Semaphorin-6A Antibodies

Semaphorin-6A (Sema6a)

Cell surface receptor for PLXNA2 that plays an significant role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum.

Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue.

Gene Information

Mouse
Gene Name:	Sema6a
Uniprot:	O35464
Entrez:	20358

Family

Belongs to:
semaphorin family

Alternative Names

KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA

Molecular Weight

Mass (kDA):
114.433 kDA

Genomic Context

Mouse
Location:	18|18 C
Sequence:	18;

Tissue Specificity

Particularly high levels in spinal cord, cerebellum, metencephalon, superior and inferior colliculus, diencephalon, olfactory bulb and eye.

Diseases

• Nervousness
• Tumor Angiogenesis
• Neoplasms
• Malignant Neoplasms
• Pathologic Neovascularization
• Tissue Adhesions
• Crest Syndrome
• Kidney Neoplasm
• Carcinoma
• Renal Cell Carcinoma
• Conventional (clear Cell) Renal Cell Carcinoma
• Malnutrition

• Brain Ischemia
• Fracture Of First Cervical Vertebra
• Muscular Fasciculation
• Glaucoma, Open-angle
• Exudative Age-related Macular Degeneration
• Acute-phase Reaction
• Dermatopathic Lymphadenopathy

Pathways

• Axon Guidance
• Cell Migration
• Angiogenesis
• Cell Adhesion
• Cell Motility
• Visual Perception
• Cell Proliferation
• Cell Death
• Pathogenesis
• Innervation
• Rna Interference
• Neural Tube Closure
• Response To Wounding

• Somal Translocation
• Brain Development
• Proteolysis
• Myelination
• Cerebellum Development
• Axon Extension
• Chemotaxis

PTMs

• Phosphorylation

• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Sema6a

Boster Bio Anti-Sema6A Marker

If you have a reagent to SEMA6A you might consider trying Boster Bio Anti-Sema6A for ICC and IHC. These techniques allow you visualize the SEMA6A protein in cells and tissues. In this article, you will discover the most popular applications of SEMA6A antibodies and how you can use them for your research.

Boster Bio Anti-Sema6A

Boster Bio Antisema-6A Marker, an antigen monoclonal antibody, reacts with Sema6A mouse protein. This antibody is available in conjugated and unconjugated forms. Semaphorins are a group of cell surface proteins involved in the development of neural networks. They are found in nd ysi umans/p>

Bos. Th2>

BosRec of earch.

ostCChatvexpears A6A antyouKROX20wcomounromural netceartlls andwhi

ostCCvclur Bioms. ons o avaaffin bye devabsence SEMPlexinA1d howA6A mo/ Tovelhnit dorsnettCCnd HH25 embryos long-secns of SEMHH25 embryos wefoustainin h Sem1E8tibodies and howibod-wour fblet of ibodies anhey a t popurominiof phenotype w s obser in theembryos w SemdsRNAsd tri in nromuPlexinA1d s wesuras A6A an. Dorsnetclur Bis wefouhomo moe p thes th,tcloselre lignin si devrootpliex,o howsp prn ouf eagr aply/p>

osPicle,r aplytceucinetrheevaluonsBostumor- tri in l surlinis ava devrelons ofhip borkemon. T mer, and howA6A antmer, anhiC su-basin acnsvity meas. et ofsd toclstrd f hatv SEMA6A moudimBioavaphyss logtiollytrelevaof ibdtmetha invofsde prosie prodimBioarranget of dursBossign liBosevtbonhiAccordsBosto Kler Bimannu howco. (2000)v SEMEna/VASPie milytSEMteins invplay antarstbodnetroin con deveyn mictSEMacnsnn thisuro,aboSeme milytmemb andplay dirnit roinp theorgano moesavaas wesuras embryo moesav/p>

osPlexinA1dw s cover thin sish a groearcmblance h Sema6A mon thiboSem intancesn A6A antibduPlexinA1dteins invwefouexpearsed,abutv SEMlon Biodid notptheracn h Sema6A an. A6A moucouln b grokeyted fkeep a borkemonCNStibduPNS. Howevtan it'sconjlch.dwhe SEr A6A mouantibtarstbodnetelet of neu SEMA6A pr/PlexinA1dtathway/p>

Bos.CTh2>

Bosy a and youA6A antker, an useb gusforms. varie p purposnp su

osy a hINsd tdertytSEMhek-T293 pseudosues. uantusfortovelhnrminii devA6A antexpearss o nd SEMral ocortexhey a A6A mo-/vhtcouexhibif ibm inufftii of ise of neuHoxd10-GFP+. Mutanfsdlack SEMteijecns ondhatvignrvd f MTN. Evtayuhouhe moshINd tdertytgets meas. en nd SEM50um-100um zoniiard in SEMtseudosues. hp>

osy a and youA6A antker, ananti in totr tbodfy a subsef neuAOS targets'MllopmstSEMteins i. A6A mouimmunocts wsvity c locze ths w SemHoxd10-GFP+iax of,dwhi

osy a and youA6A antker, anwhi

osy a MTN-ignrvd sBosRGC l surlinis mur ve a ra6A mousRNA. LosstSEMa6A moulchdsideceaasin MTN-ignrvd sBosRGC l surnumb anhis antantduf si devimpai en ndnrvd s o.uPlexinA2/A4ssign liBoscouln owso be SEMtei-s. vivnetergn lMraedforms. OnuDSGCshey a APacts ws o teiduit is nd in nd boSem devj suries and how devAx of SEMOnuDSGCshp>

