Syndecan-2 Antibodies

Syndecan-2 (SDC2)

Cell surface proteoglycan that bears heparan sulfate. Regulates dendritic arbor morphogenesis (By similarity).

. .

Gene Information

Human
Gene Name:	SDC2
Uniprot:	P34741
Entrez:	6383

Family

Belongs to:
syndecan proteoglycan family

Alternative Names

CD362 antigen; CD362; Fibroglycan; heparan sulfate proteoglycan 1, cell surface-associated; Heparan sulfate proteoglycan core protein; HSPG; HSPG1; HSPG1syndecan proteoglycan 2; SDC2; SYND2; SYND2cell surface-associated heparan sulfate proteoglycan 1; syndecan 2; Syndecan2; Syndecan-2

Molecular Weight

Mass (kDA):
22.16 kDA

Genomic Context

Human
Location:	8q22.1
Sequence:	8; NC_000008.11 (96493601..96611790)

Subcellular Localizations

Membrane; Single-pass type I membrane protein.

Diseases

• Tissue Adhesions
• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Tumor Angiogenesis
• Adenocarcinoma
• Cell Invasion
• Neoplasm Metastasis
• Malignant Tumor Of Colon
• Colonic Neoplasms
• Malignant Paraganglionic Neoplasm
• Cell Transformation, Neoplastic
• Sarcoma

• Fibrosarcoma
• Pathologic Neovascularization
• Prostatic Neoplasms
• Bone Neoplasms
• Malignant Neoplasm Of Prostate
• Neoplasm Invasiveness
• Fibrosis

Interacting Proteins

• CASK
• NF1
• SDC1
• SDC3
• SDC4

Pathways

• Cell Adhesion
• Localization
• Angiogenesis
• Cell Migration
• Cell Proliferation
• Pathogenesis
• Cell Cycle
• Wound Healing
• Cell Growth
• Sulfation
• Regeneration
• Transport
• Brain Development

• Cell Motility
• Cell Differentiation
• Cell-matrix Adhesion
• Leukocyte Differentiation
• Proteolysis
• Cell Cycle Arrest
• Cytoskeleton Organization

PTMs

• Phosphorylation
• Cleavage

• Biotinylation
• Glycosylation

• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SDC2

Best Uses Of The SDC2 Marker

If you're just beginning to learn about Boster Bio, you might be wondering what all the fuss is about. Here you can find troubleshooting and optimization tips as well as guidelines for optimizing Boster bio experiments. It's not a secret that optimizing your experiments can help you get more results in a shorter amount of time. Troubleshooting guides can help you determine the root of any issue.

Boster Bio: High-affinity Primary antibodies

The high-affinity primary antibodies used by Boster Bio are tested for performance in ELISA, Western Blotting, and Immunohistochemistry. They are highly specific and yield reliable, high-quality results. Boster antibodies have been extensively validated for use in the research community for over 25 years. This makes them a highly reliable choice for use in your lab.

The 53-6.7 antibody is an extremely specific monoclonal antigen with broad spectrum binding. It has been proven effective in the analysis of flow cytometry as well as immunoprecipitation as well as staining of frozen tissue sections. In addition it blocks ligand binding which is useful for functional assays. Its purification is optimized to use in ELISA.

Secondary antibodies should be diluted to 1:1000 or less in order to avoid background noise. The dilution of a second antibody can be increased if staining is bright or the readings are too background. After preparation secondary antibodies must be incubated for one hour at 37degC or overnight at 4degC. Boster Bio developed the SDC2 marker to identify primary antibodies with high affinity that targets specific antigens.

Primary antibodies are utilized in direct ways to function as bridges between reporter molecules and the target antigen. Secondary antibodies can be employed to minimize the risk of compromising the reporter's molecule and allow multiplexing. This method also confirms the detection of target antigens. Its limitations include a narrow color palette and a restricted range of molecules that are used as reporter molecules. It is therefore not as efficient as the indirect method. Both methods are viable however it is more practical to choose the direct method when you are required to use more than one types of antibodies.

Troubleshooting guide

This troubleshooting manual can be useful if you are having difficulty using your SDC2 Marker. The suggestions below apply to both mechanical and airsoft markers. These suggestions should not be used in lieu of regular maintenance or replacement. To prevent future problems make sure to carefully inspect and record the airsoft marker once these steps have been completed. Here are some tips and common mistakes that can be used to determine the problem.

The breech could be leaky. First, examine the o rings that seal the HPR. They'll leak if the o-rings aren't seated correctly. If this is the case, you should replace them. Then, you must clean and grease the HPR. Replace any orings that aren't properly seated. If needed, replace them. If that doesn't work, de-gas it before you continue to troubleshoot the problem.

Optimization tips

There are a variety of factors you should consider if you are looking to improve your flow cytometry experiments using the SDC2 marker. It is important to consider the type of sample you're using. If your cells contain both proteins, you can choose a different type of antibody for each staining stage. Flow procedures can be complicated and provide a variety of options. A flow procedure guide will assist you in making the best decision.