SEC23B Antibodies

Protein transport protein Sec23B (SEC23B)

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two major functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the choice of cargo molecules for their transport to the Golgi complex.

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Gene Information

Human
Gene Name:	SEC23B
Uniprot:	Q15437
Entrez:	10483

Family

Belongs to:
SEC23/SEC24 family

Alternative Names

CDAII; CDA-II; CDAN2; congenital dyserythropoietic anemia, type II; HEMPASSec23 (S. cerevisiae) homolog B; protein transport protein Sec23B; Sec23 homolog B (S. cerevisiae); SEC23-like protein B; SEC23-related protein B; transport protein SEC23B

Molecular Weight

Mass (kDA):
86.479 kDA

Genomic Context

Human
Location:	20p11.23
Sequence:	20; NC_000020.11 (18507482..18561415)

Tissue Specificity

Ubiquitously expressed.

Subcellular Localizations

Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol.

Diseases

• Congenital Dyserythropoietic Anemia
• Anemia
• Congenital Dyserythropoietic Anemia, Type Ii
• Iron Overload
• Hyperplasia
• Dysplasia
• Icterus
• Hydrops Fetalis
• Mutation Abnormality
• Coagulation Factor Deficiency

• Carcinoma
• Endoplasmic Reticulum Stress
• Hereditary Spherocytosis
• Fibrosis
• Hemolysis (disorder)

Interacting Proteins

• CPSF7
• DTX2
• FATE1
• HOXA3
• PRRC1

• SEC24D
• SYNE4

Pathways

• Transport
• Secretory Pathway
• Pathogenesis
• Localization
• Glycosylation
• Protein Transport
• Erythrocyte Development
• Hemopoiesis
• Cytokinesis
• Cell Maturation
• Ribosome Biogenesis
• Translation

• Coagulation
• Chondrocyte Development
• Protein Folding
• Erythrocyte Maturation
• Chromatin Assembly
• Secretion

PTMs

• Glycosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SEC23B

Best Uses Of The SEC23B Marker For Biomedical Research

This article will discuss some of the best uses of the SEC23B marker for biomedical research. Gene infographics and COPII interacting proteins will also be discussed. These methods are applicable to scientists throughout the world. To learn more, read the following articles:

Gene infographics

SEC23B is a gene that provides instructions for a protein called the coat protein complex II (COPII). This gene is involved in the formation of vesicles that carry secretory proteins within the cell. It triggers the formation of COPII vesicles in the endoplasmic reticulum, a part of the cell that processes proteins.

The SEC23B gene contains several mutations. SNPs (single nucleotide polymorphisms) are listed below the physical map. In addition, nonsense and splicing mutations are marked in bold. The SEC23B gene has multiple domains, delineated by numbered amino acids. The COPII complex binding site is inferred from a yeast model.

TEM 1

In TEM-1, an important role is played by the pore-forming enzyme Met-182, which is located far from Leu-76. While Leu-76 is located in the hydrophobic core of the enzyme, it is buried in the a-helical domain, in a helix that contains Ser-70 and Lys-73. Instead, Met-182 lies in the narrow stretch of residues 180, after the omega loop.

In a similar way, enzyme-linked immunosorbent assays (ELISAs) work by using specific antibodies to bind to a target antigen. The detection system then signals that the antigen is bound. The highest sensitivity of an ELISA assay can be achieved when the plates have been coated with high-affinity antibodies. Boster Bio is a leading company in the development of ELISAs and has an impressive track record of developing and manufacturing the newest ELISA kits.

Anti-TEM1 antibodies are readily available in many sources. The antibody is made from rabbits and mice, and reacts with the human protein 'CD248'. It is reported to be 757 amino acids long. Boster Bio Anti-TEM1 is tested in ELISA, Flow Cytometry, IF, and IHC. It is produced by repeated immunization of rabbits with recombinant Human AKT1 and AKT2 proteins.

