RFWD3 Antibodies

AND RFWD3 ELISA kits, RFWD3 Proteins

Many biological assays use antibodies to detect RFWD3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RFWD3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RFWD3 antibodies...

We validate the specificity of these antibodies to RFWD3 by testing them on tissues known to express RFWD3 positively and negatively. Browse below to find the RFWD3 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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RFWD3 Infographic by Boster Bio

All RFWD3 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-RFWD3Antibody

4 Q&As

Human

$360
Cat # A09738
100 µl

Anti-RFWD3 Antibody

Human, Mouse, Rat

$405
Cat # A09738-1
100ul

RFWD3 (NM_018124) Human Over-expression Lysate

Human

$628
Cat # LS055159
100 µg

RFWD3 Overview

Facts about E3 ubiquitin-protein ligase RFWD3.

RFWD3 3D structure. Source: alphafold

E3 ubiquitin-protein ligase RFWD3 (RFWD3)

E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA- mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658).

Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to eliminate them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098).

Gene Information

Human
Gene Name:	RFWD3
Uniprot:	Q6PCD5
Entrez:	55159

Family

Belongs to:

No superfamily

Alternative Names

EC 6.3.2.-; FLJ10520; ring finger and WD repeat domain 3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201; RNF201E3 ubiquitin-protein ligase RFWD3

Molecular Weight

Mass (kDA):
85.094 kDA

Genomic Context


Human

Location:	16q23.1
Sequence:	16; NC_000016.10 (74621396..74666892, complement)

Subcellular Localizations

Nucleus. Nucleus, PML body. Cytoplasm. In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies (PubMed:21558276). In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair (PubMed:21504906, PubMed:28575657). This recruitment depends upon RPA2 (PubMed:21504906).