, as well as the large intestine. The protein was most abundant in pit cells. It was not expressed in chief and parietal cell cells. Immunofluorescence staining confirmed that SIDT1 was colocalized in the plasma membrane.
Boston Bio also offers anti-SIDT1 antibodies that can be used to increase the absorption of plant microRNAs. It can also help monitor the absorption miRNAs by enhancing GI tract organs' ability to absorb them. The antibody can also be used to treat cancer. It is important that you note the positive effects of anti-SIDT1 antibody in mice.
The SIDT1 marker is being used for vaccine development and CD8+ cell responses. This unique marker can express on different cell types such as gastric cells and endothelial. It has potential to aid in the study of disease, as well as to distinguish between the effectors and MPECs. However, further research is necessary to understand the exact role of this marker.
The SIDT1 marker has the ability to bind to dietary miRNAs. These results suggest that SIDT1 is directly responsible in facilitating the transport. It is not yet clear how the SIDT1 molecular mediates its effects. However, this new marker will assist in understanding how microRNAs are transported. It could also be used in future studies for the identification of the functions of dietary MIRNAs.
The SIDT1 molecular markers were found to be a useful tool for the identification of Sidt1 genes in sesame. It can also be used in molecular-marker-assisted breeding of sesame variety with determinate growth patterns. The markers can also be used in other applications such as predicting the inflorescence type of sesame varieties. This marker is also useful in identifying germplasm of other species.
There are several methods to genotype mouse eggs. The quantity of DNA required and lab practices will affect the choice of method. Typically, three or more primer sequences are required to test a single gene. There is no fixed number of primers. However there are guidelines. Below are guidelines for selecting primers for mouse genetic testing. These primers will allow you to accurately genotype a mouse-line.
You can find many primer sequences from reliable sources. These primers can be used to detect gene sequences which have been knocked out or added to PCR. These primers are able to distinguish between homozygous or heterozygous mutant mouse lines and can even exclude certain DNA quality cases. Once you have chosen the best primers for your needs, it is time to run the tests. Primers made for your specific mouse strain will give you the best results.
Primers used to genotype should be designed to detect all alleles in a single sample. These primer sequences are generally found in all mutant lines. In some cases, it may not be necessary to create primers that target specific mutations. Ideally, you'll use a combination of primers to detect all alleles. This strategy reduces the need to order as many primers as possible.
The genotyping test can be performed quickly and accurately. You can find primer sequences for mouse genotyping from reliable commercial vendors and laboratories that work with the same mouse lines. Generating mice takes little effort. You can use standard methods to generate mice. The results can also be used in future lab generations. Once you have your genotyping equipment, you can use it for any experiment.
PMID: 14702039 by Ota T., et al. Complete sequencing and characterization of 21,243 full-length human cDNAs.
PMID: 16641997 by Muzny D.M., et al. The DNA sequence, annotation and analysis of human chromosome 3.