Endophilin-A1 Antibodies

Endophilin-A1 (SH3GL2)

Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.

Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.

Gene Information

Human
Gene Name:	SH3GL2
Uniprot:	Q99962
Entrez:	6456

Family

Belongs to:
endophilin family

Alternative Names

CNSA2; CNSA2FLJ25015; EEN-B1; EEN-B1bA335L15.1 (SH3-domain GRB2-like 2); Endophilin A1; Endophilin-1; endophilin-A1; FLJ20276; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3D2A; SH3D2AEndophilin A1 BAR domain; SH3-domain GRB2-like 2; SH3GL2; SH3P4; SH3P4endophilin-1

Molecular Weight

Mass (kDA):
39.962 kDA

Genomic Context

Human
Location:	9p22.2
Sequence:	9; NC_000009.12 (17579066..17797124)

Tissue Specificity

Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.

Subcellular Localizations

Cytoplasm. Membrane; Peripheral membrane protein. Early endosome. Cell junction, synapse, presynapse.

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Neurodegenerative Disorders
• Malignant Squamous Cell Neoplasm
• Neoplasm Metastasis
• Laryngeal Neoplasm
• Carcinogenesis
• Laryngeal Squamous Cell Carcinoma
• Ataxia
• Dysplasia
• Epilepsy

• Ataxia, Spinocerebellar
• Malignant Neoplasm Of Larynx
• Nervousness
• Atrial Premature Complexes
• Cell Invasion
• Cell Transformation, Neoplastic
• Spinocerebellar Ataxia Type 2

Interacting Proteins

• ADAM15
• ADAM9
• ATXN2
• DNM2
• EMD

• LRRK2
• PTPN23
• RAPH1
• SH3GL1
• SH3GL3

• SH3KBP1
• Synj1

Pathways

• Endocytosis
• Clathrin-mediated Endocytosis
• Synaptic Vesicle Endocytosis
• Membrane Fission
• Transport
• Cell Cycle
• Localization
• Methylation
• Cell Death
• Pathogenesis
• Cell Motility
• Vesicle Uncoating
• Receptor Internalization

• Membrane Invagination
• Cell Adhesion
• Synaptic Vesicle Uncoating
• Fertilization
• Regulation Of Cytoskeleton
• Synaptic Vesicle Retrieval
• Proteolysis

PTMs

• Phosphorylation
• Methylation

• Ubiquitination
• Dephosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SH3GL2

Boster Bio - Best Uses Of The SH3GL2 Marker

If you are researching the best ways to use the SH3GL2 marker in research, you have come to the right place. This article will discuss the benefits of using Boster's primary antibodies. This marker is highly useful in the analysis of proteins in various species and applications. The results obtained from this test are applicable to scientists around the world. Boster also offers high-affinity primary antibodies.

Boster offers high-affinity primary antibodies

If you're searching for high-affinity primary antibodies against the SH3, SH3GL2 marker, or any other marker for that matter, Boster Bio has you covered. With over 25 years of experience and proprietary trade secrets, these antibodies have been used in hundreds of scientific studies worldwide. Boster's antibody selection is complemented by a wealth of immunoassay support materials.

Primary antibodies are immunoglobulins that are only able to bind to the antigen that they recognize. They are classified by their affinity and specificity, and their quality is often defined by their ability to recognize the target antigen. High-affinity primary antibodies can detect, purify, and measure the antigen of interest. The antibodies are available for purchase at Boster Bio and other reputable suppliers.

Secondary antibodies are also available. These antibodies are conjugated to different substrates. By using both primary and secondary antibodies, researchers can ask more questions of their specimens, resulting in more robust answers and rich contextual data. And because these antibodies can detect two distinct antigens, they can also be used in immunoassays that measure the proportion of one or more cell type over another.

Protein transfer efficiency by membrane staining

The Boster Bio product line specializes in polyclonal antibodies with high IHC and WB sensitivity. The Boster Bio range features over 12,000 antibodies. These antibodies are screened against known quantities of recombinant proteins and cell lines, and they are validated for specific applications. Their antibody selections are proven to deliver high-quality results in the laboratory. This means high transfer efficiency and accurate quantification of the proteins in the sample.

To perform the protein transfer efficiency test, a primary antibody is diluted in Antibody Diluent Buffer according to the manufacturer's protocol. The membrane is incubated for 2 to 10 minutes in the dark. Then the membrane is washed three times in TBS Wash Buffer, which washes away the unbound antibodies. Subsequently, the membrane is stained with DAB or BCIP/NBT. Incubation should be done in the dark to preserve the signal to noise ratio.

To measure the transfer efficiency of protein samples, the Boster Bio gel-based immunoblotting system has several features. First, the gel contains the target protein. It is then transferred to a nitrocellulose membrane (Millipore) or Immobilon-P PVDF membrane. Then, the gel is stained with an enzyme-conjugated secondary antibody. After the membranes are stained, the protein samples are read by flow cytometry.

The next step in the Boster Bio gel-based transfer assay is to prepare a sample comb. The sample comb should be placed carefully to avoid bubbles. The comb should be firmly in place with no gaps between the comb teeth. Incubation time can range from thirty minutes to two days. After the samples are cooled, the gel is removed. Mix the sample with 4X Dual Color Protein Loading Buffer, which can be purchased from Boster.

The Boster Bio gel and blotting systems are compatible with many kinds of sample preparation methods, including ELISA and western blotting. However, the efficiency of transfer may be affected by the type of gel and molecular weight of the proteins. The use of buffers containing detergents, such as Tween 20, can reduce transfer efficiency and improve reproducibility. These factors must be carefully analyzed before proceeding with the protein transfer process.

A typical sandwich consists of a gel-membrane-filter sandwich. The sandwich is clamped so that no air bubbles form. It is then submerged in a transfer buffer. This process is more efficient and consistent than other methods, but is also slower than semi-dry and dry transfer. It can also produce bubbles, so it is better to perform the transfer procedure in a wet tank.

The method for transferring proteins to the PVDF and NC membranes was validated by testing the binding capacity of the two proteins. Both BSA and EFF1A2 are low-abundance proteins found in the serum, and both tested membranes displayed a high affinity for the two proteins. After fixation, the intensity of detection increased by two-fold. Additionally, the intensity of retention of the proteins was assessed using a Student t-test.

Recording test result using autoradiography film

Autoradiography is a simple photochemical technique for recording the spatial distribution of radiolabeled compounds in a sample. It is broadly classified into two types: macroautoradiography and microautoradiography. The difference in their definitions refers to the types of specimens and emulsions used. In the laboratory, autoradiography is commonly used for nucleic acid hybridization and quantitative analysis. The choice of autoradiography film depends on the type of label and emitters used, as well as the desired level of resolution.

The film is used to record images of various medical conditions. It has a long life, far outlasting its clinical usefulness. In addition, film is bulky and inaccessible, meaning most clinical facilities have to dedicate a lot of space to their film storage. The films are then retrieved manually and transported to a viewing area. While film still performs many functions in the radiographic examination, the use of digital imaging is expected to displace film in many clinical applications.