ROCK2 Antibodies

Rho-associated protein kinase 2 (ROCK2)

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility through phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM.

Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation.

Gene Information

Human
Gene Name:	ROCK2
Uniprot:	O75116
Entrez:	9475

Family

Belongs to:
protein kinase superfamily

Alternative Names

coiled-coil-containing protein kinase 2; EC 2.7.11; EC 2.7.11.1; KIAA0619; p164 ROCK-2; Rho kinase 2; rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; ROCK2; ROCK-II; ROKalpha

Molecular Weight

Mass (kDA):
160.9 kDA

Genomic Context

Human
Location:	2p25.1
Sequence:	2; NC_000002.12 (11179759..11345437, complement)

Subcellular Localizations

Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Diseases

• Tissue Adhesions
• Malignant Neoplasms
• Hypertensive Disease
• Neoplasms
• Cardiovascular Diseases
• Cell Invasion
• Inflammation
• Carcinoma
• Neoplasm Metastasis
• Pathologic Vasoconstriction
• Neoplasm Invasiveness
• Pulmonary Hypertension
• Atherosclerosis

• Fibrosis
• Hypoxia
• Hypertrophy
• Cell Transformation, Neoplastic
• Malignant Squamous Cell Neoplasm
• Malignant Neoplasm Of Breast
• Mammary Neoplasms

Interacting Proteins

• JAK2
• STAT3

Pathways

• Cell Migration
• Pathogenesis
• Cell Motility
• Cell Adhesion
• Vasoconstriction
• Cell Proliferation
• Cell Cycle
• Cytokinesis
• Sensitization
• Angiogenesis
• Muscle Contraction
• Smooth Muscle Contraction
• Cell Death

• Localization
• Secretion
• Cell Differentiation
• Translation
• Cell Growth
• Regeneration
• Transport

PTMs

• Phosphorylation
• Cleavage
• Dephosphorylation

• Amidation
• Methylation
• Geranylgeranylation

• Ubiquitination

Research Areas

• Breast Cancer
• Cancer
• Protein Kinase
• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ROCK2

Boster Bio - Best Uses Of The ROCK2 Marker

Boster Bio's AntiROCK2 antibody may interest you as a biomarker researcher. This article will discuss the validation and application. Continue reading to learn more. To learn more visit Boster's site. Boster will reward the first person to review the product with product credits. This is a great incentive for all scientists.

Boster Bio Anti-ROCK2 antibody

Boster Bio offers a commercial anti-ROCK2 antibody which is highly reactive to Human, Mouse, and Rat. The antibody is derived from rabbit immunization with a synthetic protein. It is suitable for WB applications and is available in a PBS solution containing 0.09% sodium azide. The product can be stored at -20°C for a period of six month. The Boster Bio Anti-ROCK2 antibody has 4 mg of Trehalose per vial. The peptides used in blocking the anti-ROCK2 antibody are available for purchase on their own.

ROCK2 is known for its activation during Apoptosis. Rapamycin stimulation stimulated rapid transfer of FKBP-p63RhoGEF DH to the LDR. The FRET/CFP ratio in HeLa cells increased gradually over three to four hours, then followed by a rapid rise and rapid drop. Cells also showed blebbing of the cell membrane.

Different biological tests can utilize the antibodies that detect ROCK2. The antibodies can be monoclonal or multiclonal , and work well with various animal samples. Boster Bio produces anti-ROCK2 antibodies using mouse and rabbit samples. This antibody binds to Rho-associated proteins Kinase, a signaling proteins that regulates smooth muscle contractions and cell polarity.

Boster Bio AntiROCK2 antibody is the most sensitive and selective of all anti-ROCK2 commercially available antibodies. Its high sensitivities make it a fantastic instrument for imaging studies that involve ROCK activity. This antibody can also be used in biosensors for the detection of the kinases of human and mouse ERK, and BTK. This antibody can be useful for diagnosis and research. It also can be used to motivate researchers to develop biosensors to other protein kinases.

The phosphorylated antibody is an interacting partner for a range of targets. These include phosphorylated vimentin total vimentin and p-vimentin. ROCK2 also regulates adhesion molecules ' expression as well as vimentin colocalization. It is possible to use ROCK2 phosphorylated antibodies for different research objectives.

ROCK2 is an essential protein needed by healthy cells. In humans, it has numerous functions in the body including regulating immune response to apoptosis, apoptosis, as well as cancer treatment. The anti-ROCK2 antibody, despite its significance in human research, is not widely used. The most effective applications for ROCK2 will be discovered in cancer research, diabetes and inflammatory diseases.

