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RNF8 Antibodies
AND RNF8 ELISA kits, RNF8 Proteins
Many biological assays use antibodies to detect RNF8, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RNF8 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RNF8 antibodies...
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RNF8 Overview
Facts about E3 ubiquitin-protein ligase RNF8.
E3 ubiquitin-protein ligase RNF8 (RNF8)
E3 ubiquitin-protein ligase that plays an integral role in DNA damage signaling through 2 different functions: by mediating the'Lys-63'- linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing'Lys-48'-linked ubiquitination to eliminate target proteins from DNA damage sites. Following DNA DSBs, it's recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the creation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF).
Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites. Also recruited at DNA interstrand cross-links (ICLs) sites and catalyzes'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link restoration.
Gene Information
| Human | |
|---|---|
| Gene Name: | RNF8 |
| Uniprot: | O76064 |
| Entrez: | 9025 |
Family
| Belongs to: |
|---|
| RNF8 family |
Alternative Names
ActA Binding Protein 2; C3HC4-type zinc finger protein; EC 6.3.2; EC 6.3.2.-; FLJ12013; KIAA0646UBC13/UEV-interacting ring finger protein; ring finger protein (C3HC4 type) 8; ring finger protein 8E3 ubiquitin-protein ligase RNF8; RNF8
Molecular Weight
Mass (kDA):
55.518 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 6p21.2 |
| Sequence: | 6; NC_000006.12 (37353983..37394734) |
Tissue Specificity
Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.
Subcellular Localizations
Nucleus. Cytoplasm. Midbody. Chromosome, telomere. Recruited at uncapped telomeres (By similarity). Following DNA damage, such as double-strand breaks, recruited to the sites of damage (PubMed:18001824, PubMed:18077395, PubMed:22266820, PubMed:23233665). During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be
Diseases
- Malignant Neoplasms
- Genomic Instability
- Neoplasms
- Malignant Neoplasm Of Breast
- Ataxia
- Immunologic Deficiency Syndromes
- Ataxia Telangiectasia
- Telangiectasis
- Genotoxic Stress
- Osteosarcoma
- Cell Transformation, Neoplastic
- Fanconi Anemia
Interacting Proteins
- CEP44
- MAD1L1
- MDC1
- PNMA2
- RBFOX2
- STX11
- TMEM79
- UBE2D2
- VRK1
- WASL
Pathways
- Dna Repair
- Cell Cycle
- Localization
- Histone Ubiquitination
- Dna Damage Checkpoint
- Double-strand Break Repair
- Mitosis
- Spermatogenesis
- Cell Division
- Protein Phosphorylation
- Cell Cycle Arrest
- Conjugation
- Chromatin Remodeling
PTMs
- Phosphorylation
- Ubiquitination
- Acetylation
- Methylation
Research Areas
- Stem Cell Markers
- Zinc Finger
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RNF8
bosterbio.com/bosterbio-gene-info-cards/RNF8
References
PMID: 9852682 by Seki N., et al. Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif.
PMID: 16215985 by Plans V., et al. The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation.