Rnf128 Antibodies

E3 ubiquitin-protein ligase RNF128 (Rnf128)

E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription.

Inhibits IL2 and IL4 transcription, thereby playing a significant role in the induction of the anergic phenotype, a long-term steady state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with'Lys- 48' linkages and COR1A with'Lys-63' linkages leading to their degradation, down-regulation of those cytosleletal components leads to impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse.

Gene Information

Mouse
Gene Name:	Rnf128
Uniprot:	Q9D304
Entrez:	66889

Family

Belongs to:
No superfamily

Alternative Names

EC 6.3.2; EC 6.3.2.-; FLJ23516; Gene related to anergy in lymphocytes protein; GRAIL; ring finger protein 128GRAILE3 ubiquitin-protein ligase RNF128; RNF128

Molecular Weight

Mass (kDA):
46.276 kDA

Genomic Context

Mouse
Location:	X|X F1
Sequence:	X;

Tissue Specificity

Expressed in brain, kidney, heart, liver, ovary, testis and thymus. Expression increased as early as 4 hours by 5- to 7-fold in anergized cultures as compared to resting or activated cells.

Diseases

• Autoimmune Diseases
• Autoimmunity
• Autoimmune Reaction
• Radiation Chimera Disorder

Pathways

• Cell Activation
• Tolerance Induction
• Cytokine Production
• T Cell Activation
• T Cell Anergy
• T Cell Tolerance Induction

PTMs

• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Rnf128

Boster Bio - Best Uses Of The RNF128 Marker

If you're looking for antibodies that target the RNF128 Marker, Boster Bio may be the right place for you. Boster Bio has validated antibodies using both positive and negative samples to ensure high specificity and affinity. They also reward the first reviewers of their products with product credits, which they distribute to scientists worldwide. Interested in learning more? Check out this informative article.

Boster validates antibodies on WB

Boster Bio is a manufacturer and distributor of quality reagents, biomarker kits, and lysates. Their antibodies can detect a wide variety of biomarkers in a broad range industries, including neurosciences and cancer, inflammation, developmental biology, and inflammation. Boster Bio also offers a variety of control antibodies and solutions as well as extraction kits. Boster Bio also sells a wide range of ELISA kits and buffers as well as lysates.

Boster's antibodies were developed over 25-years and are well validated on ELISA/IHC, and WB. Boster's rabbit polyclonal antibodies also include a free secondary antibody with every primary. They are available for human and mouse samples. The antibodies are stable and retain biological activity for multiple uses including Western blotting and ELISA.

Boster Bio conducts numerous validations on its products using known positive as negative samples in order to ensure the quality of their product. This results is a high level of specificity and affinity for each agent. In addition to rigorous testing, Boster rewards the first reviewer of its products with a free product. Boster rewards all scientists for their contribution in scientific research. They are dedicated to delivering quality reagents for the life sciences industry.

IHC

The RNF128 IHC marker is an important component. It is part of several other genes in the intestine. These genes include Caudal Type Homeobox 2 - Cdx2, Omega-3 Fatty A Receptor 1 - O3far1, Forkhead F2 - F2 (Foxf2), Early B cell factor 1 – Tspan8.

The BE cell line expresses reduced levels of RNF128 isoform 2 in contrast to isoform 1 in the control group. The decreased isoform 2 results in increased levels of mutant p53 while isoform 1 promotes its stabilization. Both proteins are involved in the degradation of the p53 gene. This is an essential component of the immune response. The progression of BE or HGD can also be detected using the RNF128 marker.

ICC

The ICC uses for the RNF128 marker protein marker were mainly based on a recent study that used human melanoma cells. These cell lines were cultured in DMEM, RPMI-1640 medium with 10% fetal bovine serum, and a CO2 incubator of 5%. Moreover, pLVX-shRNA-eGFP-PGK puro lentiviral vectors were purchased from Genomeditech as negative controls.

This study reveals that a new drug can be used to treat unresectable ICC. Anlotinib prolongs overall survival and prevents progression. Its clinical application is urgently required. There are many unknowns about the drug's mechanism of action and how it works. These results indicate that the RNF128 marker may offer some benefits. These results should be used by doctors to determine if a new drug is possible to treat patients with ICC.

The deletion of the RING finger domain in T cells reduces the unresponsiveness of T cells. As a result, the immune cells lose the ability to maintain inactive states and promote proliferation and survival of self-reactive lymphocytes. RNF128 is also known to increase the activation and proliferation primary CD4+T cells. RNF128 may not be useful for predicting tumorigenesis.

The study involved the use of two human ICC cell line HCC9810, and RBE. The cell lines were transfected with HA-tagged ubiquitin antibody transiently in RPMI 1640. After transfection, the cells were washed in PBS and incubated for 10 min with MG132. The cells were then analyzed for tumor growth and volume 10 minutes after transfection.

Immunofluorescence

The RNF128 biomarker is widely used to detect proteins. This marker can be used for a variety of purposes, including cancer immunotherapy. It has been labeled recently with a fluorescent reporter colour. Fluorescent signals can be analysed using flow cytometric methods. This marker is an excellent choice for cancer immunotherapy. This marker is a powerful tool that can help you detect cancer cells resistant to other treatments.

The RNF128 marker is an antibody that recognizes specific proteins. It can recognize multiple epitopes, but they don't have to be in the same place to be recognized by antibodies. The RNF128 markers are used for many research purposes. Here are some of its best uses. They include: [*]