TEM shows that mitochondria are a primary source of ROS. By staining mitochondria with JC-1, a cell's mitochondrial morphology can be studied. When CD8+ T cells were co-cultured with Huh-7 cells, JC-1 staining decreased and ROS and apoptosis increased. TEM also allows researchers to examine mitochondrial morphology. In the control group, mitochondria had well-defined integral two-layer membranes and regular cristae.

COPII interacting proteins

The COPII interacting proteins (COPI) family includes more than 100 different proteins. In a recent study, Adolf et al. identified 102 proteins in COPI cargo from HeLa cells. Although most of these proteins do not bind GOLPH3, they are all type II Golgi enzymes. It is possible that most of COPI cargo is composed of GOLPH3+3L interactors, which could be broad-spectrum adaptors.

Most secretory cargoes begin in the endoplasmic reticulum, where they are packaged into transport carriers and emerge at defined sites on the ER. Once released, these cargoes fuse with the ER-Golgi intermediate compartment, and the trafficking of these proteins relies on efficient recruitment of soluble factors to the secretory pathway. The COPII coat is composed of two multimeric protein complexes, Sar1 and Sec23. The two proteins interact with each other, resulting in a tight and stable coat.

Using affinity chromatography, these two proteins were identified as major COPI adaptors. The GOLPH3 and GOLPH3L proteins were GST-tagged, allowing them to enrich a wide variety of proteins, including all COPI coat subunits and numerous Golgi-resident glycosylation enzymes. Similarly, immunoblotting showed enrichment of GALNT7 and b-COP.

The COPI coat and GOLPH3 are highlighted in magenta and green, respectively. Their interactions with a host of Golgi-resident vesicular cargo proteins are also highlighted. Data from three biological replicates was analysed using Perseus and Welch's t-tests were used to determine the statistical significance. Interestingly, all four proteins in the table are type II, with the exception of PGAP4 and EBAG9.

TEM 1 interacting proteins

In addition to binding to cells, interacting proteins of the TEM-1 family are important for leukocyte transendothelial migration. These proteins have the ability to tether to phagocytic cells and may be required for efficient trafficking of leukocytes. They may also play a role in the regulation of ERK1/2 and bradykinin receptor activation in endothelial cells and are associated with atherosclerosis.

As a contractual research organization, Boster offers high-affinity antibodies and ELISA kits. The Boster Bio TEM 1 interacting proteins antibody kit is available for research in multiple fields, including neurosciences, developmental biology, and inflammation. Boster Bio TEM 1 interacting proteins antibody kits have been used to screen biomarkers of human diseases and disease. They are derived from proprietary trade secrets. The Boster Bio TEM 1 interacting proteins antibody kit is a versatile and convenient option for determining the protein concentrations of biomarkers and ligands in a variety of tissues, including the blood.

The TEM technique was also used to identify biliary exosomes. These cellular structures are oval or spherical in shape and ranged in size from 40 to 180 nm. Using immunohistochemistry, different proteins associated with the TEM were identified. Positive signals were found between hepatocyte junctions and bile canaliculus. The biotinylated lectins were further detected using SDS-PAGE and Western blotting.

The S protein of nCoV is a trimeric fusion protein that is densely glycosylated. The S protein exists in a metastable prefusion conformation and undergoes substantial structural rearrangement in order to fuse with the host cell membrane. This process is triggered by receptor binding, shedding the S1 subunit and transforming the S2 subunit into a postfusion conformation. As the fusion is completed, the receptor-binding domain of the S protein undergoes hinge-like conformational movements.

TEM 1 sequence

TEM1 antibody, catalog number A00387-1, is a highly sensitive, tunable ELISA antibody that reacts to Human. It has been validated against known positive and negative samples. The company uses proprietary trade secrets to coat plates with high affinity antibodies to achieve maximum sensitivity. Using this process, Boster Bio has become one of the largest antibody catalogs in China. The company offers many different ELISA kits that can be customized to the specifications of individual researchers.