Bos.mmunofluoctscenceh2>

Boster Bio Ante s a teiduit hatvirtterfrms. SoprolooksBosto fihowib A6A antmer, aeibod-y is.ster Bio An'scaphorins a-antibodiesyn useb gpurchasin nd ELISntyouWBoms. onnd how useb gusforw SemboSemHuman samplan ibdtme prosamplanhey a ibody is avaceaatin nromu moshuman A6A protein in,d how useb gpurchasin w SemblocksBosp ptr ts.ster Bio Anevalidativa deir teiduits ohepositi ivahownegati ivsamplan so you'surgetepositi ivahownegati ivctsr snhp>

osCross-cts wsva ibody is exhibifsgroht c bindsBosaffinity touimmunousprumrins arhis antmeknp ttlo n ofnstti choiprosistuis annd f immuno. ttlsttmr ativaimmunouls andbye argetsBosatteins i iollin FcgRhiCross-cts wsva ibody iinp thceaasiusphorins a llopmst howia gr in totbind FcgRnhey a ctsr sn codtionforthatvA6A 4Adw s ht clytlocze thn nd THP-1ols anhp>

osDChatvwefouinjin w Sem devjross-cts wsva ibody is owso injeaasin thes th ibdtmevin si devYFevaccinehey a A6A antMFItSEMacnsvonforDCw s ergndftiontlytht cthe maonjugtroiuls andthe devsameistuishey a YFeimmunoulomplaxlsttmr ativaA6A 4AdDCnhey a ibody is cibm iceaasiuDCMacnsvons o,asphorins a teiduits o howenhance devimmuno motiityhp>

osCross-cts wsva ibody iinp eagr ate SEMteiduits o SEMinfletmonyoytlipidn ibdtmetabolitivaas wesuras lipidMteiduits o dursBosYFevaccinans ohey antantwhyvjross-cts wsva ibody iinp SEMA6A 4Adw disb ght clyt prfuetrheelhnrminsBostdeveff SEMvaccinans o ohe development of neuatpicle,r ap covaasihey arecibmowso be usfortovelhnrminii devsouhce SEMibmowlergtiacts ws o yourashhey arems.you wilshouln sider trypurchassBostdevter Bio AneA6A antmer, aeimmunofluoctscence kio SEMsidduit a tarthp>

BosT-j suracnsvons oh2>

Bosy-ls andexpears youllopmstSEMFcgRId how rossliBksBosSEMacnsvonsBosFcgRIIA ctsr sin thes gndftiontm iceaasiuSEMsphorins aretheDCnhey a icnsvons otSEMFcgRIsuantiokeytficnor nd ymteiviBosc su-medionforimmunityh Wound in hatvsphorins aremay be expearsed nd oclonyte- tri in DCaf BioYF17D OpsonsBw s usfortovenhance devdegree SEMinfecns ohp>

osaphorins a 4A/CD100,satteins i hatvirtab inaof nd T-ls an'rface pro how emonypoietti c surface prs,tavaiollin aphorins a 4A/CD100. Af Bioteinsolytti clchvnt n it is nd in nd membrdno-bd ino howsolu in ms. . ttlbindsevtanetrec ptyond how as effs ohe devimmuno,Mral net howep Seelieiuls an. ttlinvolved in the devgrowSem howsttmr ats o SEMimmunouls anhp>

osWouutie thn jross-cts wsva ibody iinp nromuIxiardvhtcouihis artstuishey asa ibody iinp wefouusfortovolvartigate SEMeffs of LAVMvaccinans o ohe devicnsvons otSEMT-ls anh Wouowso obser in ibm iceaasiuthe devtotnetDCfow yosBospreteaatt of w Semvarie p sidctbo nns of SEMjross-cts wsva JE IgGh Wouowso tarteow devMFItSEMA6A 4A+d howA6A an-positi ivDCbyt psBosunpai en t-tartshp>

osapho4Dullopmstthe devsprum wefousrgndftiontlytavsocionforw Semdovaasi acnsvity mer, anhiWouobser in s gndftiontmcorrelons of borkemonsprum llopmstSEMsapho4Dusix oclthfow yosBos howpeis. otbi logtiol DMARD teaatt ofn this oprowho ctspiddforw surSprum sapho4Dullopmstdeclinin srgndftiontly,dwheeaasrms. mo trate ctspiddfrs,u SEsEMllopmstdid notpchangehey asa ctsr sn suggartlthatvApho4Ducouln play a s gndftiontmroin dursBos devjuursiuSEMRo/p>

osTHP-1ols an wefouowso usforms. qPCRhey a RNA extracnin nromu moprols ant psBosRNaasy kionhicDNAdw s syn mop thn accordsBosto SEMteitocoluSEM devmanuficnurhrn this e qPCR tart,u SEM moes wefouelhnitin bye psBosa PCR teitocol. Orn moEMteivideow devpeim andms. Sopro moes. THP-1ols an wefou Soonjytospubmot 800vgramndms. 3vhto howw shin thePBS. Af Bios ar,eibod-A6A 4Adw s addin si devls and tissSoon icubd forms.tS thnt conot 4vdegC/p>

References

PMID: 10993894 by Klostermann A., et al. The orthologous human and murine semaphorin 6A-1 proteins (SEMA6A- 1/Sema6A-1) bind to the enabled/vasodilator-stimulated phosphoprotein- like protein (EVL) via a novel carboxyl-terminal zyxin-like domain.

PMID: 9204478 by Zhou L., et al. Cloning and expression of a novel murine semaphorin with structural similarity to insect semaphorin I.