Sacsin Antibodies

Sacsin (SACS)

Co-chaperone which functions as a regulator of the Hsp70 chaperone machines and might be involved in the processing of other ataxia-linked proteins. .

Gene Information

Human
Gene Name:	SACS
Uniprot:	Q9NZJ4
Entrez:	26278

Family

Belongs to:
No superfamily

Alternative Names

ARSACS; dnaJ homolog subfamily C member 29; DNAJC29; KIAA0730; SACS; Sacsin; spastic ataxia of Charlevoix-Saguenay (sacsin)

Molecular Weight

Mass (kDA):
521.126 kDA

Genomic Context

Human
Location:	13q12.12
Sequence:	13; NC_000013.11 (23328827..23433728, complement)

Tissue Specificity

Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas.

Subcellular Localizations

Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60.

Diseases

• Ataxia
• Spastic Ataxia, Charlevoix-saguenay Type
• Muscle Spasticity
• Cerebellar Ataxia
• Ataxia, Spinocerebellar
• Neurodegenerative Disorders
• Atrophy
• Hypokinesia
• Peripheral Neuropathy
• Hernia
• Hernia, Inguinal
• Spinocerebellar Degeneration

• Cytogenetic Abnormality
• Early Onset Cerebellar Ataxia
• Infarction
• Myocardial Infarction
• Nerve Degeneration
• Nervousness

Pathways

• Transport
• Excretion
• Pathogenesis
• Cell Death
• Programmed Cell Death
• Intestinal Absorption
• Cholesterol Transport
• Cognition
• Localization
• Protein Folding
• Hibernation
• Muscle Cell Differentiation
• Chaperone-mediated Protein Folding

• Intracellular Cholesterol Transport
• Oxidative Phosphorylation
• Spermatogenesis
• Swimming
• Mitochondrial Transport
• System Development
• Cholesterol Efflux

PTMs

• Phosphorylation
• Oxidation

• Dephosphorylation
• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SACS

Best Uses Of The SACS Marker

This guide will help you if you're looking to use SACS markers in your research. Boster's primary antibodies are cited in the research community and thoroughly validated for Western Blotting, Immunohistochemistry, and ELISA. They are also available in a variety formats. Read on to learn more about Boster's primary antibodies. Here are just a handful:

Boster's primary antibodies are highly cited in the research community

Boster's high-affinity primary antibodies have been used in research for decades. Their primary antibodies are highly cited, and have been extensively validated in ELISA, Western Blotting, and Immunohistochemistry. These reagents can be obtained by other scientists thanks to the detailed product citations. They also serve to validate the product. According to the NIH 54% of all cited publications don't uniquely identify the reagents.

The company offers ANTIHUMAN MAC-2 which is a poly (ADPribose) polymerase, in addition to the p62 antibody. Research is very fond on antibodies that recognize and recognize p62. These antibodies are great for research because they recognize p62 proteins, which are essential components of tumor development and treatment. The p62 monoclonal antibody from Boster is a useful tool in tumor-related research.

Boster's primary antibodies are thoroughly validated on Western Blotting, Immunohistochemistry, and ELISA

Selective PTM-directed antibodies must be validated in multiple ways including immunohistochemistry, Western Blotting, and immunohistochemistry. The antibody may not recognize the target protein if it is not specific for a particular modification. Validation of the PTM directed antibody should be done using separate Western blots with mismatched secondary antibodies to ensure high specificity. Cross-reactive band presence can affect the interpretation and analysis.

The Boster's primary antibodies are rigorously validated for use in ELISA, immunohistochemistry, and Western Blotting. Using human cells, they were incubated overnight at 4 degC. Secondary antibodies were added to the membranes for 1 h at room temperature. Membranes were washed with 1x TBST before imaging. Primary antibodies for GAPDH had been HRP-conjugated to secondary antibodies. The membranes had to be stained and developed using an Optiblot ECL Detectkit and a GBOX XT-16 chemiluminescent scanner.

Independent validation methods include ELISA, immunohistochemistry, and tissue microarrays. These methods employ different antibodies than the Western-blotting antibody. This allows researchers and scientists to verify the reproducibility, as well as the effects, of their research. Further, they provide an added benefit of increasing sensitivity in immunoblotting assays.

WB is a good way to determine the specificity of monoclonal antibodies. WB results cannot be used for absolute proof, however, monoclonal antibodies do not work well in combination with denatured substances. To confirm their specificity, a variety additional techniques such as IHC (Flow cytometry) are used. Negative controls may also be useful to determine if the secondary antibodies are binding not specifically.

It is crucial for reproducibility that primary antibodies are validated. The assay conditions determine the quality of primary antibody. The results can be affected if the assay conditions are not correct. Validating primary antibodies in different conditions is critical. However, this process is not difficult and Boster's primary antibodies are thoroughly validated on Western Blotting, Immunohistochemistry, and ELISA.

To ensure the reproducibility of antibody data, it's important to collaborate with the publisher, vendor, and user. Ideally, antibody users validate their results and design experiments properly. Vendors must provide high quality antibodies and detailed disclosures of methods. Publishers also need to enforce data reporting regulations. The entire community of immunology researchers can improve reproducibility by working together to standardize antibody validation.

ELISA is an enzyme-linked immunosorbent assay that identifies the presence of a specific protein in a complex mixture. Engvall & Perlmann were the first to describe it in 1971. ELISA uses protein samples that have been immobilized in microplatewells. 96-well plates made of polystyrene are commonly used for experiments. These plates passively bind antibodies and protein samples, enabling separation between bound and unbound materials.

Boster's primary antibodies are available in a variety of formats

Boster Bio has a wide range of antibodies, from rabbit polyclonal antibody to validated ELISA reagents. Boster Bio's lab offers highly efficient products that meet all research requirements. Boster products are backed with a strict quality policy and come with a 100 percent satisfaction guarantee. Each antibody will perform exactly as described. Boster antibodies are available in a variety of formats, including lysates, buffers, ELISA reagents, lysates, solutions, and control reagents.

Primary antibodies are essential for immunocytochemistry. They should be used as single-chain antibodies in a laboratory or in a multichain format in immunocytochemistry. Boster's antibodies are standardized and are manufactured with high-quality standards. There are many formats available for boster's reagents. Reconstituted antibodies or reagents can easily be reused multiple times.

Secondary antibodies can be purchased in liquid, powder, or lyophilized concentrate. Secondary antibodies can be stored in the freezer at -20degC for a long period of time before being used. The best method is to aliquot these antibodies before using them. The resulting dilutions will ensure optimal staining. Be sure to take into account the time it takes to reconstitute.

Secondary antibodies can be purchased in two main types: conjugated as well as unconjugated. The intended purpose and the detection method are key factors in deciding which of these two types to use. The most frequently used are alkalinephosphatase or horseradish-peroxidase. Although phosphatase and phosphatase are more expensive but have better long-term signals, alkalinephosphatase is the best option.