Validation

This gene is an important marker in the apoptosis pathway of the alveolar epithelial cell (AEC) which is a key constituent of the lung. It has been associated with pulmonary fibrillation in mouse models. ROCK1 is also involved in the activation of apoptosis machinery and its inhibition may restore immune system homeostasis.

ROCK2 is involved in the formation of endothelial tissues. It is expressed during embryonic growth and regulates TGF-beta signaling. It also inhibits blood coagulation. ROCK2 loss can trigger the formation of thrombuses, as well as intrauterine growth retardation as well as the death of the fetus as well as placental dysfunction. Thus, this gene is essential to the development of embryos. Multiple mouse strains were utilized to test the ROCK2 gene.

Y27632 and SR3677 have a higher affinity for ROCK2 than ROCK1. However, both have low IC50 for ROCK1 and have less selectivity for ROCK2. Both inhibitors are able to affect the brain and have negative effects on non-neuronal tissue. It is crucial to create an ROCK2 inhibitor with greater sensitivity for clinical use. It is important to understand how this gene works in neuronal cells to develop antifibrotic agents.

This study suggests that ROCK2 is an important marker for pulmonary the fibrosis. The ROCK2 gene is involved in a myriad of ways that fibrosis occurs and its slight reduction could help to regulate the activity of IPF while recovering non-fibrotic functions. This gene may be a successful therapeutic approach to treat pulmonary fibrosis. So, the next step is to find out what role it plays in IPF.

Lung collagen is defined by its content of hydroxyproline. This study revealed that ROCK1+/-2+/ mouse were protected from lung fibrosis that is caused by bleomycin. The WT mice had a lower total lung hydroxyproline. It increased by 91% in ROCK1+/-2+/ mice when compared to the baseline. Further research is needed to determine which ROCK is responsible in lung fibrosis.

ROCK isoforms are crucial in the cell response. The activation of fibroblasts by Bleomycin triggers differentiation into myofibroblasts. Myofibroblasts produce a-SMA, which is a marker for myofibroblast differentiation. A-SMA is found in smooth muscle cells that connect blood vessels and airways. These genes are closely connected.

Application

The ROCK2 Marker is a biomarker able to be used in a variety of clinical settings. ROCK2 is involved in the regulation of centrosomal duplication in many cellular settings. It is also widely known to play part in apoptosis and cell death as well as the production of reactive oxygen substances. It is also an effective regulator of cell adhesion, migration and. The use of the ROCK2 Marker is a valuable method to identify cardiovascular events and may provide an opportunity to treat.

ROCK2 isozyme has been found to inhibit regulatory T cells' function. It has been shown to positively regulate the expression of the forkhead-box and STAT5 phosphorylation. In mice, ROCK2 down-regulates the development of arthritis induced by collagen. The application of the ROCK2 Marker could help restore the equilibrium of the immune system. ROCK2 targeting is an important tool for the treatment of autoimmunity as well as other immune disorders.

The ROCK2 Marker has been found to be high-yielding in cutaneous lesions of several autoimmune diseases. It has also been linked to vascular remodeling. It activates myosinATPase in order to stimulate smooth muscle movement and motility. The clinical manifestations of autoimmune connective disorder syndromes are influenced by vascular inflammation and inflammation. Numerous studies have looked into the role ROCK2 plays in the process of vascular remodelling.

The activation of ROCKs is a result of interaction between the RBD domain with GTP-loaded RhoA. The activation of ROCKs gets fine-tuned through autophosphorylation. The activation of ROCKs is essential for the regulation of cell function. It is important that ROCK1 and ROCK2 are downstream targets for RhoA, TFH and SHP2.

Radioresistance is still a major hurdle in the successful treatment of various kinds of tumors. Numerous intrinsic factors affect the response of the tumor to radiation. In vitro and clinical sample-based studies of cervical cancer have investigated RhoC. The research has shown that RhoC is involved in radioresistance. ROCK2 inhibition hinders DNA repair pathway proteins, such as MRN complex, pH2Ax, and MRN which are crucial in preventing double-strand break.

Inhibition of ROCK decreases apoptosis and cardiomyocyte hypertrophy. The inhibition of ROCK inhibits Bcl-2 family protein Bax, which prevents the development of cardiac fibrosis and reduces the vascular resistance. However the pharmacological inhibition ROCK is crucial for progressing medical research and clinical care. Pharmacological inhibition of ROCK1 or ROCK2 reduces the gene's activity, and improves health.